DIS3L2 (NM_152383) Human Tagged ORF Clone

CAT#: RC205512

DIS3L2 (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae)-like 2 (DIS3L2)

Product Images

Circular map for RC205512

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  Western blot validation of overexpression lysate (Cat# LY407628) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205512 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DIS3L2 protein (Cat# TP305512). The protein was produced from HEK293T cells transfected with DIS3L2 cDNA clone (Cat# RC205512) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DIS3L2
• Synonyms: FAM6A; hDIS3L2; PRLMNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205512 representing NM_152383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCATCCTGACTACAGAATGAACCTCCGGCCCCTGGGGACCCCCAGAGGTGTGTCTGCTGTGGCTG
  GTCCACATGACATTGGTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAGGTCCACACGAGGGAAGAAAAA
  GAGCATATTTGAAACTTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTCATCCAG
  GGTGTATTGAGAATTAATCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCCCCGGATGGTGATCGAGACA
  TTTTTATTGATGGGGTTGTTGCTCGTAATAGAGCCTTAAATGGGGATCTGGTGGTCGTGAAACTGCTTCC
  CGAGGAGCATTGGAAGGTAGTTAAACCAGAGAGCAATGACAAAGAAACAGAAGCTGCGTATGAATCAGAT
  ATCCCCGAGGAGCTCTGTGGACACCATCTCCCGCAACAGTCCCTGAAAAGCTATAATGACAGTCCTGATG
  TCATTGTAGAGGCTCAGTTTGATGGCAGCGACTCAGAAGATGGACATGGCATCACACAAAATGTGCTGGT
  TGATGGTGTTAAGAAACTCTCAGTTTGTGTTTCTGAGAAAGGAAGAGAGGATGGTGATGCACCGGTTACA
  AAAGATGAGACCACCTGCATTTCACAAGACACAAGAGCTTTATCGGAGAAATCCCTGCAAAGATCAGCAA
  AGGTGGTTTACATCTTGGAGAAAAAACATTCTCGAGCAGCAACCGGCTTCCTCAAACTCTTGGCTGATAA
  GAACAGCGAACTGTTTAGGAAATACGCCCTGTTTTCTCCCTCAGACCACCGAGTGCCTAGAATTTATGTG
  CCTCTCAAGGACTGTCCCCAGGACTTTGTGGCACGGCCTAAAGATTATGCCAACACACTGTTCATCTGCC
  GCATTGTGGACTGGAAGGAGGACTGCAATTTTGCCCTGGGGCAGCTGGCTAAGAGTCTTGGGCAGGCTGG
  TGAAATTGAGCCTGAAACAGAAGGAATACTAACAGAGTATGGCGTGGATTTCTCTGATTTCTCTTCAGAA
  GTTCTAGAATGTCTTCCTCAAGGCCTGCCATGGACAATTCCACCAGAGGAGTTCAGCAAGAGAAGGGATT
  TAAGAAAAGACTGTATCTTCACCATTGACCCATCAACCGCCCGAGACCTCGATGATGCCCTCTCCTGCAA
  GCCACTCGCTGACGGCAACTTCAAAGTGGGAGTTCACATTGCTGACGTGAGTTACTTTGTTCCGGAGGGA
  TCTGATCTGGATAAAGTGGCTGCCGAGAGGGCTACAAGCGTCTACTTGGTTCAAAAGGTGGTCCCCATGC
  TTCCCAGGCTGCTGTGTGAGGAGCTGTGCAGCCTCAACCCCATGTCCGACAAGCTGACCTTCTCTGTGAT
  CTGGACACTGACTCCAGAGGGCAAGATCCTTGATGAATGGTTTGGCCGGACCATCATCCGCTCCTGCACC
  AAACTTAGCTACGAGCATGCACAGAGCATGATTGAAAGCCCAACTGAGAAAATCCCTGCGAAAGAGCTGC
  CCCCCATTTCCCCAGAGCATAGCAGCGAGGAGGTACACCAGGCCGTCTTGAATCTCCACGGAATTGCCAA
  GCAGTTACGCCAGCAGCGCTTTGTGGACGGCGCACTTCGTTTGGATCAGCTAAAGCTTGCTTTCACTCTG
  GACCACGAGACCGGATTGCCTCAAGGATGTCATATCTATGAGTACCGCGAGAGCAACAAGCTCGTGGAGG
  AGTTCATGCTCTTGGCCAACATGGCAGTGGCCCACAAGATCCACCGCGCCTTCCCCGAGCAGGCCCTGCT
  GCGCCGGCACCCCCCGCCCCAAACAAGGATGCTCAGTGACCTGGTGGAATTCTGCGACCAGATGGGGCTG
  CCCGTGGACTTCAGCTCCGCAGGAGCCCTCAATAAAAGCCTGACCCAAACATTTGGAGATGACAAGTACT
  CACTGGCCCGCAAGGAGGTGCTCACCAACATGTGCTCCCGGCCCATGCAGATGGCACTGTACTTCTGCTC
  GGGGCTGCTGCAGGACCCAGCGCAGTTCCGGCACTACGCGCTCAATGTGCCCCTGTACACACACTTCACC
  TCGCCCATCCGCCGCTTTGCCGACGTCCTGGTGCACCGCCTCCTGGCTGCCGCGTTAGGCTATAGGGAGC
  GACTAGACATGGCGCCCGATACCCTGCAGAAACAGGCGGACCACTGTAACGACCGCCGCATGGCGTCCAA
  GCGCGTGCAGGAGCTCAGTACCAGTCTCTTCTTTGCTGTTCTGGTCAAGGAGAGTGGCCCCCTGGAGTCA
  GAAGCCATGGTGATGGGCATCCTGAAGCAAGCCTTCGACGTGCTGGTGCTGCGCTACGGCGTGCAGAAGC
  GCATCTACTGCAACGCACTGGCCCTGCGGTCCCACCACTTCCAGAAGGTGGGCAAGAAGCCGGAACTCAC
  GCTGGTCTGGGAGCCTGAGGACATGGAGCAGGAGCCAGCACAGCAGGTCATCACCATCTTCAGCCTGGTG
  GAGGTGGTCCTGCAGGCAGAGTCCACAGCCCTCAAGTACAGCGCCATCCTGAAGCGGCCAGGCACCCAGG
  GCCACCTGGGCCCTGAGAAGGAGGAGGAGGAGTCTGACGGTGAGCCCGAGGACTCAAGCACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205512 representing NM_152383
  Red=Cloning site Green=Tags(s)
  
  MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQ
  GVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESD
  IPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVT
  KDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYV
  PLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSE
  VLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEG
  SDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCT
  KLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFVDGALRLDQLKLAFTL
  DHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAFPEQALLRRHPPPQTRMLSDLVEFCDQMGL
  PVDFSSAGALNKSLTQTFGDDKYSLARKEVLTNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFT
  SPIRRFADVLVHRLLAAALGYRERLDMAPDTLQKQADHCNDRRMASKRVQELSTSLFFAVLVKESGPLES
  EAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLV
  EVVLQAESTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152383
• ORF Size: 2655 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152383.5
• RefSeq Size: 3507 bp
• RefSeq ORF: 2658 bp
• Locus ID: 129563
• UniProt ID: Q8IYB7
• Cytogenetics: 2q37.1
• MW: 99.1 kDa
• Gene Summary: The protein encoded by this gene is similar in sequence to 3'/5' exonucleolytic subunits of the RNA exosome. The exosome is a large multimeric ribonucleotide complex responsible for degrading various RNA substrates. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Mar 2012]