Dhx34 (NM_001285931) Mouse Tagged ORF Clone

CAT#: MR231621

Dhx34 (myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 34 (Dhx34), transcript variant 1

Product Images

Circular map for MR231621

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhx34
• Synonyms: 1200013B07Rik; 1810012L18Rik; Ddx34; mKIAA0134
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231621 representing NM_001285931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCTCCCAGGACAAGGGAGGGGAGGGGTCACCGAGACCGAGACCACCATCGAGCTCCCAGGGAGG
  AAGAGGCCCCGGAGAAATGGGACTGGAATTGTCCAGAGACTCGTTGTCTCTTGGAAGATGTATTCTTCCG
  CGATGAGGATTATATCCGTCGTGGTTCAGAGGAGTGTCAGAAGTTCTGGGCTTTCTTTGAACGCCTGCAG
  AGATTCCAGCACCTCAAGACCTCCCAGAAGAAGGAGAAGGACCCTGGCATGCCCAAACATGGCATCGCTG
  CTTTAGCTGACCTACCTCTCACTTACGACCCACGCTACCGCATCAACCTCTCCATTCTCAGCCCAGACAC
  TCGGGGTCGTCATGGGCCCGGCAGGGGGCTGCCGCCCGAGAGAGTGTCCGAGTTCCGCCGTGCATTGCTT
  CACTACCTAGACTTCCAGCAGAAGCAGGCTTTTGGGAGGCTCGTCAAACTGCAGCGTGAGCGAGCGGCAC
  TTCCCATCGCCCAGTATGGGAACCGCATCCTGCAGACTCTCAAGGAACACCAGGTGGTGGTGGTGGCCGG
  GGACACAGGCTGTGGCAAGTCTACTCAGGTGCCCCAGTACTTGCTGGCCGCCGGCTTCAGTCACGTGGCA
  TGCACTCAGCCCCGGAGAATCGCCTGCATCTCACTGGCCAAGCGCGTTGGCTTCGAGAGCCTCAGTCAGT
  ATGGCTCCCAGGTTGGCTACCAGATCCGCTTTGAAAGCACTCGGTCGGCAGCCACCAAGATCGTGTTCCT
  GACGGTGGGGCTGCTCCTACGCCAGATCCAGCGTGAGCCCAGCCTGCCCCAGTACCAGGTCCTGATCGTG
  GACGAGGTCCACGAGCGACACCTGCACAATGACTTCCTGCTGGGTGTCCTGCAGCGCCTGCTGCCCCAGC
  GGCCTGACCTCAAGGTCATCCTCATGTCGGCCACCATCAACATCTCTCTCTTCTCCAGCTACTTCAGCCA
  TGCTCCGGTGGTGCAGGTGCCTGGGAGGCTCTTCCCCATCACGGTAGTCTACCAGCCGCAGGAGGCAGAC
  CAGACAGCATCCAAGTCAGAGAAGCTGGACCCACGGCCCTTCCTGAGGGTGCTGGAGGCCATCGACAATA
  AGTATCCCCCCGAGGAGCGGGGGGACCTTCTTGTCTTCCTCAGTGGCATGGCGGAGATCACCACGGTGCT
  GGACGCAGCCCAGGCTTATGCCAGCCTCACCCAGCGCTGGGTGGTGCTGCCGCTGCACAGCGCCCTCTCT
  GTGTCTGACCAGGACAAGGTGTTCGATGTGGCTCCAGCTGGAGTCCGGAAGTGCATCCTGTCCACCAACA
  TTGCCGAGACCTCAGTCACGATCGATGGCATCCGCTTCGTGGTGGATTCTGGGAAGGTGAAGGAGATGAG
  CTACGACCCACAGGCCAAACTGCAGCGGCTGCAGGAATTCTGGATCAGCCAGGCCAGTGCTGAGCAGCGC
  AAGGGCCGAGCGGGCCGCACGGGCCCCGGCGTCTGCTACCGTCTCTATGCCGAGTCTGACTATGATGCCT
