Cadps (NM_001042617) Mouse Tagged ORF Clone

CAT#: MR224888

Cadps (Myc-DDK-tagged) - Mouse Ca2+-dependent secretion activator (Cadps), transcript variant 2

Product Images

Circular map for MR224888

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cadps
• Synonyms: AU067781; CAPS; CAPS1; mKIAA1121
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224888 representing NM_001042617
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACCCTTCGTCCAGCGAAGAGGAGTCAGATGAGATCCTGGAAGAGGAGAGGGGCAAGGATGTGC
  TGGGCTCGGCCGCGTCTGGAGCGCGCCTGTCTCCCAGCCGCACCAGCGAGGGCTCGGCAGGCAGCGCCGG
  GATGGGGGGCAGTGGCGCCGGGGCCGGGGTGGGCGCCGGCGGCGGTGGGGGTAGCGGCGCGAGCAGCGGC
  GGCGGGGCCGGGGGGCTGCAGCCCAGCAGCCGCGCCGGCGGCGGCCGGCCTTCCAGCCCTAGCCCGTCGG
  TGGTGAGCGAGAAGGAGAAGGAAGAGTTGGAGAGGCTGCAGAAGGAGGAGGAAGAGAGGAAGAAGAGGCT
  GCAGCTGTACGTGTTCGTGATGCGCTGCATCGCCTACCCCTTCAACGCCAAGCAGCCCACCGACATGGCT
  CGGCGGCAGCAGAAGATCAGCAAACAGCAGCTACAGACTGTCAAAGACCGGTTCCAGGCTTTTCTCAATG
  GGGAGACCCAAATCGTAGCTGATGAAGCCTTTATGAATGCTGTCCAGAGCTACTATGAGGTGTTTCTCAA
  GAGTGACCGTGTGGCCCGCATGGTGCAGAGTGGGGGCTGCTCAGCCAATGACTCTCGGGAGGTCTTCAAG
  AAACACATCGAGAAGAGGGTGCGCAGCCTACCTGAGATTGACGGCCTCAGCAAGGAGACAGTGCTAAGCT
  CTTGGATGGCCAAGTTTGATGCCATCTACCGTGGTGAGGAGGACCCCAGGAAGCAGCAAGCCCGGATGAC
  AGCCAGTGCAGCTTCTGAGTTGATTCTGAGCAAGGAACAGCTCTATGAGATGTTTCAGAACATTCTTGGG
  ATCAAGAAGTTTGAACATCAACTCCTGTATAATGCCTGTCAGCTGGACAATCCAGATGAGCAGGCAGCCC
  AGATCAGACGAGAGCTGGATGGACGTCTCCAAATGGCAGATCAAATAGCCAGGGAACGCAAATTTCCTAA
  GTTCGTATCCAAAGAAATGGAAAACATGTACATTGAGGAGCTGAAGTCCTCCGTCAACCTGCTCATGGCC
  AACTTGGAGAGCATGCCGGTGTCCAAGGGTGGAGAGTTCAAGCTACAGAAACTGAAACGCAGTCACAATG
  CTTCCATCATTGACATGGGTGAGGAGAGTGAGAACCAGCTTTCTAAGTCAGATGTCCTGCTGTCCTTCTC
  CTTGGAGGTGGTGATCATGGAAGTCCAAGGCCTTAAGTCCTTGGCTCCAAATCGAATTGTATACTGCACA
  ATGGAGGTGGAGGGTGGAGAGAAACTTCAGACAGATCAAGCAGAGGCTTCTAAACCAACCTGGGGCACTC
  AAGGCGACTTCTCCACAACCCATGCGCTGCCAGCTGTTAAAGTAAAACTATTCACAGAGAGCACTGGTGT
  CCTGGCCTTGGAAGACAAGGAACTCGGGCGGGTGATCCTCCATCCCACCCCAAATAGCCCCAAACAATCT
  GAGTGGCACAAAATGACAGTCTCCAAAAACTGCCCCGATCAAGACCTCAAAATCAAACTTGCTGTCCGAA
  TGGATAAGCCTCAAAACATGAAGCACTCTGGGTACTTATGGACCATTGGTAAGAATGTGTGGAAGAGGTG
  GAAGAAGAGGTTCTTTGTGTTGGTGCAGGTCAGCCAGTACACTTTTGCCATGTGCAGCTATCGAGAAAAG
  AAGGCAGAGCCTCAGGAACTCCTACAGCTGGACGGCTACACCGTGGATTACACCGACCCTCAGCCAGGTT
  TGGAGGGTGGCCGAGCCTTCTTCAATGCAGTCAAAGAAGGAGACACGGTGATATTTGCAAGTGATGATGA
  ACAAGATCGAATTTTGTGGGTCCAAGCTATGTACAGGGCCACTGGCCAGTCGCACAAACCTGTTCCCCCT
