L3mbtl2 (NM_001289712) Mouse Tagged ORF Clone

CAT#: MR230958

L3mbtl2 (myc-DDK-tagged) - Mouse l(3)mbt-like 2 (Drosophila) (L3mbtl2), transcript variant 3

Product Images

Circular map for MR230958

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3mbtl2
• Synonyms: 4732493N06Rik; M4mbt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230958 representing NM_001289712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAACCGCGGGGCACTGAGGAGGCGCCATCTTCAGAGCCAATGGAAGAGGAGGAGGAAGATGACT
  TGGATCTCTTTGGGGGCTACGACAGCTTCCGGAGTTACAACAGCAGTGCGGGCAGCGAGAGCAGCTCCTA
  CTTGGAGGAGTCAAGTGAAGCAGAGAACGAGGACCGGGAAGCAGGGGAGCTGCCCACCTCACCTCTCCAT
  CTGTTCAGCTCTGCGAACAACCGCTCCTTGGATGGAAGTGGTTCTGAGCCAGCTGTCTGTGAGATGTGTG
  GTATCGTGGGCACAAGAGAAGCTTTCTTCTCAAAGACCAAGAGGTTCTGCAGCGTCTCGTGTTCCAGGAG
  CTACTCCTCCAACTCCAAGAAAGCCAGTATCCTGGCTAGACTACAGGGAAAGCCACCCACCAAGAAAGCC
  AAAGTCCTGCACAAGGCAGCCTGGTCTGCCAAAATTGGAGCCTTTCTCCATGCCCAGGGAACAGGACAAC
  TAGCAGATGGGACACCCACTGGACAAGATGCTCTGGTCCTGGGCTTCGACTGGGGCAAGTTCCTGAAGGA
  TCATAGTTACAAGGCTGCTCCTGTCAGCTGCTTTAAACATGTACCCCTCTATGACCAGTGGGAAGACGTC
  ATGAAGGGGATGAAGGTGGAAGTGCTCAACAGCGATGCTGTGCTCCCCAGCCGGGTGTACTGGATCGCCA
  CTGTCATCCAGGCAGCTGGGTACCGGGTGCTGCTCCGATATGAAGGCTTTGAAAATGACGCCAGTCATGA
  CTTCTGGTGCAACTTGGGAACTGTGGATGTCCACCCCATTGGGTGGTGTGCCATCAACAGCAAGATCCTG
  GTACCTCCACGGACCATCCATGCCAAGTTTACTGACTGGAAGAGCTACCTCATGAAGCGGTTGGTGGGTT
  CCAGGACACTTCCTGCAGACTTCCATATCAAGATGGTGGAAAGCATGAAGTACCCTTTCCGACAGGGCAT
  GCGCTTAGAGGTTGTAGACAAGACTCAGGTGTCACGTACCCGCATGGCCGTGGTGGACACAGTAATCGGG
  GGTCGCCTCCGGCTCCTCTATGAGGATGGTGACAGTGATGACGACTTCTGGTGCCATATGTGGAGTCCCC
  TGATCCACCCAGTGGGTTGGTCCCGGCGTGTTGGCCACGGCATCAAGATGTCAGACAGACGATGTGACAT
  GTCTCATCACCCCACCTTCCGGAAAATCTACTGTGATGCTGTACCTTACCTCTTCAAGAAGGTCCGCGCT
  GTCTACACAGAAGGTGGCTGGTTCGAGGAAGGAATGAAACTAGAGGCCATTGACCCTCTGAATCTGGGCA
  GTATCTGTGTAGCAACCATCTGCAAGGTGCTCTTGGATGGTTACCTGATGATCTGTGTGGATGGGGGGCC
  CTCCACGGATGGCTCCGATTGGTTCTGCTACCACGCCTCCTCCCATGCCATCTTCCCAGCCACCTTCTGC
  CAAAAGAATGACATTGAGCTCACACCCCCAAAAGGGTATGAGACACAGCCTTTTGCCTGGGAGACCTACT
  TAGAGAAGACCAAGTCAAAAGCTGCTCCAGCAAGACTCTTTAACATGGACTGCCCCAACCATGGCTTCAA
  GGTGGGCATGAAGCTGGAGGCTGTGGACCTGATGGAGCCCCGGCTCATCTGTGTGGCCACTGTGAAGCGG
  GTGGTGCATCGGCTCCTCAGCATCCACTTCGACGGCTGGGACAATGAGTATGATCAGTGGGTGGACTGCG
  AATCCCCGGACATCTACCCTGTCGGCTGGTGTGAGCTCACCGGCTACCAGCTCCAGCCGCCTGTGTCCGC
  AGAACCAAACACACCTCAGAAAGGCAAGGACACCACAAAGAAGAAGAAGAAACAGTTTGGGAAGAAAAGG
  AAAAGAATCCCATCAGCCAAGACTCGGCCCCTCAGACAGGGCTCCAAGAAACCCTTACTGGAGGACAACC
  TTGAGGCTTTGGGGGTCTCGGAACCAGTTCCTGATGACAGTCGCCCAGTCCACAGCTGCCTCTTCCCATT
  GAGAGCATCAAGCAGGAGAGGAACAACTGAGACTTCCCTGGCATCAGCCTGGACCCTAACTGAAGCCAAG
  CATGGAGCAGAGAGGAGGCCCGGCTTTACTGCTGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230958 representing NM_001289712
  Red=Cloning site Green=Tags(s)
  
  MEKPRGTEEAPSSEPMEEEEEDDLDLFGGYDSFRSYNSSAGSESSSYLEESSEAENEDREAGELPTSPLH
  LFSSANNRSLDGSGSEPAVCEMCGIVGTREAFFSKTKRFCSVSCSRSYSSNSKKASILARLQGKPPTKKA
  KVLHKAAWSAKIGAFLHAQGTGQLADGTPTGQDALVLGFDWGKFLKDHSYKAAPVSCFKHVPLYDQWEDV
  MKGMKVEVLNSDAVLPSRVYWIATVIQAAGYRVLLRYEGFENDASHDFWCNLGTVDVHPIGWCAINSKIL
  VPPRTIHAKFTDWKSYLMKRLVGSRTLPADFHIKMVESMKYPFRQGMRLEVVDKTQVSRTRMAVVDTVIG
  GRLRLLYEDGDSDDDFWCHMWSPLIHPVGWSRRVGHGIKMSDRRCDMSHHPTFRKIYCDAVPYLFKKVRA
  VYTEGGWFEEGMKLEAIDPLNLGSICVATICKVLLDGYLMICVDGGPSTDGSDWFCYHASSHAIFPATFC
  QKNDIELTPPKGYETQPFAWETYLEKTKSKAAPARLFNMDCPNHGFKVGMKLEAVDLMEPRLICVATVKR
  VVHRLLSIHFDGWDNEYDQWVDCESPDIYPVGWCELTGYQLQPPVSAEPNTPQKGKDTTKKKKKQFGKKR
  KRIPSAKTRPLRQGSKKPLLEDNLEALGVSEPVPDDSRPVHSCLFPLRASSRRGTTETSLASAWTLTEAK
  HGAERRPGFTAVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289712
• ORF Size: 2139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289712.1, NP_001276641.1
• RefSeq Size: 3435 bp
• RefSeq ORF: 2142 bp
• Locus ID: 214669
• Cytogenetics: 15 E1
• MW: 80.4 kDa
• Gene Summary: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27' (By similarity).[UniProtKB/Swiss-Prot Function]