EIF4ENIF1 Rabbit Polyclonal Antibody

CAT#: TA375834

EIF4ENIF1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using EIF4ENIF1 antibody (TA375834) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

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  Immunofluorescence analysis of NIH/3T3 cells using EIF4ENIF1 Rabbit pAb (TA375834) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using EIF4ENIF1 Rabbit pAb (TA375834) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using EIF4ENIF1 Rabbit pAb (TA375834) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EIF4ENIF1 (NP_001157973.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 88kDa/108kDa
• Gene Name: eukaryotic translation initiation factor 4E nuclear import factor 1
• Database Link:
  Entrez Gene 56478 Human
  UniProt ID Q9NRA8
• Background: The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 4E-T; 2610509L04Rik; Clast4; eIF4E-transporter; FLJ21601; FLJ26551; OTTHUMP00000063276