  ACCCAGGTGCAGAAGCTCAACGCCAAGGGCGGAAATGTGCCTCAGCTGGATGCCCCCATCTCACAATTTT
  CTGGACTGAAGGACGCAGATAGAGCTCAGAAGCATGGCATGGATGAATTTATCTCTTCCAACCCCTGTAA
  CTTTGACCACGCTTCCCTCTTTGAGATGGTGCAGCGGCTGACTTTGGATCACAGACTTAATGATTCCTAT
  TCTTGCCTGGGCTGGTTCAGTCCTGGCCAGGTGTTTGTGTTAGATGAGTATTGCGCCAGAAATGGAGTCC
  GAGGATGTCACCGGCATCTCTGCTACCTTAGAGACTTGCTGGAACGAGCAGAAAATGGCGCGATGATTGA
  CCCCACGCTTCTTCACTACAGCTTTGCCTTCTGCGCGTCCCATGTCCATGGTAACAGGCCTGATGGAATC
  GGAACTGTGACTGTTGAAGAAAAAGAACGCTTTGAAGAAATCAAAGAGAGACTCCGAGTTCTGCTGGAAA
  ATCAAATTACACATTTCAGGTACTGCTTTCCATTTGGTCGACCTGAAGGTGCTTTGAAAGCTACTCTCTC
  ACTCTTGGAACGGGTTTTGATGAAAGATATTGTCACTCCAGTACCACAAGAAGAGGTCAAAACTGTCATC
  CGTAAATGCCTAGAGCAGGCTGCTCTAGTCAACTACTCACGCCTATCTGAGTATGCCAAAATTGAAGAGA
  ATCAAAAGGATGCAGAAAATGTAGGCCGCTTAATCACTCCCGCCAAAAAGCTCGAAGACACAATCCGTCT
  TGCTGAACTAGTCATCGAAGTACTGCAACAAAACGAGGAGCACCACGCCGAGCCACATGTTGATAAAGGA
  GAAGCCTTTGCGTGGTGGTCAGACTTAATGGTGGAGCATGCGGAGACGTTCCTGTCACTCTTTGCAGTGG
  ACATGGATGCAGCCTTAGAGGTGCAACCCCCAGACACGTGGGACAGTTTCCCACTCTTCCAGCTGCTGAA
  TGACTTTCTCCGTACAGACTATAATTTGTGCAATGGAAAGTTTCACAAACACCTGCAGGACCTGTTCGCC
  CCACTTGTTGTTAGATATGTGGATCTGATGGAGTCCTCCATTGCACAATCTATCCACAGGGGCTTTGAGC
  GGGAGTCATGGGAGCCAGTCAAGAGTTTAACCAGTAACCTACCCAATGTGAACCTACCCAATGTGAACCT
  ACCCAAAGTACCAAATCTACCAGTTAACATCCCTCTAGGCATCCCACAGATGCCCACTTTTTCGGCACCG
  TCATGGATGGCTGCTATATATGATGCTGATAATGGATCAGGTACATCAGAAGATTTGTTCTGGAAACTTG
  ATGCCCTTCAGACTTTCATCCGTGACCTACACTGGCCTGAGGAAGAGTTTGGAAAACACTTAGAGCAGCG
  GCTAAAACTGATGGCAAGTGACATGATTGAGTCCTGCGTCAAAAGAACCAGGATTGCATTTGAAGTTAAG
  CTGCAAAAAACCAGTCGATCAACAGATTTTCGAGTCCCACAGTCAATATGCACCATGTTTAATGTTATGG
  TTGATGCCAAAGCTCAATCAACAAAACTTTGCAGCATGGAAATGGGCCAAGAGCATCAATACCATTCAAA
  AATAGATGAACTAATTGAAGAAACCGTTAAAGAAATGATCACACTTTTGGTGGCAAAGTTTGTAACTATA
  TTGGAAGGCGTACTGGCAAAGTTATCCAGATATGACGAGGGGACTTTGTTTTCTTCGTTTCTGTCATTCA
  CGGTGAAGGCAGCTTCCAAGTATGTGGACGTGCCTAAACCCGGGATGGACGTGGCCGACGCCTACGTGAC
  TTTCGTCCGCCATTCTCAGGATGTCCTTCGTGATAAGGTCAATGAGGAGATGTATATAGAAAGGTTATTT
  GATCAATGGTACAACAGCTCCATGAACATCATCTGCACGTGGCTGACCGACAGGATGGACCTGCAGCTCC
  ACATTTACCAGCTGAAAACCCTGATCAGGATGGTAAAGAAAACCTATAGAGATTTCCGATTGCAAGGGGT
  CCTGGATTCCACCTTAAACAGCAAGACCTATGAGACCATCCGAAACCGTCTCACTGTGGAGGAAGCTACA
  GCGTCAGTGAGTGAGGGTGGGGGCTTACAAGGTATCAGCATGAAGGACAGTGATGAGGAAGATGAAGAAG
  ATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224888 representing NM_001042617
  Red=Cloning site Green=Tags(s)
  
