Dnmt3a (NM_153743) Mouse Tagged ORF Clone
CAT#: MR226152
- TrueORF®
DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_153743" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dnmt3a |
Synonyms | MmuIIIA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226152 representing NM_153743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCTGTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTG TGCAGCAGCCCACGGACCCTGCTTCTCCGACTGTGGCCACCACCCCTGAGCCAGTAGGAGGGGATGCTGG GGACAAGAATGCTACCAAAGCAGCCGACGATGAGCCTGAGTATGAGGATGGCCGGGGCTTTGGCATTGGA GAGCTGGTGTGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAG GCCGGAGCCGAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAGTTCTCAGTGGTGTG TGTGGAGAAGCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTCCACCAGGCCACCTACAACAAGCAGCCC ATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCCGGGAAGCTGTTTCCAGCTT GCCATGACAGTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGTGCAGAACAAGCAGATGATTGAATGGGC CCTCGGTGGCTTCCAGCCCTCGGGTCCTAAGGGCCTGGAGCCACCAGAAGAAGAGAAGAATCCTTACAAG GAAGTTTACACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTTACGCCCCACCCCCACCAGCCAAGAAAC CCAGAAAGAGCACAACAGAGAAACCTAAGGTCAAGGAGATCATTGATGAGCGCACAAGGGAGCGGCTGGT GTATGAGGTGCGCCAGAAGTGCAGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTCACC CTGGAGCACCCACTCTTCATTGGAGGCATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACC AGTATGACGACGATGGGTACCAGTCCTATTGCACCATCTGCTGTGGGGGGCGTGAAGTGCTCATGTGTGG GAACAACAACTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGATCTCTTGGTGGGGCCAGGAGCTGCTCAG GCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCATAAGGGCACCTATGGGCTGCTGCGAA GACGGGAAGACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAATAACCATGACCAGGAATTTGACCCCCC AAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGCGTGCTGTCTCTCTTTGATGGGATT GCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATTGCCTCCGAGGTGTGTG AGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGT CACACAGAAGCATATCCAGGAGTGGGGCCCATTCGACCTGGTGATTGGAGGCAGTCCCTGCAATGACCTC TCCATTGTCAACCCTGCCCGCAAGGGACTTTATGAGGGTACTGGCCGCCTCTTCTTTGAGTTCTACCGCC TCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGC CATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTTGAGTCTAACCCCGTGATGATTGACGCCAAA GAAGTGTCTGCTGCACACAGGGCCCGTTACTTCTGGGGTAACCTTCCTGGCATGAACAGGCCTTTGGCAT CCACTGTGAATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCACGGCAGAATAGCCAAGTTCAGCAAAGT GAGGACCATTACCACCAGGTCAAACTCTATAAAGCAGGGCAAAGACCAGCATTTCCCCGTCTTCATGAAC GAGAAGGAGGACATCCTGTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCCGTCCACTACACAGACG TCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCGGTCATCCGCCA CCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226152 representing NM_153743
Red=Cloning site Green=Tags(s) MNAVEENQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGGDAGDKNATKAADDEPEYEDGRGFGIG ELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQP MYRKAIYEVLQVASSRAGKLFPACHDSDESDSGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYK EVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVT LEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQ AAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGI ATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDL SIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAK EVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMN EKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153743 |
ORF Size | 2067 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153743.4, NP_714965.1 |
RefSeq Size | 8913 bp |
RefSeq ORF | 2070 bp |
Locus ID | 13435 |
UniProt ID | O88508 |
Cytogenetics | 12 A1.1 |
MW | 78.3 kDa |
Gene Summary | This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226152 | DNMT3A (tGFP-tagged) - Mouse DNA methyltransferase 3A (DNMT3A) transcript variant 2 |
USD 894.00 |
|
MR226152L3 | Lenti ORF clone of DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 2 |
USD 994.00 |
|
MR226152L4 | Lenti ORF clone of DNMT3A (mGFP-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 2 |
USD 994.00 |
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