Kcnip4 (NM_001199245) Mouse Tagged ORF Clone

CAT#: MR228670

Kcnip4 (myc-DDK-tagged) - Mouse Kv channel interacting protein 4 (Kcnip4), transcript variant 6

Product Images

Circular map for MR228670

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnip4
• Synonyms: AV032399; Calp; Calp250; KChIP4; KChIP4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228670 representing NM_001199245
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACTCTAGAGTGGGAGTCGGAAGGACTGCAAACAGTGGGTATTGTTGTGATTATATGTGCGTCTC
  TGAAGCTGCTTCATTTGCTGGGACTGATTGATTTTTCGGAAGACAGCGTGGAAGATGAGCTGGAGATGGC
  TACTGTCAGGCATCGGCCTGAAGCCCTGGAGCTGCTGGAGGCCCAGAGCAAATTCACCAAGAAAGAGCTT
  CAGATTCTTTACAGAGGATTTAAGAATGAATGCCCCAGTGGTGTTGTTAATGAAGAAACTTTCAAGGAGA
  TTTACTCACAGTTCTTTCCACAGGGAGACTCCACCACATATGCACATTTTCTCTTCAATGCATTCGACAC
  GGACCACAATGGAGCTGTGAGCTTTGAGGATTTCATCAAAGGTCTTTCCATTTTGCTTCGAGGGACAGTA
  CAAGAAAAACTCAACTGGGCATTTAATTTGTATGACATAAACAAAGATGGCTACATCACTAAAGAAGAAA
  TGCTGGACATAATGAAAGCAATCTACGACATGATGGGGAAATGCACATACCCGGTCCTCAAGGAAGATGC
  TCCCCGACAGCATGTGGAGACGTTCTTCCAGAAGATGGACAAAAATAAAGATGGTGTCGTTACCATAGAT
  GAGTTCATTGAAAGTTGCCAAAAAGATGAAAACATAATGCGCTCCATGCAGCTCTTTGAAAATGTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228670 representing NM_001199245
  Red=Cloning site Green=Tags(s)
  
  MLTLEWESEGLQTVGIVVIICASLKLLHLLGLIDFSEDSVEDELEMATVRHRPEALELLEAQSKFTKKEL
  QILYRGFKNECPSGVVNEETFKEIYSQFFPQGDSTTYAHFLFNAFDTDHNGAVSFEDFIKGLSILLRGTV
  QEKLNWAFNLYDINKDGYITKEEMLDIMKAIYDMMGKCTYPVLKEDAPRQHVETFFQKMDKNKDGVVTID
  EFIESCQKDENIMRSMQLFENVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199245
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199245.1, NP_001186174.1
• RefSeq Size: 2256 bp
• RefSeq ORF: 702 bp
• Locus ID: 80334
• Cytogenetics: 5 B3
• MW: 27.4 kDa
• Gene Summary: Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3 (PubMed:19109250). Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.[UniProtKB/Swiss-Prot Function]