Kcnj5 (NM_010605) Mouse Tagged ORF Clone

CAT#: MR226110

Kcnj5 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 5 (Kcnj5)

Product Images

Circular map for MR226110

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj5
• Synonyms: GIRK4; Kir3.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226110 representing NM_010605
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTGATTCTAGGAATGCTATGAATCAAGACATGGAGATAGGAGTCACTTCCCAGGACCACAAGA
  AGATTCCCAAACAGGCTCGGGATTACATCCCCATTGCCACAGACCGCACCCGCCTGCTGACAGAAGGCAA
  GAAGCCACGCCAGCGCTACATGGAGAAGACTGGCAAGTGCAATGTACACCACGGCAATGTTCAGGAAACC
  TACCGCTACCTAAGTGACCTCTTCACCACCCTGGTGGACCTCAAATGGCGCTTCAACCTTCTGGTCTTCA
  CCATGGTCTACACCATCACCTGGCTGTTCTTTGGCTTCATTTGGTGGCTCATTGCTTATGTCCGAGGTGA
  TCTGGATCACGTGGGTGACCAAGAGTGGATCCCTTGTGTTGAAAACCTTAGCGGCTTTGTATCTGCTTTC
  CTGTTCTCCATCGAGACAGAAACAACCATTGGGTATGGCTTCAGAGTCATTACAGAGAAGTGTCCAGAAG
  GGATCATACTCCTTCTGGTGCAGGCCATTCTGGGCTCGATTGTTAATGCCTTCATGGTGGGGTGCATGTT
  TGTAAAGATCAGCCAGCCAAAGAAGAGAGCAGAGACCCTAATGTTTTCCAACAATGCCGTCATCTCCATG
  CGGGATGAGAAGCTGTGCCTCATGTTCCGGGTAGGGGACCTCCGAAACTCCCACATCGTGGAGGCCTCCA
  TCCGTGCCAAGCTTATCAAGTCCCGGCAGACCAAAGAAGGAGAATTCATCCCCTTGAACCAGACCGACAT
  TAATGTGGGCTTTGACACTGGTGACGACCGCCTCTTCCTGGTCTCCCCACTCATCATCTCCCACGAGATC
  AACGAGAAGAGCCCTTTTTGGGAGATGTCTCGTGCTCAACTGGAACAGGAAGAGTTCGAAGTTGTGGTCA
  TACTAGAAGGAATGGTAGAAGCAACAGGCATGACTTGCCAAGCACGGAGCTCTTACATGGATACAGAGGT
  GCTCTGGGGTCACCGATTCACACCAGTTCTCACCTTGGAAAAGGGCTTCTATGAGGTGGACTACAACACT
  TTCCATGACACCTATGAGACCAACACACCCAGTTGCTGTGCCAAGGAGCTGGCGGAGATGAAGCGGAGCG
  GTCGGCTCCTCCAGTACCTTCCCAGTCCTCCCTTGCTTGGGGGCTGTGCTGAGGCTGGGAACGAAGCTGA
  GGCCGAGAAGGATGAAGAGGGTGAGCCCAATGGACTGAGTGTGTCCCAGGCCACCAGGGGCTCAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226110 representing NM_010605
  Red=Cloning site Green=Tags(s)
  
  MAGDSRNAMNQDMEIGVTSQDHKKIPKQARDYIPIATDRTRLLTEGKKPRQRYMEKTGKCNVHHGNVQET
  YRYLSDLFTTLVDLKWRFNLLVFTMVYTITWLFFGFIWWLIAYVRGDLDHVGDQEWIPCVENLSGFVSAF
  LFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISM
  RDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEI
  NEKSPFWEMSRAQLEQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNT
  FHDTYETNTPSCCAKELAEMKRSGRLLQYLPSPPLLGGCAEAGNEAEAEKDEEGEPNGLSVSQATRGSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010605
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010605.5
• RefSeq Size: 4496 bp
• RefSeq ORF: 1260 bp
• Locus ID: 16521
• UniProt ID: P48545
• Cytogenetics: 9 17.65 cM
• MW: 48.1 kDa
• Gene Summary: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.[UniProtKB/Swiss-Prot Function]