Pou2f2 (NM_001163556) Mouse Tagged ORF Clone
CAT#: MR224033
- TrueORF®
Pou2f2 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001163556" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pou2f2 |
Synonyms | Oct-2; Oct2a; Oct2b; Oct2c; Oct2d; Otf-2; Otf2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224033 representing NM_001163556
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCATTCCAGCATGGGGGCTCCAGAAATAAGAATGTCTAAGCCCCTGGAGGCCGAGAAGCAAAGTC TGGACTCCCCGTCAGAGCACACAGACACCGAAAGAAATGGACCCGACATTAACCATCAGAACCCCCAGAA TAAAGCGTCCCCATTCTCTGTGTCCCCAACTGGCCCCAGCACCAAGATCAAGGCTGAAGACCCCAGTGGC GATTCAGCCCCAGCAGCACCCCCGCCCCCCCAGCCGGCTCAGCCTCATCTGCCCCAGGCCCAACTCATGC TGACGGGCAGCCAGCTAGCTGGGGACATACAGCAACTCCTCCAGCTCCAGCAGCTGGTGCTTGTCCCCGG CCACCACCTCCAGCCACCTGCTCAGTTCCTGCTGCCACAGGCACAGCAGAGTCAGCCAGGCCTGCTACCA ACGCCAAATCTATTCCAGCTACCTCAACAAACCCAGGGAGCTCTCCTGACCTCCCAGCCCCGGGCTGGGC TTCCTACACAGCCCCCGAAATGCTTGGAGCCGCCCTCCCACCCGGAGGAGCCCAGCGATCTGGAGGAGCT GGAACAGTTTGCTCGCACCTTCAAGCAACGCCGCATCAAGCTGGGCTTCACACAGGGTGATGTGGGCCTG GCCATGGGCAAGCTCTATGGCAACGACTTCAGCCAAACGACCATTTCCCGCTTCGAGGCCCTCAACCTGA GCTTCAAGAACATGTGTAAACTCAAGCCCCTCCTGGAGAAGTGGCTCAACGACGCAGAGACTATGTCTGT GGATTCAAGCCTACCCAGCCCAAACCAGCTGAGCAGCCCCAGCCTGGGTTTCGACGGGCTGCCGGGGCGG AGACGCAAGAAGAGGACCAGCATCGAGACGAATGTCCGCTTCGCCTTAGAGAAGAGTTTCCTAGCGAACC AGAAGCCTACCTCAGAGGAGATCCTGCTGATCGCAGAGCAGCTGCACATGGAGAAGGAAGTGATCCGCGT CTGGTTCTGCAACCGGCGCCAGAAGGAGAAACGCATCAACCCTTGCAGTGCGGCCCCCATGCTGCCCAGC CCGGGAAAGCCGACCAGCTACAGCCCTCACCTGGTCACACCCCAAGGGGGCGCAGGGACCTTACCATTGT CCCAAGCTTCTAGCAGTCTGAGCACAACAGTTACTACCTTATCCTCAGCTGTGGGGACGCTCCATCCCAG CCGGACAGCAGGAGGGGGTGGGGGTGGGGGCGGAGCTGCGCCCCCCCTCAATTCCATCCCCTCTGTCACT CCCCCACCCCCGGCCACCACCAACAGCACAAACCCGAGCCCTCAAGGCAGCCACTCGGCTATTGGCTTGT CGGGCCTGAACCCCAGCGCGGGCCCTGGCCTCTGGTGGAACCCTGCCCCTTACCAGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224033 representing NM_001163556
Red=Cloning site Green=Tags(s) MVHSSMGAPEIRMSKPLEAEKQSLDSPSEHTDTERNGPDINHQNPQNKASPFSVSPTGPSTKIKAEDPSG DSAPAAPPPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLP TPNLFQLPQQTQGALLTSQPRAGLPTQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGL AMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGR RRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPS PGKPTSYSPHLVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVT PPPPATTNSTNPSPQGSHSAIGLSGLNPSAGPGLWWNPAPYQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163556 |
ORF Size | 1389 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163556.1, NP_001157028.1 |
RefSeq Size | 3249 bp |
RefSeq ORF | 1392 bp |
Locus ID | 18987 |
UniProt ID | Q00196 |
Cytogenetics | 7 13.73 cM |
MW | 49.9 kDa |
Gene Summary | Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform OCT2.5 activates the U2 small nuclear RNA (snRNA) promoter. Isoforms OCT2.1, OCT2.2 and OCT2.3 activate octamer-containing promoters. Isoforms OCT2.4 and OCT2.5 repress some promoters and activate others. Isoform OCT2.7 is unable to bind to the octamer motif, but can still activate the beta-casein gene promoter at low levels.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216281 | Pou2f2 (untagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1, (10ug) |
USD 457.00 |
|
MG224033 | Pou2f2 (tGFP-tagged) - Mouse POU domain class 2 transcription factor 2 (Pou2f2) transcript variant 1, (10ug) |
USD 703.00 |
|
MR224033L3 | Lenti ORF clone of Pou2f2 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1 |
USD 803.00 |
|
MR224033L4 | Lenti ORF clone of Pou2f2 (mGFP-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1 |
USD 803.00 |
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