Parvin alpha (PARVA) (NM_018222) Human Tagged ORF Clone

CAT#: RC205086

PARVA (Myc-DDK-tagged)-Human parvin, alpha (PARVA)

Product Images

Circular map for RC205086

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PARVA (Cat# RC205086, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARVA(Cat# TA505991). Positive lysates LY413202 (100ug) and LC413202 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413202) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205086 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PARVA protein (Cat# TP305086). The protein was produced from HEK293T cells transfected with PARVA cDNA clone (Cat# RC205086) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parvin alpha
• Synonyms: CH-ILKBP; MXRA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205086 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTCCCCGCAGAAGTCGCCTTCTGTCCCCAAGTCTCCCACTCCCAAGTCGCCCCCGTCCCGCA
  AGAAAGATGATTCCTTCTTGGGGAAACTCGGAGGGACCCTGGCCCGGAGGAAGAAAGCCAAGGAGGTGTC
  CGAGCTGCAGGAGGAGGGAATGAACGCCATCAACCTGCCCCTCAGCCCAATTCCCTTTGAGCTGGACCCC
  GAGGACACGATGCTGGAGGAGAATGAGGTGCGAACAATGGTGGATCCAAACTCACGCAGTGACCCCAAGC
  TTCAAGAACTGATGAAGGTATTAATTGACTGGATTAATGATGTGTTGGTTGGAGAAAGAATCATTGTGAA
  AGACCTAGCTGAAGATTTGTATGATGGACAAGTCCTGCAGAAGCTTTTCGAGAAACTGGAGAGTGAGAAG
  CTAAATGTGGCTGAGGTCACCCAGTCAGAGATTGCTCAGAAGCAAAAACTGCAGACTGTCCTGGAGAAGA
  TCAATGAAACCCTGAAACTTCCTCCCAGGAGCATCAAGTGGAATGTGGATTCTGTTCATGCCAAGAGCCT
  GGTGGCCATCTTACACCTGCTCGTTGCTCTGTCTCAGTATTTCCGCGCACCAATTCGACTCCCAGACCAT
  GTTTCCATCCAAGTGGTTGTGGTCCAGAAACGAGAAGGAATCCTCCAGTCTCGGCAAATCCAAGAGGAAA
  TAACTGGTAACACAGAGGCTCTTTCCGGGAGGCATGAACGTGATGCCTTTGACACCTTGTTCGACCATGC
  CCCAGACAAGCTGAATGTGGTGAAAAAGACACTCATCACTTTCGTGAACAAGCACCTGAATAAACTGAAC
  CTGGAGGTCACAGAACTGGAAACCCAGTTTGCAGATGGGGTGTACCTGGTGCTGCTCATGGGGCTCCTGG
  AGGGCTACTTTGTGCCCCTGCACAGCTTCTTCCTGACCCCGGACAGCTTTGAACAGAAGGTCTTGAATGT
  CTCCTTTGCCTTTGAGCTCATGCAAGATGGAGGGTTGGAAAAGCCAAAACCGCGGCCAGAAGACATAGTC
  AACTGTGACCTGAAATCTACACTACGAGTGTTGTACAACCTCTTCACCAAGTACCGTAACGTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205086 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATSPQKSPSVPKSPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSELQEEGMNAINLPLSPIPFELDP
  EDTMLEENEVRTMVDPNSRSDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLESEK
  LNVAEVTQSEIAQKQKLQTVLEKINETLKLPPRSIKWNVDSVHAKSLVAILHLLVALSQYFRAPIRLPDH
  VSIQVVVVQKREGILQSRQIQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLN
  LEVTELETQFADGVYLVLLMGLLEGYFVPLHSFFLTPDSFEQKVLNVSFAFELMQDGGLEKPKPRPEDIV
  NCDLKSTLRVLYNLFTKYRNVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018222
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018222.5
• RefSeq Size: 8719 bp
• RefSeq ORF: 1119 bp
• Locus ID: 55742
• UniProt ID: Q9NVD7
• Cytogenetics: 11p15.3
• Domains: CH
• Protein Pathways: Focal adhesion
• MW: 42.2 kDa
• Gene Summary: This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq, Dec 2010]