Dhx8 (NM_144831) Mouse Tagged ORF Clone

CAT#: MR226459

Dhx8 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8)

Product Images

Circular map for MR226459

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhx8
• Synonyms: Ddx8; mDEAH6; mKIAA4096
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226459 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAGCCGTGGCCGCCGCCGGGGTCCTAATGGGCTCGGAGCCCGGCCCGGCGGAAGAACTCGCTA
  AACTTGAGTACCTGTCTTTGGTGTCGAAGGTTTGCACGGAGCTGGACAATCACTTGGGCATCAACGACAA
  GGATCTGGCTGAATTTGTCATCAGTCTTGCTGAGAAAAATACTACCTTTGATACTTTTAAGGCTTCTCTG
  GTCAAAAATGGTGCAGAATTCACAGATTCTCTTATTAGCAACCTCCTGCGTCTCATACAGACCATGCGGC
  CTCCTGCAAAGCCTTCTACCAGTAAAGATCCGGTTGTTAAACCCAAAACCGAAAAAGAAAAGCTGAGGGA
  ACTCTTCCCCGTCCTCTGCCAGCCGGACAACCCTTCAGCTCGGACCATGTTGGATGAGGAAGATGTGAAA
  GTCGCTGTGGACGTCTTGAAAGAGCTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAAGAGAAACAAAGAG
  ACCCTGAGCACAGGGACAGGACAAAGAAGAAGAAGAGGAGTCGGAGCCGAGACCGAGATCGAGACCGAGA
  CCGAGACCGGGACCGGGACCGGGACCGGGACAGGGACCGTGACAAGGACCGAGAACGAGATAGAGATCGA
  GAACGAGACAGAGAGCGAGATAGAGAGAGAGACCATAAGCGGAGACACCGATCACGCTCTCGGTCACATT
  CTAGAACCCGGGAGAGGACTAAGGGGAAATCTAGATACAGATCTAGGAGCAGAAGTCAGAGTCCCTTCAA
  AGATCGGAAAGACCGAGAAAAGTATGGGGAGAGGAACTTGGACAGATGGCGGGATAAGCATGTGGACCGC
  CCTCCCCCGGAAGAGCCTGCCATTGGAGATATTTACAATGGCAAAGTGACCAGTATCATGCAGTTCGGTT
  GCTTTGTGCAGCTGGAAGGCCTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGACGGGA
  AGGTCGAGTGGCCAATGTGGCTGATGTTGTGAGTAAAGGCCAGAGAGTCAAGGTCAAAGTTCTGTCCTTC
  ACTGGAACCAAGACCAGCCTGAGCATGAAGGATGTGGACCAAGAGACAGGGGAAGACCTAAACCCAAACA
  GACGGAGAAATCTGGTTGGCGAGACCAATGAAGAGACGTCAATGCGGAACCCAGACAGACCCACTCACCT
  CTCCCTTGTCAGCGCTCCTGAAGTAGAGGATGACTCGCTGGAGCGCAAGCGGCTCACCCGAATCTCTGAC
  CCAGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTGCTTTCCAAAGAAGAGTTTCCAGACTTCG
  ACGAAGAGACTGGCATTCTCCCCAAGGTGGATGATGAAGAAGATGAGGATCTTGAAATTGAACTGGTTGA
  AGAGGAGCCTCCGTTCCTGAGAGGGCATACCAAGCAGAGCATGGATATGAGCCCCATCAAAATCGTTAAG
  AACCCAGATGGCTCACTCTCCCAAGCAGCCATGATGCAGAGTGCCCTGGCCAAGGAAAGACGGGAGCTGA
  AGCAGGCCCAGCGGGAAGCAGAGATGGATTCTATACCCATGGGGCTCAACAAACACTGGGTTGATCCCTT
  GCCCGATGCGGAAGGCAGGCAGATTGCTGCCAACATGAGGGGGATTGGGATGATGCCCAACGACATTCCT
  GAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCTTCTTATGGGAAGAAGACCCAGATGTCAATCCTTG
  AGCAGAGAGAGAGCCTGCCCATCTACAAACTGAAGGAGCAGCTGGTGCAGGCTGTGCATGACAATCAGAT
  CCTAATTGTAATTGGAGAGACAGGGTCTGGGAAGACAACACAGATCACCCAGTACCTGGCAGAGGCAGGC
  TATACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCTGTGGCCAAAAGAG
  TCTCAGAAGAGTTTGGTTGTTGCCTTGGTCAAGAGGTGGGCTACACCATTCGATTTGAGGATTGCACCAG
  CCCAGAAACAGTCATCAAGTACATGACAGATGGCATGCTGCTAAGAGAGTGCCTGATTGACCCCGATCTC
  ACTCAGTATGCCATCATTATGTTGGATGAAGCACACGAGAGGACCATTCACACAGATGTGCTCTTTGGAT
  TGTTGAAAAAGACAGTTCAAAAACGACAAGACATGAAGCTGATTGTCACCTCAGCCACGCTGGATGCGGT
  GAAGTTTTCTCAGTACTTCTATGAAGCACCCATCTTCACAATCCCAGGTCGAACCTATCCAGTGGAAATT
  CTGTACACAAAGGAGCCGGAAACAGACTATTTGGACGCTAGCCTGATCACGGTCATGCAGATCCATCTGA
  CAGAGCCGCCAGGTGACATCTTGGTTTTCTTGACTGGTCAGGAAGAGATCGACACTGCTTGTGAGATCCT
