Mapkap1 (NM_001290625) Mouse Tagged ORF Clone

CAT#: MR230361

Mapkap1 (myc-DDK-tagged) - Mouse mitogen-activated protein kinase associated protein 1 (Mapkap1), transcript variant 2

Product Images

Circular map for MR230361

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapkap1
• Synonyms: AI591529; D230039K05Rik; Sin1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230361 representing NM_001290625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCTTGGACAATCCAACTATCATTCTAGCTCATATTCGACAGTCACACGTGACCAGTGATGACA
  CAGGAATGTGTGAGATGGTTCTCATTGATCATGACGTTGACCTAGAGAAGACTCATCCTCCGTCAGTGCC
  TGGAGACAGTGGGTCAGAAGTTCAGGGAAGCAGTGGTGAGACGCAGGGCTACATATACGCCCAGTCTGTT
  GATATTACATCGAGCTGGGACTTTGGTATTAGAAGACGCTCAAACACAGCTCAAAGATTAGAACGACTCC
  GCAAAGAGAGACAAAACCAGATCAAATGCAAAAATATTCAGTGGAAAGAAAGAAATTCTAAACAATCAGC
  CCAGGAGTTAAAGTCACTGTTTGAAAAAAAATCCCTCAAAGAGAAGCCTCCAAGTTCAGGCAAGCAGTCC
  ATATTGTCTGTACGCCTGGAGCAGTGCCCTCTGCAGCTGAATAACCCCTTTAATGAGTACTCCAAGTTTG
  ATGGCAAGGGTCATGTGGGTACAACTGCAACTAAGAAGATCGACGTCTACCTCCCCCTGCACTCCAGCCA
  GGACAGACTGCTGCCAATGACCGTGGTGACCATGGCCAGCGCCAGGGTGCAGGACCTCATCGGGCTCATC
  TGTTGGCAGTACACGAGTGAAGGACGGGAGCCGAAGCTCAATGACAATGTCAGTGCCTACTGCCTGCATA
  TTGCTGAGGATGATGGGGAGGTCGACACGGATTTTCCGCCACTGGATTCCAATGAGCCCATTCATAAGTT
  TGGCTTCAGTACTTTGGCCCTGGTTGAAAAATACTCCTCTCCTGGTCTGACCTCCAAAGAGTCGCTCTTT
  GTTCGAATAAATGCTGCTCATGGATTCTCCCTTATCCAAGTAGACAACACAAAGGTCACCATGAAGGAGA
  TCTTGCTCAAGGCAGTGAAAAGAAGAAAAGGATCCCAGAAAATTTCAGGCCCTCAGTACCGCCTGGAGAA
  GCAGAGTGAGCCTAACATCGCTGTGGACCTGGAGAGCACGCTGGAGAGCCAGAACGCCTGGGAGTTCTGC
  CTGGTTCGCGAGAACAGTTCAAGGGCAGACGGAGTTTTCGAGGAGGATTCACAAATTGACATCGCTACAG
  TACAGGATATGCTTAGCAGCCACCATTACAAGTCATTCAAAGTCAGCATGATCCACAGACTGCGATTCAC
  GACGGATGTGCAGTTAGGTATCTCTGGAGACAAAGTAGAGATAGACCCTGTTACGAATCAGAAAGCCAGC
  ACTAAGTTTTGGATTAAGCAGAAACCCATCTCAATCGATTGTGACCTGCTCTGTGCCTGTGACCTTGCCG
  AGGAGAAGAGCCCCAGTCACGCGGTGTTTAAGCTCACGTATCTAAGCAGCCATGACTACAAGCACCTGTA
  CTTCGAGTCCGACGCAGCCACAGTCAGTGAGATCGTGCTCAAGGTTAACTACATCCTGGAATCACGAGCG
  AGCACTGCCCGAGCTGATTATCTTGCTCAAAAACAAAGAAAACTGAACAGGCGAACAAGCTTCAGCTTCC
  AGAAGGAGAAGAAATCAGGGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230361 representing NM_001290625
  Red=Cloning site Green=Tags(s)
  
  MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKTHPPSVPGDSGSEVQGSSGETQGYIYAQSV
  DITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPSSGKQS
  ILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLI
  CWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLF
  VRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKISGPQYRLEKQSEPNIAVDLESTLESQNAWEFC
  LVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKAS
  TKFWIKQKPISIDCDLLCACDLAEEKSPSHAVFKLTYLSSHDYKHLYFESDAATVSEIVLKVNYILESRA
  STARADYLAQKQRKLNRRTSFSFQKEKKSGQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290625
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290625.1, NP_001277554.1
• RefSeq Size: 3126 bp
• RefSeq ORF: 1569 bp
• Locus ID: 227743
• UniProt ID: Q8BKH7
• Cytogenetics: 2 B
• MW: 59 kDa
• Gene Summary: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.[UniProtKB/Swiss-Prot Function]