  TTGCGCCGTACCCAGTCCCGGAAATCCGCAGGGTGGCCCTGGATGCACTGGTGCTTCAGATGAAGAGTAT
  GAGTGTGGGGGACCCACGCACCTTCCCCTTCATCGAGCCCCCTCCACCTGCCAGCGTGGAAACCGCTATC
  CTCTATCTCCAAGAGCAGGGGGCCCTGGACAGCTCGGAGGCCCTCACCCCCATCGGGTCCCTGCTGGCCC
  AGCTGCCTGTAGATGTTGTAATTGGGAAGATGCTGATCCTCGGCTCCATGTTCAGCCTGGCAGAGCCTGT
  GCTCACCATTGCTGCTGCCCTCAGCGTCCAGTCACCATTCACCCGCAGTGCCCAGAGCAACCTGGACTGT
  GCCACTGCCCGGAGACCGCTGGAAAGTGACCAGGGTGACCCCTTCACACTCTTCAATGTCTTCAATGCCT
  GGGTGCAGGTGAAGTCTGAGCGGAGCGGAAACTCTCGAAAGTGGTGCCGCCGCCGGGGTGTGGAGGAGCA
  CCGGCTGTATGAGATGGCCAACCTGCGGCGCCAGTTCAAGGAGCTGTTGGAGGATCATGGATTGCTGAGT
  GGGGCCCAGGTAGTGGCCCCAGGGGACAGCTACAGTCGGCTGCAGCAGCGCCGAGAGCGCAGGGCCCTGC
  ACCAGCTGAAGCGACAGCATGAGGAGGGTGGGGGACGCAGGCGCAAGGTGCTGAGGCTGCAGGAGGATGG
  CTGCTCCAGCGATGAAGAGGACCGAGAGGGCTCTACCTCCCAGAGGGCTGACAGCGTTGACATCCAGGAT
  GTGAAATTCAAGCTTCGGCACAACCTGGAGCAGCTGCAGGCAGCTGCCAGCTCAGCCCAGGACCTGACCC
  GAGACCAACTGGCCCTGCTCAAGCTGGTGCTCGGGCGGGGGCTGTACCCACAGCTGGCTGTGCCCGATGC
  GTTCAACAGTGGCCGCAAGGACTCAGACCAGATTTTCCACACGCAGGCCAAGCAGGGCACCGTGCTGCAC
  CCCACCTGTGTCTTTGCCAACAGCCCTGAGGTATTACACACACAGGGGCAGGAGGCCTCAGGCCAAGAAG
  GGAGCCAAGATGGCAGGGACCAAATGAGCTGCAAGCATCAGCTGCTGGCCTTCGTCTCCCTACTGGAGAC
  CAACAAGCCATACCTGGTGAACTGCGTCAGAATCCCGGCCCTCCAGTCCCTCCTGCTGTTCAGCCGCTCC
  ATTGACACCAACGGTGACTGCTCCCGCCTGGTGGCTGACGGTTGGCTAGAGCTGCAGCTCGCAGATAGCG
  AGAGTGCCGTCCGGCTCCTGGCAACATCCCTGCGGCTCCGTGTCCACTGGGAAAGTGCCCTGGACCGGCA
  GCTGGCCCGCCAGGCCCAGCGACGGAAGCTAGAGCAGGAGGAAGACATGGGGTCTCCAGCAGTCAGCCCC
  CAGGAGGTGGCTGCTCTGAGCAGGGAGCTGCTGCAGTTCATGGTGGCCAAGGTTCCCTACCGCCTCCGAA
  GGCTCACTGGTCTAGAAGCCCAGAACCTCTACGTGGGACCCCAGACCATCACGACCGCCCCTAGTCTGCC
  CGGCCTCTTTGGAAATTCTACCCTGTCTCCACACCCCACCAAGGGGGGCTACGCTGTCTCTGATTATCTC
  ACCTACAACTGCCTCACGAGTGACACTGACCTGTACAGTGACTGTCTGCGTTCCTTCTGGACCTGCCCAC
  ACTGTGGCCTGCACATGCCCTTCACACCCTTGGAGCGCATTGCTCATGAGAACACCTGCCCTGAGGCGCC
  AGGCGATGATCCAGGGTCTGAGGAGGCTGCTCCTGCACCACCACAGAAGACATCAGCCCTGCAGAGGCCC
  TACCACTGCCAGGTGTGCGGGCAGGACTTCCTCTTTACCCCAACTGAGGTACTAAGACATCGAAGGCAGC
  ATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231621 representing NM_001285931
  Red=Cloning site Green=Tags(s)
  