  GTATGAAAGAATGAAGTCCCTGGGACCTGATGTCCCAGAGTTGATCATCCTCCCAGTGTACTCTGCCCTT
  CCGAGTGAGATGCAGACCCGAATCTTTGATCCAGCTCCCCCTGGTAGCAGAAAGGTGGTGATTGCCACCA
  ACATTGCAGAAACATCTCTGACCATCGACGGCATCTACTATGTGGTTGACCCTGGATTTGTGAAGCAGAA
  AGTGTACAATTCTAAAACAGGGATCGATCAGCTTGTGGTGACACCTATTTCTCAGGCTCAAGCAAAGCAG
  AGAGCTGGCCGGGCTGGGAGAACTGGCCCAGGGAAGTGTTACAGACTGTACACGGAGCGTGCCTACCGCG
  ATGAAATGCTGACCACCAATGTACCGGAAATCCAGAGAACCAACCTGGCCAGCACAGTGCTGTCCCTCAA
  GGCCATGGGTATTAATGACCTGCTGTCCTTTGACTTCATGGATGCCCCACCGATGGAAACTCTGATCACG
  GCCATGGAGCAGCTGTATACACTGGGGGCCCTGGACGACGAGGGCCTGCTCACTCGCCTGGGCCGCAGGA
  TGGCAGAGTTCCCTCTGGAGCCAATGCTGTGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGCGA
  AGAGATGCTAACTATCGTGTCCATGCTGTCTGTGCAGAACGTCTTCTACCGACCCAAGGATAAACAGGCC
  CTTGCAGATCAGAAGAAAGCCAAATTCCACCAGACAGAAGGGGACCACCTCACCCTGCTGGCCGTATACA
  ACTCCTGGAAGAACAACAAATTCTCCAACCCGTGGTGCTATGAGAATTTTATTCAGGCTCGTTCCCTACG
  CCGTGCCCAGGACATTCGGAAGCAGATGTTAGGCATAATGGACAGGCACAAGCTGGACGTGGTCTCCTGT
  GGCAAATCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGGAACGCTGCCAAGAAGGACC
  CACAGGAAGGCTACCGGACTCTGATAGACCAGCAGGTGGTGTATATCCACCCCTCCAGTGCTCTCTTCAA
  CAGACAGCCCGAATGGGTGGTATACCACGAACTTGTGCTGACCACAAAGGAATACATGCGTGAGGTCACC
  ACCATCGACCCCCGGTGGCTTGTGGAGTTTGCTCCGGCTTTCTTCAAAGTTTCTGACCCAACCAAGCTAA
  GCAAGCAGAAGAAACAGCAGCGCCTGGAGCCTCTGTACAATCGGTATGAGGAGCCGAACGCCTGGAGAAT
  CTCCCGAGCCTTCCGCCGGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226459 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVAVAAAGVLMGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL
  VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLRELFPVLCQPDNPSARTMLDEEDVK
  VAVDVLKELEALMPSAAGQEKQRDPEHRDRTKKKKRSRSRDRDRDRDRDRDRDRDRDRDRDKDRERDRDR
  ERDRERDRERDHKRRHRSRSRSHSRTRERTKGKSRYRSRSRSQSPFKDRKDREKYGERNLDRWRDKHVDR
  PPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSF
  TGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISD
  PEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVK
  NPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIP
  EWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAG
  YTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDL
  TQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEI
  LYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSAL
  PSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQ
  RAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLIT
  AMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQA
  LADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSC
  GKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVT
  TIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144831
• ORF Size: 3735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144831.2, NP_659080.2
• RefSeq Size: 4344 bp
• RefSeq ORF: 3735 bp
• Locus ID: 217207
• UniProt ID: A2A4P0
• Cytogenetics: 11 D
• MW: 142.6 kDa
• Gene Summary: Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.[UniProtKB/Swiss-Prot Function]