Fis1 (NM_001163243) Mouse Tagged ORF Clone
CAT#: MR224358
- TrueORF®
Fis1 (Myc-DDK-tagged) - Mouse fission 1 (mitochondrial outer membrane) homolog (yeast) (Fis1), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001163243" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Fis1 |
Synonyms | 2010003O14Rik; Ttc11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224358 representing NM_001163243
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGAGACGAAGCTGCAAGGAATTTTGAAAGGAAATTTCAGTCTGAGCAGGCAGCTGGTTCTGTGT CCAAGAGCACGCAATTTGAATATGCCTGGTGCCTGGTTCGAAGCAAATACAATGAGGACATCCGCAGAGG CATCGTGCTGCTGGAGGAGCTGTTGCCCAAAGGGAGCAAAGAGGAACAGCGGGACTATGTCTTCTACCTG GCCGTGGGCAACTACCGGCTCAAGGAATATGAAAAGGCTCTAAAGTATGTGCGAGGGCTGTTGCAGACTG AGCCCCAGAACAACCAGGCCAAGGAGCTGGAACGCCTGATTGATAAGGCCATGAAGAAAGATGGACTGGT AGGCATGGCCATCGTTGGTGGCATGGCCCTGGGCGTGGCAGGCCTGGCTGGACTCATTGGACTGGCTGTC TCCAAGTCCAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224358 representing NM_001163243
Red=Cloning site Green=Tags(s) MPRDEAARNFERKFQSEQAAGSVSKSTQFEYAWCLVRSKYNEDIRRGIVLLEELLPKGSKEEQRDYVFYL AVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAV SKSKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163243 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163243.1, NP_001156715.1 |
RefSeq Size | 966 bp |
RefSeq ORF | 438 bp |
Locus ID | 66437 |
UniProt ID | Q9CQ92 |
Cytogenetics | 5 G2 |
MW | 16.7 kDa |
Gene Summary | Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. May be not essential for the assembly of functional fission complexes and the subsequent membrane scission event. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224358 | Fis1 (tGFP-tagged) - Mouse fission 1 (mitochondrial outer membrane) homolog (yeast) (Fis1) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug) |
USD 365.00 |
|
MR224358L3 | Lenti ORF clone of Fis1 (Myc-DDK-tagged) - Mouse fission 1 (mitochondrial outer membrane) homolog (yeast) (Fis1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 465.00 |
|
MR224358L4 | Lenti ORF clone of Fis1 (mGFP-tagged) - Mouse fission 1 (mitochondrial outer membrane) homolog (yeast) (Fis1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 465.00 |
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