  MPPPRTREGRGHRDRDHHRAPREEEAPEKWDWNCPETRCLLEDVFFRDEDYIRRGSEECQKFWAFFERLQ
  RFQHLKTSQKKEKDPGMPKHGIAALADLPLTYDPRYRINLSILSPDTRGRHGPGRGLPPERVSEFRRALL
  HYLDFQQKQAFGRLVKLQRERAALPIAQYGNRILQTLKEHQVVVVAGDTGCGKSTQVPQYLLAAGFSHVA
  CTQPRRIACISLAKRVGFESLSQYGSQVGYQIRFESTRSAATKIVFLTVGLLLRQIQREPSLPQYQVLIV
  DEVHERHLHNDFLLGVLQRLLPQRPDLKVILMSATINISLFSSYFSHAPVVQVPGRLFPITVVYQPQEAD
  QTASKSEKLDPRPFLRVLEAIDNKYPPEERGDLLVFLSGMAEITTVLDAAQAYASLTQRWVVLPLHSALS
  VSDQDKVFDVAPAGVRKCILSTNIAETSVTIDGIRFVVDSGKVKEMSYDPQAKLQRLQEFWISQASAEQR
  KGRAGRTGPGVCYRLYAESDYDAFAPYPVPEIRRVALDALVLQMKSMSVGDPRTFPFIEPPPPASVETAI
  LYLQEQGALDSSEALTPIGSLLAQLPVDVVIGKMLILGSMFSLAEPVLTIAAALSVQSPFTRSAQSNLDC
  ATARRPLESDQGDPFTLFNVFNAWVQVKSERSGNSRKWCRRRGVEEHRLYEMANLRRQFKELLEDHGLLS
  GAQVVAPGDSYSRLQQRRERRALHQLKRQHEEGGGRRRKVLRLQEDGCSSDEEDREGSTSQRADSVDIQD
  VKFKLRHNLEQLQAAASSAQDLTRDQLALLKLVLGRGLYPQLAVPDAFNSGRKDSDQIFHTQAKQGTVLH
  PTCVFANSPEVLHTQGQEASGQEGSQDGRDQMSCKHQLLAFVSLLETNKPYLVNCVRIPALQSLLLFSRS
  IDTNGDCSRLVADGWLELQLADSESAVRLLATSLRLRVHWESALDRQLARQAQRRKLEQEEDMGSPAVSP
  QEVAALSRELLQFMVAKVPYRLRRLTGLEAQNLYVGPQTITTAPSLPGLFGNSTLSPHPTKGGYAVSDYL
  TYNCLTSDTDLYSDCLRSFWTCPHCGLHMPFTPLERIAHENTCPEAPGDDPGSEEAAPAPPQKTSALQRP
  YHCQVCGQDFLFTPTEVLRHRRQHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285931
• ORF Size: 3435 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285931.1, NP_001272860.1
• RefSeq Size: 4310 bp
• RefSeq ORF: 3438 bp
• Locus ID: 71723
• UniProt ID: Q9DBV3
• Cytogenetics: 7 A2
• MW: 128.6 kDa
• Gene Summary: Probable ATP-binding RNA helicase.[UniProtKB/Swiss-Prot Function]