Steap2 (NM_001285470) Mouse Tagged ORF Clone

CAT#: MR230181

Steap2 (myc-DDK-tagged) - Mouse six transmembrane epithelial antigen of prostate 2 (Steap2), transcript variant 5

Product Images

Circular map for MR230181

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Steap2
• Synonyms: 4921538B17Rik; AI930049; AW045895; IPCA-1; IPCA1; PCANAP1; STAMP1; STMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230181 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCCATCTCTATGATGGGAAGCCCTAAGAGCCTGGAGACGTTTTTGCCTAATGGCATAAATGGTA
  TCAAAGACGCAAGGCAGGTCACCGTGGGGGTGATAGGAAGTGGGGATTTTGCCAAGTCTCTGACCATTCG
  GCTTATTAGATGTGGCTACCACGTGGTCATAGGAAGCAGAAATCCCAAGTTTGCATCAGAATTTTTTCCT
  CACGTGGTAGACGTCACCCACCATGAAGATGCTTTAACAAAAACAAATATAATATTCGTGGCTATCCATA
  GAGAACATTACACCTCCTTGTGGGACCTGAGACATCTGCTTGTGGGCAAAATCCTCATTGATGTGAGCAA
  CAACATGAGAGTAAACCAGTACCCAGAATCCAATGCAGAGTACCTGGCTTCATTATTCCCGGACTCCTTG
  ATTGTCAAAGGATTTAATGTGATCTCAGCTTGGGCACTTCAGCTAGGTCCCAAGGATGCCAGCCGCCAGG
  TTTATATATGCAGCAACAATATCCAAGCTCGACAGCAGGTTATCGAGCTCGCCCGCCAGCTGAATTTTAT
  TCCTGTTGACTTGGGATCTTTGTCGTCAGCCAAGGAGATTGAAAACTTACCTCTGCGACTGTTTACTCTC
  TGGAGGGGGCCAGTGGTAGTAGCCATAAGCTTGGCCACATTTTTCTTTCTTTATTCCTTTGTCAGAGATG
  TGATACATCCATATGCCAGAAACCAGCAGAGTGACTTTTACAAGATTCCCATTGAGATTGTGAACAAAAC
  CTTGCCGATCGTCGCCATCACCCTGCTGTCTCTGGTGTACCTGGCTGGCCTCCTGGCAGCTGCGTATCAG
  CTTTATTATGGCACTAAGTACCGCCGATTTCCCCCGTGGCTGGATACTTGGCTGCAGTGCAGGAAACAGC
  TGGGATTGCTGAGCTTCTTCTTTGCAGTTGTTCACGTAGCCTACAGCCTCTGCTTACCAATGAGGAGGTC
  GGAAAGATACCTGTTCCTCAACATGGCTTATCAGCAGGTTCATGCCAATATTGAGAACGCGTGGAACGAG
  GAGGAGGTCTGGAGGATTGAGATGTACATTTCCTTTGGCATCATGAGCCTGGGCTTGTTGTCCCTGCTGG
  CGGTCACTTCCATCCCATCAGTGAGCAACGCTTTGAACTGGAGAGAGTTCAGTTTCATCCAGTCTACGCT
  TGGCTACGTCGCCCTGCTCATCACGACCTTCCACGTGTTAATTTACGGATGGAAGCGTGCGTTTGCAGAA
  GAGTACTACCGCTTTTACACACCACCAAACTTCGTTCTTGCCCTCGTTTTGCCCTCCATTGTAATTCTGG
  GTAAGATGATATTACTCCTCCCATGCATAAGCCGAAAGCTAAAACGAATTAAAAAGGGCTGGGAAAAGAG
  CCAGTTTCTAGACGAAGGCATGGGAGGAGCGGTTCCTCATCTGTCCCCAGAGAGGGTCACAGTGATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230181 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESISMMGSPKSLETFLPNGINGIKDARQVTVGVIGSGDFAKSLTIRLIRCGYHVVIGSRNPKFASEFFP
  HVVDVTHHEDALTKTNIIFVAIHREHYTSLWDLRHLLVGKILIDVSNNMRVNQYPESNAEYLASLFPDSL
  IVKGFNVISAWALQLGPKDASRQVYICSNNIQARQQVIELARQLNFIPVDLGSLSSAKEIENLPLRLFTL
  WRGPVVVAISLATFFFLYSFVRDVIHPYARNQQSDFYKIPIEIVNKTLPIVAITLLSLVYLAGLLAAAYQ
  LYYGTKYRRFPPWLDTWLQCRKQLGLLSFFFAVVHVAYSLCLPMRRSERYLFLNMAYQQVHANIENAWNE
  EEVWRIEMYISFGIMSLGLLSLLAVTSIPSVSNALNWREFSFIQSTLGYVALLITTFHVLIYGWKRAFAE
  EYYRFYTPPNFVLALVLPSIVILGKMILLLPCISRKLKRIKKGWEKSQFLDEGMGGAVPHLSPERVTVM
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285470
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285470.1, NP_001272399.1
• RefSeq Size: 10215 bp
• RefSeq ORF: 1470 bp
• Locus ID: 74051
• UniProt ID: Q8BWB6
• Cytogenetics: 5 A1
• MW: 55.8 kDa
• Gene Summary: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.[UniProtKB/Swiss-Prot Function]