Rarb (NM_001289762) Mouse Tagged ORF Clone

CAT#: MR229704

Rarb (myc-DDK-tagged) - Mouse retinoic acid receptor, beta (Rarb), transcript variant beta4

Product Images

Circular map for MR229704

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rarb
• Synonyms: A830025K23; Hap; Nr1b2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229704 representing NM_001289762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAAAATGCCATCGAGACACAGAGTACCAGCTCTGAGGAGCTCGTCCCGAGCCCACCATCTCCACTTC
  CTCCTCCTCGGGTGTACAAGCCCTGCTTCGTTTGCCAGGACAAGTCATCGGGCTACCACTATGGCGTCAG
  TGCCTGCGAGGGGTGCAAGGGCTTTTTCCGCAGAAGTATTCAGAAGAACATGATCTACACTTGCCATCGA
  GATAAGAACTGCGTCATTAACAAGGTCACTAGGAACCGATGCCAGTACTGCCGCCTGCAGAAGTGCTTTG
  AAGTGGGCATGTCCAAAGAGTCTGTTAGGAATGACAGGAACAAGAAAAAGAAGGAGCCTTCAAAGCAGGA
  ATGCACAGAGAGCTATGAGATGACAGCGGAGCTAGACGACCTCACTGAGAAGATCCGGAAAGCCCACCAG
  GAAACCTTTCCCTCACTCTGCCAGCTGGGTAAATACACCACGAATTCCAGCGCTGACCACCGGGTCCGAT
  TGGACTTGGGCCTCTGGGACAAATTCAGTGAGCTGGCCACCAAGTGCATTATTAAGATCGTGGAGTTCGC
  CAAGCGTCTGCCGGGCTTCACAGGTCTGACCATCGCAGACCAGATCACCCTGCTCAAAGCCGCCTGCTTG
  GATATCTTGATTCTCAGAATTTGTACCAGGTATACCCCAGAGCAAGACACCATGACTTTCTCTGATGGCC
  TTACACTAAATCGAACTCAGATGCACAATGCTGGCTTCGGTCCTCTGACTGACCTTGTGTTCACCTTTGC
  CAACCAGCTCCTGCCTTTGGAAATGGATGACACAGAAACAGGCCTTCTCAGTGCCATCTGTTTAATCTGT
  GGAGACCGCCAGGACCTTGAGGAACCAACAAAAGTAGACAAGCTCCAAGAACCACTGCTGGAAGCACTAA
  AGATTTACATTAGAAAACGACGACCCAGCAAGCCTCACATGTTTCCAAAGATCTTAATGAAAATCACAGA
  TCTCCGCAGCATCAGCGCGAAAGGTGCCGAACGTGTAATTACCTTGAAAATGGAAATTCCTGGATCAATG
  CCACCTCTCATTCAGGAAATGCTGGAGAATTCTGAAGGACATGAACCCTTGACCCCAAGTTCAAGTGGGA
  ATATAGCAGAGCACAGTCCCAGCGTGTCCCCCAGCTCAGTGGAGAACAGTGGAGTCAGTCAGTCACCACT
  GCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229704 representing NM_001289762
  Red=Cloning site Green=Tags(s)
  
  LENAIETQSTSSEELVPSPPSPLPPPRVYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMIYTCHR
  DKNCVINKVTRNRCQYCRLQKCFEVGMSKESVRNDRNKKKKEPSKQECTESYEMTAELDDLTEKIRKAHQ
  ETFPSLCQLGKYTTNSSADHRVRLDLGLWDKFSELATKCIIKIVEFAKRLPGFTGLTIADQITLLKAACL
  DILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFTFANQLLPLEMDDTETGLLSAICLIC
  GDRQDLEEPTKVDKLQEPLLEALKIYIRKRRPSKPHMFPKILMKITDLRSISAKGAERVITLKMEIPGSM
  PPLIQEMLENSEGHEPLTPSSSGNIAEHSPSVSPSSVENSGVSQSPLLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289762
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289762.1, NP_001276691.1
• RefSeq Size: 2687 bp
• RefSeq ORF: 1200 bp
• Locus ID: 218772
• UniProt ID: P22605
• Cytogenetics: 14 7.08 cM
• MW: 45.3 kDa
• Gene Summary: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (By similarity). The RXRA/RARB heterodimer can act as a repressor on the DR1 element and as an activator on the DR5 element (By similarity). In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (PubMed:19389355).[UniProtKB/Swiss-Prot Function]