Arhgef7 (NM_001113517) Mouse Tagged ORF Clone

CAT#: MR224123

Arhgef7 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 1

Product Images

Circular map for MR224123

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef7
• Synonyms: betaPix; betaPix-b; betaPix-c; Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224123 representing NM_001113517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCTGCCGAGCAGACCGTTACGTGGCTCATCACCCTGGGCGTGCTGGAGTCGCCCAAGAAAACCA
  TCTCGGACCCTGAGGTCTTCTTGCAGGCGTCGCTCAAGGACGGGGTGGTCCTATGCCGGCTGCTGGAGCG
  CCTGCTGCCCGGGACCATCGAGAAAGTCTACCCGGAGCCGCGGAACGAGAGCGAGTGCCTGAGCAACATC
  CGCGAGTTCCTGCGCGCCTGCGGGGCGTCCCTGCGCCTAGAGACTTTTGATGCAAATGATTTGTATCAGG
  GGCAGAACTTTAACAAGGTCCTCAGCTCCTTGGTGACACTAAATAAAGTGACAGCAGACATTGGACTAGG
  AAGTGATTCTGTGTGTGCTCGGCCTTCGTCCCATCGGATAAAGTCTTTTGATTCCCTGGGATCCCAGTCT
  TCACACAGTAGGACTTCAAAATTGCTTCAGAGCCAGTACCGAAGCTTGGACATGACTGATAACACCAACA
  GCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGG
  TGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCACACACAATGGCAGGACCGGC
  TGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCT
  TGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTGGTGCTACAGAA
  CATCCTGGAAACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTCCACCTACCTGCGGCCACTG
  CAGACCAGTGACAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAATCTAGAGGAAATATCTTCCT
  TCCAGCAAGTGCTTGTACAGTCCCTAGAAGAATGCACGAAGTCTCCTGAAGCCCAACAGAGAGTTGGTGG
  CTGCTTCCTGAGCCTGATGCCGCAGATGAGGACCCTGTACCTCGCTTACTGTGCCAACCACCCATCTGCT
  GTGAGCGTCCTCACAGAGCACAGTGAGGACCTAGGAGAGTTCATGGAAACAAAAGGTGCCAGCAGCCCTG
  GGATCCTGGTGCTGACCACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACAAGTACCCCACACTGCTAAA
  GGAGCTGGAGAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCAGAAGTCTATGACGGCCTTC
  AAAAACCTTTCAGCTCAGTGTCAAGAAGTTCGCAAGAGGAAGGAGCTGGAGCTGCAGATCCTGACGGAGC
  CCATCAGGAGCTGGGAGGGGGATGACATAAAGACCCTGGGCAGTGTCACATACATGTCCCAAGTCACCAT
  TCAGTGTGCGGGAAGCGAGGAGAAGAATGAGAGATACCTCCTGCTCTTCCCAAACCTTCTGCTCATGTTG
  TCTGCAAGTCCCAGGATGAGTGGTTTCATCTATCAGGGGAAGCTGCCAACAACAGGAATGACAATCACAA
  AGCTTGAGGACAGTGAAAACCATAGGAATGCATTTGAGATATCAGGGAGCATGATCGAGCGGATTCTGGT
  GTCCTGCACCAGCCAGCAGGACTTACACGAGTGGGTGGAACACCTGCAGAAGCAGACGAAGGTCACATCT
  GTGAGCAACCCCACCATCAAACCCCACTCGGTGCCATCACACACACTTCCTTCCCATCCTCTCACTCCAT
  CCAGCAAACACGCGGACAGCAAGCCCGTGGCACTGACGCCTGCGTACCACACACTCCCCCACCCCTCTCA
  CCATGGCACCCCACACACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAAGACCCCCAAGCCTTGGAGC
  CTGAGTTGCCTGCGGCCTGCACCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTCAGTA
  AGAGCCCCAAGACCATGAAAAAGCTGCTGCCGAAGCGCAAGCCCGAGCGGAAGCCTTCGGACGAGGAGTT
  CGCTGTGCGCAAGAGCACAGCGGCGCTGGAAGAAGACGCTCAGATCCTGAAGGTTATCGAAGCTTATTGC
  ACAAGTGCAAAGACGCGCCAGACCCTGAACTCAACATGGCAAGGCACTGACCTGATGCATAATCACGTCT
  TGGCTGATGACGACCAATCAAGTCTAGACTCCTTGGGTCGTCGCAGTAGCCTTTCTCGTTTGGAGCCCTC
  AGACCTCTCGGAAGACTCTGAGTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTGCATCC
  CGTAAGAGCTGCTGCTCATATATCTCTCACCAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224123 representing NM_001113517
  Red=Cloning site Green=Tags(s)
  
  MNSAEQTVTWLITLGVLESPKKTISDPEVFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRNESECLSNI
  REFLRACGASLRLETFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQS
  SHSRTSKLLQSQYRSLDMTDNTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTG
  WFPSNYVREIKPSEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTYLRPL
  QTSDKLSSANTSYLMGNLEEISSFQQVLVQSLEECTKSPEAQQRVGGCFLSLMPQMRTLYLAYCANHPSA
  VSVLTEHSEDLGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRQDIQKSMTAF
  KNLSAQCQEVRKRKELELQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNERYLLLFPNLLLML
  SASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCTSQQDLHEWVEHLQKQTKVTS
  VSNPTIKPHSVPSHTLPSHPLTPSSKHADSKPVALTPAYHTLPHPSHHGTPHTTISWGPLEPPKTPKPWS
  LSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFAVRKSTAALEEDAQILKVIEAYC
  TSAKTRQTLNSTWQGTDLMHNHVLADDDQSSLDSLGRRSSLSRLEPSDLSEDSEYDSIWTAHSYRMGSAS
  RKSCCSYISHQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113517
• ORF Size: 2346 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001113517.2
• RefSeq Size: 4946 bp
• RefSeq ORF: 2349 bp
• Locus ID: 54126
• UniProt ID: Q9ES28
• Cytogenetics: 8 A1.1
• MW: 88.1 kDa
• Gene Summary: Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function]