ABHD4 (NM_022060) Human Tagged ORF Clone

CAT#: RC205745

ABHD4 (Myc-DDK-tagged)-Human abhydrolase domain containing 4 (ABHD4)

Product Images

Circular map for RC205745

• 

  Western blot validation of overexpression lysate (Cat# LY411808) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205745 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD4
• Synonyms: ABH4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205745 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGATGATCTGGAGCAGCAGTCTCAAGGCTGGCTGAGTAGCTGGCTGCCCACGTGGCGCCCCACTT
  CCATGTCTCAGCTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAATAAGTTCCTGGCCAGATA
  TGTATCCCTCCCAAACCAGAATAAGATCTGGACGGTGACTGTGAGCCCCGAGCAAAACGACCGCACCCCC
  TTGGTGATGGTGCATGGTTTTGGGGGCGGCGTGGGTCTCTGGATCCTCAACATGGACTCACTGAGTGCCC
  GCCGCACACTGCACACCTTCGATCTGCTTGGCTTCGGGCGAAGCTCAAGGCCAGCATTCCCAAGGGACCC
  GGAGGGGGCTGAGGATGAGTTTGTGACATCGATAGAGACATGGCGGGAGACCATGGGGATCCCCAGCATG
  ATCCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCAATCAAGTACCCTGATAGAGTTA
  AACACCTCATCCTGGTGGACCCATGGGGCTTTCCCCTCCGACCAACTAACCCCAGTGAGATCCGTGCACC
  CCCAGCCTGGGTCAAAGCCGTGGCATCTGTCCTAGGACGTTCCAATCCATTGGCTGTTCTTCGAGTAGCT
  GGGCCCTGGGGGCCTGGTCTGGTGCAGCGATTCCGGCCGGACTTCAAACGCAAGTTTGCAGACTTCTTTG
  AAGATGATACCATATCAGAGTATATTTACCACTGCAACGCACAGAATCCCAGTGGTGAGACAGCATTCAA
  AGCCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCTATGCTGGAGCGAATTCACTTGATTCGAAAAGAT
  GTGCCTATCACTATGATCTACGGGTCCGACACCTGGATAGATACCAGTACGGGAAAAAAGGTGAAGATGC
  AGCGGCCGGATTCCTATGTCCGAGACATGGAGATTAAGGGTGCCTCCCACCATGTCTATGCTGACCAGCC
  ACACATCTTCAATGCTGTGGTGGAGGAGATCTGCGACTCAGTTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205745 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTP
  LVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSM
  ILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVA
  GPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKD
  VPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022060
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022060.2, NP_071343.2
• RefSeq Size: 2543 bp
• RefSeq ORF: 1029 bp
• Locus ID: 63874
• UniProt ID: Q8TB40
• Cytogenetics: 14q11.2
• Domains: abhydrolase
• Protein Families: Protease
• MW: 38.8 kDa
• Gene Summary: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.[UniProtKB/Swiss-Prot Function]