EIF4ENIF1 (NM_019843) Human Tagged ORF Clone

CAT#: RC205985

EIF4ENIF1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1

Product Images

Circular map for RC205985

• 

  Western blot validation of overexpression lysate (Cat# LY432079) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229052 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF4ENIF1
• Synonyms: 4E-T; Clast4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205985 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGGAGAAGTATGGGTGAAACAGAAAGTGGAGATGCTTTCCTTGACCTGAAGAAGCCTCCTGCCT
  CCAAATGCCCCCATCGCTATACAAAAGAAGAACTCTTGGATATAAAAGAACTCCCCCATTCCAAACAGAG
  GCCTTCATGCCTTTCTGAAAAATATGACAGTGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTC
  TACCCAGCTTCAGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTTGGATACAGACCGGCCTTCCC
  TGGTGCGCAGGATAGTAGATCCACGAGAGCGTGTGAAAGAAGATGACTTAGATGTTGTTCTCAGCCCTCA
  GAGACGGAGCTTTGGAGGGGGCTGCCACGTGACAGCCGCTGTTAGCTCCCGGCGCTCAGGAAGTCCATTA
  GAGAAAGATAGTGATGGGCTTCGTCTGCTTGGTGGACGTAGGATTGGCAGTGGGAGGATAATCTCTGCCC
  GGACCTTTGAGAAGGATCACCGTCTTAGCGATAAGGACCTGCGGGACTTGAGAGACAGAGACCGAGAGAG
  GGACTTCAAGGACAAGCGTTTCAGGAGAGAGTTTGGAGATAGTAAGCGTGTCTTTGGTGAGCGTAGAAGA
  AATGATTCTTACACAGAAGAAGAACCAGAGTGGTTCTCTGCTGGACCCACAAGTCAGTCTGAAACCATCG
  AACTGACTGGCTTTGATGATAAGATACTAGAAGAAGATCACAAAGGGAGAAAAAGAACAAGGCGACGGAC
  AGCCTCTGTGAAGGAAGGTATAGTAGAGTGCAATGGAGGAGTGGCCGAAGAGGATGAAGTGGAGGTCATC
  CTTGCACAGGAGCCTGCGGCTGATCAGGAAGTGCCAAGGGATGCTGTCTTGCCTGAGCAGTCCCCAGGAG
  ACTTTGACTTTAATGAGTTCTTTAACCTTGATAAGGTGCCATGCTTGGCTTCGATGATAGAAGATGTTTT
  GGGAGAAGGGTCAGTCTCTGCCAGTCGGTTCAGTAGGTGGTTCTCTAACCCGAGCAGATCAGGAAGCCGA
  TCCAGCAGTCTTGGGTCAACACCACATGAAGAGCTAGAGAGACTTGCAGGTCTGGAGCAAGCCATCCTCT
  CTCCTGGACAGAACTCGGGGAATTACTTTGCTCCTATACCATTGGAAGACCATGCTGAAAATAAAGTGGA
  TATTTTAGAAATGCTACAGAAAGCCAAAGTGGATTTGAAACCTCTTCTTTCCAGCCTTTCTGCAAATAAA
  GAAAAACTTAAAGAAAGCTCACATTCAGGGGTTGTGCTTTCAGTGGAGGAGGTAGAAGCAGGTCTGAAGG
  GCTTGAAGGTTGACCAGCAAGTGAAGAATTCAACTCCCTTCATGGCAGAACACCTAGAAGAGACCTTGAG
  TGCCGTAACCAACAATCGACAACTGAAGAAAGACGGAGACATGACTGCGTTCAACAAGCTAGTGAGCACA
  ATGAAGGCAAGTGGGACTTTGCCTTCTCAGCCCAAAGTCAGCCGAAACCTTGAAAGCCATTTGATGTCCC
  CTGCTGAGATTCCAGGCCAGCCTGTCCCTAAGAACATCCTGCAGGAACTTCTGGGTCAACCAGTTCAGAG
  ACCTGCTTCTTCCAATCTTCTGAGTGGCCTTATGGGGAGCTTGGAGCCTACAACATCTTTACTGGGCCAA
  AGAGCACCCTCTCCTCCCTTGTCACAGGTGTTTCAAACTCGAGCAGCCTCAGCTGACTACCTTCGCCCAA
  GAATACCATCACCAATTGGTTTCACACCAGGACCACAGCAGCTACTCGGAGATCCATTCCAAGGCATGCG
  CAAACCCATGAGCCCCATCACAGCCCAGATGAGCCAGCTGGAGTTGCAACAGGCAGCTTTAGAAGGGCTG
  GCCTTGCCACATGACCTTGCTGTACAGGCAGCAAACTTCTACCAGCCTGGTTTTGGCAAACCACAGGTGG
