OTP (NM_032109) Human Tagged ORF Clone
CAT#: RC200982
OTP (Myc-DDK-tagged)-Human orthopedia homeobox (OTP)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_032109" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | OTP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200982 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCTCATGCCGACCTCCTGGACGCCAGGCTAGGTATGAAAGATGCCGCCGAGCTTCTGGGCCACC GGGAGGCGGTGAAGTGTAGGCTGGGCGTGGGGGGCTCCGACCCCGGGGGCCATCCGGGGGACCTGGCGCC CAACTCTGACCCAGTGGAGGGAGCCACTCTGCTGCCCGGGGAGGACATCACCACAGTGGGCTCTACTCCG GCCTCGCTGGCGGTGAGCGCCAAAGACCCGGACAAGCAGCCCGGGCCCCAGGGCGGCCCGAACCCCAGCC AAGCCGGCCAGCAGCAGGGCCAACAGAAGCAGAAGCGCCACCGGACGCGCTTCACCCCCGCACAGCTCAA CGAGTTGGAGAGGAGCTTCGCCAAGACTCACTACCCCGACATCTTTATGCGTGAGGAGCTGGCACTGCGT ATCGGGCTGACCGAGTCCCGAGTGCAGGTCTGGTTCCAGAACCGACGCGCCAAGTGGAAGAAGCGCAAGA AGACGACCAACGTGTTCCGTGCGCCCGGCACACTGCTGCCCACGCCAGGCCTGCCTCAGTTCCCGTCGGC TGCCGCCGCCGCTGCCGCCGCCATGGGCGACAGCCTGTGCTCTTTCCACGCCAACGACACCCGCTGGGCG GCGGCCGCCATGCCTGGCGTGTCACAGCTGCCTCTGCCGCCGGCGCTGGGCAGGCAGCAGGCCATGGCGC AGTCGCTGTCCCAGTGCAGCCTGGCGGCCGGTCCGCCGCCCAACTCCATGGGCCTGTCCAACAGCCTGGC GGGTTCCAACGGCGCGGGGCTGCAGTCGCACCTCTACCAGCCCGCCTTCCCCGGCATGGTGCCCGCCTCC CTCCCCGGCCCCAGCAACGTCTCCGGTTCGCCCCAGCTCTGCAGCTCCCCGGACAGCAGCGACGTGTGGC GGGGCACCAGCATCGCCTCCCTCCGCCGCAAGGCGCTAGAGCACACAGTCTCTATGAGCTTCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200982 protein sequence
Red=Cloning site Green=Tags(s) MLSHADLLDARLGMKDAAELLGHREAVKCRLGVGGSDPGGHPGDLAPNSDPVEGATLLPGEDITTVGSTP ASLAVSAKDPDKQPGPQGGPNPSQAGQQQGQQKQKRHRTRFTPAQLNELERSFAKTHYPDIFMREELALR IGLTESRVQVWFQNRRAKWKKRKKTTNVFRAPGTLLPTPGLPQFPSAAAAAAAAMGDSLCSFHANDTRWA AAAMPGVSQLPLPPALGRQQAMAQSLSQCSLAAGPPPNSMGLSNSLAGSNGAGLQSHLYQPAFPGMVPAS LPGPSNVSGSPQLCSSPDSSDVWRGTSIASLRRKALEHTVSMSFT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032109 |
ORF Size | 975 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032109.1 |
RefSeq Size | 2702 bp |
RefSeq ORF | 978 bp |
Locus ID | 23440 |
UniProt ID | Q5XKR4 |
Cytogenetics | 5q14.1 |
Protein Families | Transcription Factors |
MW | 34.2 kDa |
Gene Summary | This gene encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200982L3 | Lenti ORF clone of Human orthopedia homeobox (OTP), Myc-DDK-tagged |
USD 750.00 |
|
RC200982L4 | Lenti ORF clone of Human orthopedia homeobox (OTP), mGFP tagged |
USD 750.00 |
|
RG200982 | OTP (tGFP-tagged) - Human orthopedia homeobox (OTP) |
USD 650.00 |
|
SC126250 | OTP (untagged)-Human orthopedia homeobox (OTP) |
USD 450.00 |
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