Dhx8 (NM_144831) Mouse Tagged ORF Clone
CAT#: MG226459
- TrueORF®
Dhx8 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_144831" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Dhx8 |
Synonyms | Ddx8; mDEAH6; mKIAA4096 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226459 representing NM_144831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTAGCCGTGGCCGCCGCCGGGGTCCTAATGGGCTCGGAGCCCGGCCCGGCGGAAGAACTCGCTA AACTTGAGTACCTGTCTTTGGTGTCGAAGGTTTGCACGGAGCTGGACAATCACTTGGGCATCAACGACAA GGATCTGGCTGAATTTGTCATCAGTCTTGCTGAGAAAAATACTACCTTTGATACTTTTAAGGCTTCTCTG GTCAAAAATGGTGCAGAATTCACAGATTCTCTTATTAGCAACCTCCTGCGTCTCATACAGACCATGCGGC CTCCTGCAAAGCCTTCTACCAGTAAAGATCCGGTTGTTAAACCCAAAACCGAAAAAGAAAAGCTGAGGGA ACTCTTCCCCGTCCTCTGCCAGCCGGACAACCCTTCAGCTCGGACCATGTTGGATGAGGAAGATGTGAAA GTCGCTGTGGACGTCTTGAAAGAGCTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAAGAGAAACAAAGAG ACCCTGAGCACAGGGACAGGACAAAGAAGAAGAAGAGGAGTCGGAGCCGAGACCGAGATCGAGACCGAGA CCGAGACCGGGACCGGGACCGGGACCGGGACAGGGACCGTGACAAGGACCGAGAACGAGATAGAGATCGA GAACGAGACAGAGAGCGAGATAGAGAGAGAGACCATAAGCGGAGACACCGATCACGCTCTCGGTCACATT CTAGAACCCGGGAGAGGACTAAGGGGAAATCTAGATACAGATCTAGGAGCAGAAGTCAGAGTCCCTTCAA AGATCGGAAAGACCGAGAAAAGTATGGGGAGAGGAACTTGGACAGATGGCGGGATAAGCATGTGGACCGC CCTCCCCCGGAAGAGCCTGCCATTGGAGATATTTACAATGGCAAAGTGACCAGTATCATGCAGTTCGGTT GCTTTGTGCAGCTGGAAGGCCTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGACGGGA AGGTCGAGTGGCCAATGTGGCTGATGTTGTGAGTAAAGGCCAGAGAGTCAAGGTCAAAGTTCTGTCCTTC ACTGGAACCAAGACCAGCCTGAGCATGAAGGATGTGGACCAAGAGACAGGGGAAGACCTAAACCCAAACA GACGGAGAAATCTGGTTGGCGAGACCAATGAAGAGACGTCAATGCGGAACCCAGACAGACCCACTCACCT CTCCCTTGTCAGCGCTCCTGAAGTAGAGGATGACTCGCTGGAGCGCAAGCGGCTCACCCGAATCTCTGAC CCAGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTGCTTTCCAAAGAAGAGTTTCCAGACTTCG ACGAAGAGACTGGCATTCTCCCCAAGGTGGATGATGAAGAAGATGAGGATCTTGAAATTGAACTGGTTGA AGAGGAGCCTCCGTTCCTGAGAGGGCATACCAAGCAGAGCATGGATATGAGCCCCATCAAAATCGTTAAG AACCCAGATGGCTCACTCTCCCAAGCAGCCATGATGCAGAGTGCCCTGGCCAAGGAAAGACGGGAGCTGA AGCAGGCCCAGCGGGAAGCAGAGATGGATTCTATACCCATGGGGCTCAACAAACACTGGGTTGATCCCTT GCCCGATGCGGAAGGCAGGCAGATTGCTGCCAACATGAGGGGGATTGGGATGATGCCCAACGACATTCCT GAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCTTCTTATGGGAAGAAGACCCAGATGTCAATCCTTG AGCAGAGAGAGAGCCTGCCCATCTACAAACTGAAGGAGCAGCTGGTGCAGGCTGTGCATGACAATCAGAT CCTAATTGTAATTGGAGAGACAGGGTCTGGGAAGACAACACAGATCACCCAGTACCTGGCAGAGGCAGGC TATACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCTGTGGCCAAAAGAG TCTCAGAAGAGTTTGGTTGTTGCCTTGGTCAAGAGGTGGGCTACACCATTCGATTTGAGGATTGCACCAG CCCAGAAACAGTCATCAAGTACATGACAGATGGCATGCTGCTAAGAGAGTGCCTGATTGACCCCGATCTC ACTCAGTATGCCATCATTATGTTGGATGAAGCACACGAGAGGACCATTCACACAGATGTGCTCTTTGGAT TGTTGAAAAAGACAGTTCAAAAACGACAAGACATGAAGCTGATTGTCACCTCAGCCACGCTGGATGCGGT GAAGTTTTCTCAGTACTTCTATGAAGCACCCATCTTCACAATCCCAGGTCGAACCTATCCAGTGGAAATT CTGTACACAAAGGAGCCGGAAACAGACTATTTGGACGCTAGCCTGATCACGGTCATGCAGATCCATCTGA CAGAGCCGCCAGGTGACATCTTGGTTTTCTTGACTGGTCAGGAAGAGATCGACACTGCTTGTGAGATCCT GTATGAAAGAATGAAGTCCCTGGGACCTGATGTCCCAGAGTTGATCATCCTCCCAGTGTACTCTGCCCTT CCGAGTGAGATGCAGACCCGAATCTTTGATCCAGCTCCCCCTGGTAGCAGAAAGGTGGTGATTGCCACCA