Suv39h2 (NM_022724) Mouse Tagged ORF Clone
CAT#: MR227389
- TrueORF®
Suv39h2 (Myc-DDK-tagged) - Mouse suppressor of variegation 3-9 homolog 2 (Drosophila) (Suv39h2), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_022724" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Suv39h2 |
Synonyms | 4930507K23Rik; AA536750; D030054H19Rik; D2Ertd544e; KMT1B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227389 representing NM_022724
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCAGGGCCAAGGCACGGGGCAGTGAGGCAGGAGCGCGGTGTCACCGGGCTCCAGGTCCGC CCCCGAGGCCCAAGGCCAGGCGAACGGCGAGACGCCGCCGCGCGGAGACCCTGACGGCGCGACGCTCGCG GCCGTCTGCGGGCGAGAGGCGCGCCGGCTCCCAGCGAGCGTGGTCCGGAGCTCCGCGGGCCGCGGTCTTT GGCGACGAGTGTGCACGAGGTGCCTTATTCAAGGCCTGGTGTGTGCCTTGCCTAGTTTCACTTGATACTC TCCAGGAATTATGTAGAAAAGAAAAGCTCACATGTAAATCGATTGGAATCACCAAAAGGAATCTAAACAA TTATGAGGTGGAGTACTTGTGTGACTACAAGGTAGCAAAGGGTGTGGAATATTATCTTGTAAAATGGAAA GGATGGCCAGATTCTACAAACACCTGGGAGCCCTTGAGAAACCTCAGGTGTCCACAGCTCCTGCGGCAGT TCTCTGATGACAAGAAGACTTACTTAGCTCAGGAAAGGAAATGCAAGGCTGTCAATTCAAAATCCTTGCA ACCTGCAATTGCTGAGTATATTGTACAGAAAGCTAAGCAAAGAATAGCTCTGCAGAGATGGCAAGATTAC CTCAACAGAAGAAAGAACCATAAGGGGATGATATTTGTTGAAAACACTGTTGACTTGGAGGGCCCACCTT TAGACTTCTACTACATTAACGAGTACAGGCCAGCTCCCGGGATCAGCATAAACAGTGAAGCCACCTTTGG ATGTTCATGTACAGACTGCTTCTTTGACAAGTGTTGTCCTGCTGAAGCTGGAGTTGTGTTGGCTTATAAT AAGAAGCAACAAATTAAAATCCAACCAGGCACTCCCATCTACGAATGCAACTCAAGGTGTCGATGTGGAC CTGAATGTCCCAATAGGATTGTACAAAAAGGCACACAATATTCACTGTGCATCTTTAGAACTAGCAATGG CTGTGGTTGGGGTGTAAAAACCCTTGTGAAGATTAAAAGAATGAGTTTTGTCATGGAATATGTTGGAGAG GTGATCACAAGTGAAGAGGCCGAGAGACGGGGACAGTTCTATGACAACAAAGGGATCACCTACCTCTTTG ACCTGGACTACGAGTCTGATGAGTTCACAGTGGATGCAGCTCGATATGGAAACGTATCCCATTTTGTGAA TCATAGTTGTGACCCAAATCTTCAGGTGTTTAGTGTTTTCATCGATAACCTTGATACTCGGCTGCCCAGG ATAGCATTGTTCTCTACAAGAACCATAAACGCTGGAGAAGAGCTGACTTTTGACTATCAAATGAAAGGTT CTGGAGAAGCATCTTCAGACTCCATTGACCACAGCCCTGCCAAAAAAAGGGTCAGAACCCAATGTAAATG TGGAGCCGAGACTTGCAGAGGTTACCTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227389 representing NM_022724
Red=Cloning site Green=Tags(s) MAAARAKARGSEAGARCHRAPGPPPRPKARRTARRRRAETLTARRSRPSAGERRAGSQRAWSGAPRAAVF GDECARGALFKAWCVPCLVSLDTLQELCRKEKLTCKSIGITKRNLNNYEVEYLCDYKVAKGVEYYLVKWK GWPDSTNTWEPLRNLRCPQLLRQFSDDKKTYLAQERKCKAVNSKSLQPAIAEYIVQKAKQRIALQRWQDY LNRRKNHKGMIFVENTVDLEGPPLDFYYINEYRPAPGISINSEATFGCSCTDCFFDKCCPAEAGVVLAYN KKQQIKIQPGTPIYECNSRCRCGPECPNRIVQKGTQYSLCIFRTSNGCGWGVKTLVKIKRMSFVMEYVGE VITSEEAERRGQFYDNKGITYLFDLDYESDEFTVDAARYGNVSHFVNHSCDPNLQVFSVFIDNLDTRLPR IALFSTRTINAGEELTFDYQMKGSGEASSDSIDHSPAKKRVRTQCKCGAETCRGYLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022724 |
ORF Size | 1431 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022724.4, NP_073561.2 |
RefSeq Size | 4282 bp |
RefSeq ORF | 1434 bp |
Locus ID | 64707 |
UniProt ID | Q9EQQ0 |
Cytogenetics | 2 1.95 cM |
MW | 54.5 kDa |
Gene Summary | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216497 | Suv39h2 (untagged) - Mouse suppressor of variegation 3-9 homolog 2 (Drosophila) (Suv39h2), transcript variant 1, (10ug) |
USD 732.00 |
|
MG227389 | Suv39h2 (tGFP-tagged) - Mouse suppressor of variegation 3-9 homolog 2 (Drosophila) (Suv39h2) transcript variant 1, (10ug) |
USD 886.00 |
|
MR227389L3 | Lenti ORF clone of Suv39h2 (Myc-DDK-tagged) - Mouse suppressor of variegation 3-9 homolog 2 (Drosophila) (Suv39h2), transcript variant 1 |
USD 986.00 |
|
MR227389L4 | Lenti ORF clone of Suv39h2 (mGFP-tagged) - Mouse suppressor of variegation 3-9 homolog 2 (Drosophila) (Suv39h2), transcript variant 1 |
USD 986.00 |
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