Ing1 (NM_011919) Mouse Tagged ORF Clone
CAT#: MR225175
- TrueORF®
Ing1 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 1 (Ing1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011919" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ing1 |
Synonyms | 2610028J21Rik; AA407184; AI875420; mING1h; p33Ing1; p37Ing1b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225175 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAGTCCTGCCAACGGGGAGCAGATCCACCTGGTGAACTATGTGGAGGATTACCTGGACTCAATCG AGTCACTGCCTTTCGACCTGCAGAGGAACGTCTCGCTGATGCGGGAGATCGACGCCAAATACCAAGAGAT CCTGAAGGAGCTGGACGACTACTATGAGAAGTTCAAACGGGAGACAGACGGCACCCAGAAGCGCCGGGTA CTGCACTGCATCCAGAGGGCCCTGATCCGCAGCCAGGAGCTAGGCGATGAGAAGATCCAGATCGTGAGTC AGATGGTGGAGCTGGTGGAGAACCGCAGCAGACAGGTGGACAGTCACGTGGAGCTCTTCGAAGCACACCA GGACATCAGTGACGGCACTGGTGGCAGCGGCAAGGCGGGCCAGGACAAGTCGAAGAGTGAGGCCATCACA CAGGCAGATAAGCCGAATAACAAGCGGTCCAGGAGGCAGCGAAACAATGAGAATCGAGAGAACGCGTCGA ATAATCACGACCATGATGACATCACCTCAGGAACGCCCAAGGAGAAGAAAGCAAAAACCTCAAAGAAGAA GAAACGCTCCAAGGCCAAAGCAGAGAGGGAAGCGTCTCCTGCCGACCTTCCCATCGACCCCAACGAGCCC ACGTACTGTCTGTGCAACCAGGTCTCCTACGGGGAGATGATCGGCTGTGACAACGACGAATGCCCCATCG AGTGGTTCCACTTCTCCTGCGTGGGGCTCAACCATAAACCAAAGGGCAAGTGGTACTGCCCCAAGTGCCG TGGGGAGAGCGAGAAGACCATGGACAAAGCCCTGGAGAAGTCCAAGAAAGAGAGGGCTTACAACAGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR225175 protein sequence
Red=Cloning site Green=Tags(s) MLSPANGEQIHLVNYVEDYLDSIESLPFDLQRNVSLMREIDAKYQEILKELDDYYEKFKRETDGTQKRRV LHCIQRALIRSQELGDEKIQIVSQMVELVENRSRQVDSHVELFEAHQDISDGTGGSGKAGQDKSKSEAIT QADKPNNKRSRRQRNNENRENASNNHDHDDITSGTPKEKKAKTSKKKKRSKAKAEREASPADLPIDPNEP TYCLCNQVSYGEMIGCDNDECPIEWFHFSCVGLNHKPKGKWYCPKCRGESEKTMDKALEKSKKERAYNR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011919 |
ORF Size | 840 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011919.5, NP_036049.2 |
RefSeq Size | 2813 bp |
RefSeq ORF | 840 bp |
Locus ID | 26356 |
UniProt ID | Q9QXV3 |
Cytogenetics | 8 A1.1 |
MW | 32.1 kDa |
Gene Summary | Isoform 1 inhibits p53-dependent transcriptional activation and may function as an oncoprotein. Isoform 2 acts as a negative growth regulator by cooperating with p53 in transcriptional activation of p53-responsive genes and may act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209707 | Ing1 (untagged) - Mouse inhibitor of growth family, member 1 (Ing1), (10ug) |
USD 330.00 |
|
MG225175 | Ing1 (tGFP-tagged) - Mouse inhibitor of growth family member 1 (Ing1), (10ug) |
USD 500.00 |
|
MR225175L3 | Lenti ORF clone of Ing1 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 1 (Ing1) |
USD 600.00 |
|
MR225175L4 | Lenti ORF clone of Ing1 (mGFP-tagged) - Mouse inhibitor of growth family, member 1 (Ing1) |
USD 600.00 |
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