Pik3r1 (NM_001077495) Mouse Tagged ORF Clone
CAT#: MR226181
- TrueORF®
Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001077495" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pik3r1 |
Synonyms | p50alpha; p55alpha; p85alpha; PI3K |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226181 representing NM_001077495
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCAGAGGGCTACCAGTACAGAGCACTGTACGACTACAAGAAGGAGCGAGAGGAAGACATTGACC TACACCTGGGGGACATACTGACTGTGAATAAAGGCTCCTTAGTGGCACTTGGATTCAGTGATGGCCAGGA AGCCCGGCCTGAAGATATTGGCTGGTTAAATGGCTACAATGAAACCACTGGGGAGAGGGGAGACTTTCCA GGAACTTACGTTGAATACATTGGAAGGAAAAGAATTTCACCCCCTACTCCCAAGCCTCGGCCCCCTCGAC CGCTTCCTGTTGCTCCGGGTTCTTCAAAAACTGAAGCTGACACGGAGCAGCAAGCGTTGCCCCTTCCTGA CCTGGCCGAGCAGTTTGCCCCTCCTGATGTTGCCCCGCCTCTCCTTATAAAGCTCCTGGAAGCCATTGAG AAGAAAGGACTGGAATGTTCGACTCTATACAGAACACAAAGCTCCAGCAACCCTGCAGAATTACGACAGC TTCTTGATTGTGATGCCGCGTCAGTGGACTTGGAGATGATCGACGTACACGTCTTAGCAGATGCTTTCAA ACGCTATCTCGCCGACTTACCAAATCCTGTCATTCCTGTAGCTGTTTACAATGAGATGATGTCTTTAGCC CAAGAACTACAGAGCCCTGAAGACTGCATCCAGCTGTTGAAGAAGCTCATTAGATTGCCTAATATACCTC ATCAGTGTTGGCTTACGCTTCAGTATTTGCTCAAGCATTTTTTCAAGCTCTCTCAAGCCTCCAGCAAAAA CCTTTTGAATGCAAGAGTCCTCTCTGAGATTTTCAGCCCCGTGCTTTTCAGATTTCCAGCCGCCAGCTCT GATAATACTGAACACCTCATAAAAGCGATAGAGATTTTAATCTCAACGGAATGGAATGAGAGACAGCCAG CACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGTAGCCAACAACAGCATGAACAACAATATGTC CTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAACTCCGAGACACT GCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAATGCACGGCGATTACACTCTTACACTAAGGA AAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTAAC CTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTTAGCTCAGTACAACCCCAAGCTG GATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGGATCAAGTTGTCAAAGAAGATAATATTGAAG CTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGATAGATTATA TGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAAAGAACGGCTATCGAAGCATTTAATGAAACC ATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAAAC GCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATAAGCTGAAGTCGCGTATCAGTGA GATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTACCGAGAGATCGAC AAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAATACTTGATGTGGC TGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTGGCTGGGGAATGAAAATACCGAAGATCAATA CTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCAAC CGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTCCTTGTCCGGGAGAGCAGTAAGC AGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGTCAAGCATTGCGTCATTAACAAGACTGCCAC CGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTACATTATCAACAC ACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACACTAGCATACCCAGTATATGCACAACAGAGGC GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226181 representing NM_001077495
Red=Cloning site Green=Tags(s) MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEDIGWLNGYNETTGERGDFP GTYVEYIGRKRISPPTPKPRPPRPLPVAPGSSKTEADTEQQALPLPDLAEQFAPPDVAPPLLIKLLEAIE KKGLECSTLYRTQSSSNPAELRQLLDCDAASVDLEMIDVHVLADAFKRYLADLPNPVIPVAVYNEMMSLA QELQSPEDCIQLLKKLIRLPNIPHQCWLTLQYLLKHFFKLSQASSKNLLNARVLSEIFSPVLFRFPAASS DNTEHLIKAIEILISTEWNERQPAPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDT ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKL DVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNET IKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREID KRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSN RNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQH TSLVQHNDSLNVTLAYPVYAQQRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001077495 |
ORF Size | 2172 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001077495.2, NP_001070963.1 |
RefSeq Size | 6928 bp |
RefSeq ORF | 2175 bp |
Locus ID | 18708 |
UniProt ID | P26450 |
Cytogenetics | 13 53.92 cM |
MW | 83.5 kDa |
Gene Summary | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221084 | Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2, (10ug) |
USD 730.00 |
|
MG226181 | Pik3r1 (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) (Pik3r1) transcript variant 2, (10ug) |
USD 863.00 |
|
MR226181L3 | Lenti ORF clone of Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2 |
USD 963.00 |
|
MR226181L4 | Lenti ORF clone of Pik3r1 (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2 |
USD 963.00 |
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