  MLDPSSSEEESDEILEEERGKDVLGSAASGARLSPSRTSEGSAGSAGMGGSGAGAGVGAGGGGGSGASSG
  GGAGGLQPSSRAGGGRPSSPSPSVVSEKEKEELERLQKEEEERKKRLQLYVFVMRCIAYPFNAKQPTDMA
  RRQQKISKQQLQTVKDRFQAFLNGETQIVADEAFMNAVQSYYEVFLKSDRVARMVQSGGCSANDSREVFK
  KHIEKRVRSLPEIDGLSKETVLSSWMAKFDAIYRGEEDPRKQQARMTASAASELILSKEQLYEMFQNILG
  IKKFEHQLLYNACQLDNPDEQAAQIRRELDGRLQMADQIARERKFPKFVSKEMENMYIEELKSSVNLLMA
  NLESMPVSKGGEFKLQKLKRSHNASIIDMGEESENQLSKSDVLLSFSLEVVIMEVQGLKSLAPNRIVYCT
  MEVEGGEKLQTDQAEASKPTWGTQGDFSTTHALPAVKVKLFTESTGVLALEDKELGRVILHPTPNSPKQS
  EWHKMTVSKNCPDQDLKIKLAVRMDKPQNMKHSGYLWTIGKNVWKRWKKRFFVLVQVSQYTFAMCSYREK
  KAEPQELLQLDGYTVDYTDPQPGLEGGRAFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSHKPVPP
  TQVQKLNAKGGNVPQLDAPISQFSGLKDADRAQKHGMDEFISSNPCNFDHASLFEMVQRLTLDHRLNDSY
  SCLGWFSPGQVFVLDEYCARNGVRGCHRHLCYLRDLLERAENGAMIDPTLLHYSFAFCASHVHGNRPDGI
  GTVTVEEKERFEEIKERLRVLLENQITHFRYCFPFGRPEGALKATLSLLERVLMKDIVTPVPQEEVKTVI
  RKCLEQAALVNYSRLSEYAKIEENQKDAENVGRLITPAKKLEDTIRLAELVIEVLQQNEEHHAEPHVDKG
  EAFAWWSDLMVEHAETFLSLFAVDMDAALEVQPPDTWDSFPLFQLLNDFLRTDYNLCNGKFHKHLQDLFA
  PLVVRYVDLMESSIAQSIHRGFERESWEPVKSLTSNLPNVNLPNVNLPKVPNLPVNIPLGIPQMPTFSAP
  SWMAAIYDADNGSGTSEDLFWKLDALQTFIRDLHWPEEEFGKHLEQRLKLMASDMIESCVKRTRIAFEVK
  LQKTSRSTDFRVPQSICTMFNVMVDAKAQSTKLCSMEMGQEHQYHSKIDELIEETVKEMITLLVAKFVTI
  LEGVLAKLSRYDEGTLFSSFLSFTVKAASKYVDVPKPGMDVADAYVTFVRHSQDVLRDKVNEEMYIERLF
  DQWYNSSMNIICTWLTDRMDLQLHIYQLKTLIRMVKKTYRDFRLQGVLDSTLNSKTYETIRNRLTVEEAT
  ASVSEGGGLQGISMKDSDEEDEEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042617
• ORF Size: 4065 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042617.1, NP_001036082.1
• RefSeq Size: 5475 bp
• RefSeq ORF: 4068 bp
• Locus ID: 27062
• UniProt ID: Q80TJ1
• Cytogenetics: 14 A1
• MW: 153.6 kDa
• Gene Summary: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates catecholamine loading of DCVs. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles by acting as a PtdIns(4,5)P2-binding protein that acts at prefusion step following ATP-dependent priming and participates in DCVs-membrane fusion. However, it may also participate in small clear synaptic vesicles (SVs) exocytosis and it is unclear whether its function is related to Ca(2+) triggering (By similarity).[UniProtKB/Swiss-Prot Function]