RBM14 (NM_006328) Human Tagged ORF Clone

CAT#: RC201739

RBM14 (Myc-DDK-tagged)-Human RNA binding motif protein 14 (RBM14), transcript variant 1

Product Images

Circular map for RC201739

• 

  Western blot validation of overexpression lysate (Cat# LY416679) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201739 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBM14
• Synonyms: COAA; PSP2; SIP; SYTIP1; TMEM137
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201739 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATATTCGTGGGCAACGTCGACGGGGCGGATACGACTCCGGAGGAGCTGGCAGCCCTCTTTGCGC
  CCTACGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTCGCCTTCGTGCACATGCGCGAGAACGCGGG
  CGCGCTGCGCGCCATCGAAGCCCTGCACGGCCACGAGCTGCGGCCGGGGCGCGCGCTCGTGGTGGAGATG
  TCGCGCCCAAGGCCTCTTAATACTTGGAAGATTTTCGTGGGCAATGTGTCGGCTGCATGCACGAGCCAGG
  AACTGCGCAGCCTCTTCGAGCGCCGCGGACGCGTCATCGAGTGTGACGTGGTGAAAGACTACGCGTTTGT
  TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCAATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG
  CGCATCAACGTGGAACTCTCCACCAAGGGTCAGAAGAAGGGGCCTGGCCTGGCTGTCCAGTCTGGGGACA
  AGACCAAGAAACCAGGGGCTGGGGATACGGCCTTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA
  CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCCTTCTTT
  GGTCGCGACCGCAGCCCTCTGCGCCGTTCACCTCCCCGAGCCTCTTATGTGGCTCCTCTGACGGCCCAGC
  CAGCTACCTACCGGGCCCAGCCGTCCGTGTCACTGGGAGCTGCCTACAGGGCCCAGCCTTCTGCCTCTTT
  GGGTGTTGGCTATCGGACTCAGCCCATGACAGCCCAGGCAGCCTCTTACCGCGCTCAGCCCTCTGTCTCC
  CTTGGGGCACCATACAGGGGCCAGCTGGCTAGTCCTAGCTCCCAGTCTGCTGCAGCTTCTTCACTCGGCC
  CATATGGTGGAGCCCAGCCCTCAGCCTCGGCCCTTTCCTCCTATGGGGGTCAGGCAGCTGCAGCTTCTTC
  GCTCAACTCCTATGGGGCTCAGGGTTCCTCCCTTGCCTCCTATGGTAACCAGCCATCCTCTTACGGCGCC
  CAGGCTGCCTCTTCCTATGGGGTTCGTGCAGCTGCTTCTTCCTACAACACCCAGGGAGCAGCTTCCTCCT
  TAGGCTCCTACGGGGCTCAGGCAGCCTCCTATGGGGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC
  CCAGGCAGCTTCATATAATGCCCAGCCCTCGGCCTCTTACAATGCCCAGTCTGCCCCATATGCTGCACAG
  CAGGCTGCTTCCTACTCTTCCCAACCTGCTGCCTATGTGGCACAGCCAGCCACAGCTGCTGCCTATGCCA
  GCCAGCCAGCAGCCTACGCCGCACAAGCCACTACCCCAATGGCTGGCTCCTATGGGGCCCAGCCGGTTGT
  GCAGACCCAGCTGAATAGTTACGGGGCCCAAGCATCAATGGGCCTTTCAGGCTCCTATGGGGCTCAGTCG
  GCTGCTGCGGCCACTGGCTCCTATGGTGCCGCAGCAGCCTACGGGGCCCAACCTTCTGCCACCCTGGCAG
  CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC
  TGCCTCCTACCGCGGCCAGCCAGGCAATGCCTACGATGGGGCAGGTCAGCCGTCTGCAGCCTACCTGTCC
  ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCGCCGCCCTATGAGCGTACCCGCCTCTCCCCAC
  CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCGAAAAGGTATGGTTCCGACCGGCG
  TTTAGCCGAGCTCTCTGATTACCGCCGTTTATCAGAGTCGCAGCTTTCGTTCCGCCGCTCGCCGACAAAG
  TCCTCGCTGGATTACCGTCGCCTGCCCGATGCCCATTCCGATTACGCACGCTATTCGGGCTCCTATAATG
  ATTACCTGCGGGCGGCTCAGATGCACTCTGGCTACCAGCGCCGCATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201739 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGALRAIEALHGHELRPGRALVVEM
  SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK
  RINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF
  GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVS
  LGAPYRGQLASPSSQSAAASSLGPYGGAQPSASALSSYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA
  QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYNAQSAPYAAQ
  QAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQS
  AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLS
  MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTK
  SSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006328
• ORF Size: 2007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006328.4
• RefSeq Size: 5415 bp
• RefSeq ORF: 2010 bp
• Locus ID: 10432
• UniProt ID: Q96PK6
• Cytogenetics: 11q13.2
• Domains: RRM
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 69.5 kDa
• Gene Summary: This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011]