Pik3r1 (NM_001077495) Mouse Tagged Lenti ORF Clone
CAT#: MR226181L4
- LentiORF®
Lenti ORF clone of Pik3r1 (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2
"NM_001077495" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Pik3r1 |
Synonyms | p50alpha; p55alpha; p85alpha; PI3K |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR226181).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001077495 |
ORF Size | 2172 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001077495.2, NP_001070963.1 |
RefSeq Size | 6928 bp |
RefSeq ORF | 2175 bp |
Locus ID | 18708 |
UniProt ID | P26450 |
Cytogenetics | 13 53.92 cM |
Gene Summary | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221084 | Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2, (10ug) |
USD 730.00 |
|
MG226181 | Pik3r1 (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) (Pik3r1) transcript variant 2, (10ug) |
USD 863.00 |
|
MR226181 | Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2 |
USD 663.00 |
|
MR226181L3 | Lenti ORF clone of Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2 |
USD 963.00 |
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