Rabep1 (NM_001291143) Mouse Tagged ORF Clone
CAT#: MR231129
- TrueORF®
Rabep1 (myc-DDK-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1), transcript variant 4
"NM_001291143" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rabep1 |
Synonyms | Rab5ep; rabaptin-5; rabaptin-5alpha |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231129 representing NM_001291143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACATTAAGGCAATTGCCACAGTCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAA GGCAATGGAGAGAAGAAGTTGCTTCACTTCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCA GTTTCACCTGAGGCTGGAGCAGGAGCGAGCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATA GCTGACTTAAGAAGAAGGTTGTCTGAAGGTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCC AAGAGGATGCTGAAAAGCTTCGTTCTGTCGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAA ACTGACAGAAGCTGAAGACAAGATTAAAGAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTG GAGGCCGAGAAGTCTTGCAGGACTGACCTGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGC TACAGGAAGATGCTGAGAAATTGAGGAAAGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACA ACAACACAACCAGTTAAAGCATACATGGCAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTG ATGAGAGACATGCAGAGAATGGAGATTGTGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGA AGAAAGACCAGGAGGAGGATGAACAACAAAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGA AGAAGAAGTAAAAATACCAGTAGTGTGTGCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAA GAGGAGCATTTAGACAGCACCCATGGGTCAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAG ATCCATTTAGTAAATCGGACAATGACATGTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTT GGGAACCTCAAGCTCATTGCAATCCAAAGCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTG GATGAGTCGGATTTTGGACCACTGGTTGGAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTG GGTCACTGCAGATGCCAAGTGGATTTATGTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACC TGAGCAGGAGGAGACAGCATCCCTCCTCTCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCC AGTGGTTACCGCTTAGTGAGTGAGACGGAATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATA AGCTTGGTAGACGTTGTGATATGTGTTCCAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGC TGAAACAAGAGACCAGGTGAAAAAACTGCAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACG ATGAAAGAGAAGCAGGAGCTGGAGGACTTCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTG CACTTGTTTTAAGAGCCCAAGCCTCTGAGGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAA GAGGGACGTTCAGGAGCAGATGGCGGTGCTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGG TTACAGAAAGACAACGACAGCCTCCAGGGGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACT TCATTCTTCCAGACACTGTGGAGGTGCTCCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGT GCGCACAGCAGCAGACCACATGGAAGAGAAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAA GCGGAACAGTGTTTAAAAGAAAACCTGGAAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAA TAGCTTCCATTTCTAGTCTAAAAGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTAC ATTAAGAGAGAAGTCGCAACAACTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAG AAAGAAACTGCTGCTAAGGCTACTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGC AGACAGAATTAGATGTCAGTGAACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCA GTTGGAGAGGATCCGGCAAGCAGACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACA GACATTAACCAGCTCCCTGAGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231129 representing NM_001291143
Red=Cloning site Green=Tags(s) MENIKAIATVSENTKQEAIDEVKRQWREEVASLQAIMKETVRDYEHQFHLRLEQERAQWAQYRESAEREI ADLRRRLSEGQEEENLENEMKKAQEDAEKLRSVVMPMEKEIAALKDKLTEAEDKIKELEASKVKELNHYL EAEKSCRTDLEMYVAVLNTQKSVLQEDAEKLRKELHEVCHLLEQERQQHNQLKHTWQKANDQFLESQRLL MRDMQRMEIVLTSEQLRQVEELKKKDQEEDEQQRVNKRKDNKKTDTEEEVKIPVVCALTQEESSTPLSNE EEHLDSTHGSVHSLDADLMLPSGDPFSKSDNDMFKDGLRRAQSTDSLGTSSSLQSKALGYNYKAKSAGNL DESDFGPLVGADSVSENFDTVSLGSLQMPSGFMLTKDQERAIKAMTPEQEETASLLSSVTQGMESAYVSP SGYRLVSETEWNLLQKEVHNAGNKLGRRCDMCSNYEKQLQGIQIQEAETRDQVKKLQLMLRQANDQLEKT MKEKQELEDFLKQSAEDSSHQISALVLRAQASEVLLEELQQSFSQAKRDVQEQMAVLMQSREQVSEELVR LQKDNDSLQGKHSLHVSLQLAEDFILPDTVEVLRELVLKYRENIVHVRTAADHMEEKLKAEILFLKEQIQ AEQCLKENLEETLQLEIENCKEEIASISSLKAELERIKVEKGQLESTLREKSQQLESLQEMKVNLEEQLK KETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLT DINQLPET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001291143 |
ORF Size | 2334 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291143.1, NP_001278072.1 |
RefSeq Size | 5511 bp |
RefSeq ORF | 2337 bp |
Locus ID | 54189 |
UniProt ID | O35551 |
Cytogenetics | 11 43.21 cM |
MW | 90.3 kDa |
Gene Summary | Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228918 | Rabep1 (untagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1), transcript variant 4 |
USD 785.00 |
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