  ACAGAACCAGAGATGGATTCAGAAACAGGCAACAGCGAGTGACCAAGTCACCAGCACCCGTGCATCGAGG
  GAATTCCTCTTCCCCTGCCCCTGCTGCCTCCATCACAAGCATGCTTTCTCCTTCCTTTACCCCTACCTCA
  GTGATTCGTAAGATGTACGAGAGCAAAGAGAAAAGCAAGGAGGAGCCAGCATCTGGAAAAGCAGCTCTTG
  GTGACAGTAAAGAGGATACTCAGAAGGCCAGTGAAGAAAACCTCCTGTCATCCAGCTCTGTACCCAGTGC
  CGATCGAGACTCTTCTCCCACTACAAATTCCAAACTGTCAGCATTACAGAGGTCTTCGTGTTCCACCCCA
  CTGTCCCAGGCCAACCGTTACACCAAAGAACAAGATTATCGACCTAAAGCAACTGGGAGAAAAACACCCA
  CCTTGGCATCCCCAGTTCCTACAACACCTTTTCTCCGCCCTGTCCACCAAGTTCCCCTTGTCCCCCATGT
  CCCTATGGTTAGGCCTGCTCACCGGCTTCACCCAGGGTTGGTACAGAGGATGCTGGCCCAGGGAGTACAT
  CCACAGCATCTTCCAAGTTTGCTCCAAACTGGTGTGCTTCCTCCTGGGATGGACTTGAGTCATTTACAGG
  GAATATCTGGCCCCATCCTGGGTCAGCCCTTTTACCCTTTACCTGCTGCTAGTCACCCTCTCTTAAACCC
  TCGTCCTGGAACACCTCTGCATCTGGCAATGGTGCAACAGCAGCTACAGCGCTCAGTTCTGCATCCTCCA
  GGCTCTGGTTCCCATGCAGCAGCTGTCAGCGTTCAGACAACCCCTCAGAACGTGCCCAGCCGGTCAGGCC
  TGCCCCACATGCACTCCCAGCTGGAGCATCGCCCCAGCCAGAGGAGCAGCTCCCCTGTGGGCCTTGCCAA
  ATGGTTTGGCTCAGATGTGCTACAGCAACCCCTGCCCTCCATGCCCGCCAAAGTTATCAGTGTAGATGAA
  TTGGAATACCGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205985 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASL
  YPASGRSSPVESLKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPL
  EKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRR
  NDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVI
  LAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSR
  SSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANK
  EKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVST
  MKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKNILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQ
  RAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGPQQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGL
  ALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTS
  VIRKMYESKEKSKEEPASGKAALGDSKEDTQKASEENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTP
  LSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFLRPVHQVPLVPHVPMVRPAHRLHPGLVQRMLAQGVH
  PQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFYPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPP
  GSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDE
  LEYRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019843
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019843.4
• RefSeq Size: 3704 bp
• RefSeq ORF: 2958 bp
• Locus ID: 56478
• UniProt ID: Q9NRA8
• Cytogenetics: 22q12.2
• MW: 108.2 kDa
• Gene Summary: The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]