ACATTGCAGAAACATCTCTGACCATCGACGGCATCTACTATGTGGTTGACCCTGGATTTGTGAAGCAGAA AGTGTACAATTCTAAAACAGGGATCGATCAGCTTGTGGTGACACCTATTTCTCAGGCTCAAGCAAAGCAG AGAGCTGGCCGGGCTGGGAGAACTGGCCCAGGGAAGTGTTACAGACTGTACACGGAGCGTGCCTACCGCG ATGAAATGCTGACCACCAATGTACCGGAAATCCAGAGAACCAACCTGGCCAGCACAGTGCTGTCCCTCAA GGCCATGGGTATTAATGACCTGCTGTCCTTTGACTTCATGGATGCCCCACCGATGGAAACTCTGATCACG GCCATGGAGCAGCTGTATACACTGGGGGCCCTGGACGACGAGGGCCTGCTCACTCGCCTGGGCCGCAGGA TGGCAGAGTTCCCTCTGGAGCCAATGCTGTGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGCGA AGAGATGCTAACTATCGTGTCCATGCTGTCTGTGCAGAACGTCTTCTACCGACCCAAGGATAAACAGGCC CTTGCAGATCAGAAGAAAGCCAAATTCCACCAGACAGAAGGGGACCACCTCACCCTGCTGGCCGTATACA ACTCCTGGAAGAACAACAAATTCTCCAACCCGTGGTGCTATGAGAATTTTATTCAGGCTCGTTCCCTACG CCGTGCCCAGGACATTCGGAAGCAGATGTTAGGCATAATGGACAGGCACAAGCTGGACGTGGTCTCCTGT GGCAAATCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGGAACGCTGCCAAGAAGGACC CACAGGAAGGCTACCGGACTCTGATAGACCAGCAGGTGGTGTATATCCACCCCTCCAGTGCTCTCTTCAA CAGACAGCCCGAATGGGTGGTATACCACGAACTTGTGCTGACCACAAAGGAATACATGCGTGAGGTCACC ACCATCGACCCCCGGTGGCTTGTGGAGTTTGCTCCGGCTTTCTTCAAAGTTTCTGACCCAACCAAGCTAA GCAAGCAGAAGAAACAGCAGCGCCTGGAGCCTCTGTACAATCGGTATGAGGAGCCGAACGCCTGGAGAAT CTCCCGAGCCTTCCGCCGGCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226459 representing NM_144831
Red=Cloning site Green=Tags(s) MAVAVAAAGVLMGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLRELFPVLCQPDNPSARTMLDEEDVK VAVDVLKELEALMPSAAGQEKQRDPEHRDRTKKKKRSRSRDRDRDRDRDRDRDRDRDRDRDKDRERDRDR ERDRERDRERDHKRRHRSRSRSHSRTRERTKGKSRYRSRSRSQSPFKDRKDREKYGERNLDRWRDKHVDR PPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSF TGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISD PEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVK NPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIP EWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAG YTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDL TQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEI LYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSAL PSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQ RAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLIT AMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQA LADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSC GKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVT TIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144831 |
ORF Size | 3732 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144831.2, NP_659080.2 |
RefSeq Size | 4344 bp |
RefSeq ORF | 3735 bp |
Locus ID | 217207 |
UniProt ID | A2A4P0 |
Cytogenetics | 11 D |
Gene Summary | Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224038 | Dhx8 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), (10ug) |
USD 1,192.00 |
|
MR226459 | Dhx8 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8) |
USD 1,084.00 |
|
MR226459L3 | Lenti ORF clone of Dhx8 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8) |
USD 1,384.00 |
|
MR226459L4 | Lenti ORF clone of Dhx8 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8) |
USD 1,384.00 |
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