SPOCK1 (NM_004598) Human Tagged ORF Clone

CAT#: RC205296

SPOCK1 (Myc-DDK-tagged)-Human sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 (SPOCK1)

Product Images

Circular map for RC205296

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPOCK1
• Synonyms: SPOCK; TESTICAN; TIC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205296 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCCGGCGATCGCGGTGTTGGCGGCGGCCGCCGCGGCGTGGTGCTTCCTCCAAGTCGAGAGCCGGCACC
  TGGACGCGCTCGCCGGAGGCGCGGGCCCCAACCACGGCAATTTCCTAGACAATGACCAGTGGCTGAGCAC
  CGTCTCCCAGTACGACCGGGACAAGTACTGGAACCGCTTTCGAGACGATGATTATTTCAGAAACTGGAAT
  CCCAACAAGCCCTTTGACAAAGCCCTGGACCCATCCAAGGACCCCTGCCTGAAGGTAAAATGCAGCCCTC
  ACAAAGTGTGTGTGACCCAGGACTACCAGACCGCCCTGTGTGTCAGCCGCAAGCACCTGCTCCCCAGGCA
  AAAGAAGGGGAACGTGGCCCAGAAACACTGGGTTGGACCTTCGAATTTGGTCAAGTGCAAGCCCTGTCCC
  GTGGCACAGTCAGCCATGGTCTGCGGCTCAGATGGCCACTCCTACACATCCAAGTGCAAATTGGAGTTCC
  ATGCTTGTTCTACTGGCAAAAGCCTCGCCACCCTCTGTGATGGGCCCTGTCCCTGTCTCCCAGAGCCTGA
  GCCACCAAAGCACAAGGCAGAAAGGAGTGCCTGCACAGACAAGGAGTTGCGGAACCTTGCCTCCCGGCTG
  AAGGATTGGTTTGGAGCTCTCCACGAGGATGCGAACAGAGTCATCAAGCCCACCAGCTCCAACACAGCCC
  AAGGCAGGTTTGACACTAGCATCCTGCCCATCTGCAAGGACTCCCTGGGCTGGATGTTCAACAAGTTGGA
  CATGAACTATGACCTCCTGCTTGACCCTTCAGAGATCAATGCCATCTACCTGGATAAGTACGAGCCCTGT
  ATCAAGCCTCTTTTCAACTCGTGTGACTCCTTCAAGGATGGCAAGCTTTCTAACAATGAGTGGTGCTACT
  GCTTCCAGAAGCCTGGAGGTCTCCCTTGCCAGAATGAAATGAACAGAATTCAGAAGCTGAGTAAGGGGAA
  AAGCCTGTTGGGGGCCTTCATACCTCGGTGTAATGAGGAGGGCTATTACAAAGCCACACAGTGCCACGGC
  AGCACGGGGCAGTGCTGGTGTGTGGACAAATATGGGAATGAGTTGGCTGGCTCCAGGAAACAGGGTGCTG
  TGAGCTGTGAAGAGGAGCAGGAAACCTCAGGGGATTTTGGCAGTGGTGGGTCCGTGGTCCTGCTGGATGA
  CCTAGAATATGAACGGGAGCTGGGACCAAAGGACAAAGAGGGGAAGCTGAGGGTGCACACCCGAGCCGTG
  ACAGAGGATGATGAGGATGAGGATGATGACAAAGAGGATGAGGTCGGGTACATATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205296 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAIAVLAAAAAAWCFLQVESRHLDALAGGAGPNHGNFLDNDQWLSTVSQYDRDKYWNRFRDDDYFRNWN
  PNKPFDKALDPSKDPCLKVKCSPHKVCVTQDYQTALCVSRKHLLPRQKKGNVAQKHWVGPSNLVKCKPCP
  VAQSAMVCGSDGHSYTSKCKLEFHACSTGKSLATLCDGPCPCLPEPEPPKHKAERSACTDKELRNLASRL
  KDWFGALHEDANRVIKPTSSNTAQGRFDTSILPICKDSLGWMFNKLDMNYDLLLDPSEINAIYLDKYEPC
  IKPLFNSCDSFKDGKLSNNEWCYCFQKPGGLPCQNEMNRIQKLSKGKSLLGAFIPRCNEEGYYKATQCHG
  STGQCWCVDKYGNELAGSRKQGAVSCEEEQETSGDFGSGGSVVLLDDLEYERELGPKDKEGKLRVHTRAV
  TEDDEDEDDDKEDEVGYIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004598
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004598.4
• RefSeq Size: 4841 bp
• RefSeq ORF: 1320 bp
• Locus ID: 6695
• UniProt ID: Q08629
• Cytogenetics: 5q31.2
• Domains: thyroglobulin_1, kazal
• Protein Families: Secreted Protein
• MW: 49.1 kDa
• Gene Summary: This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]