B3gnt2 (NM_016888) Mouse Tagged ORF Clone
CAT#: MR223657
- TrueORF®
B3gnt2 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016888" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | B3gnt2 |
Synonyms | AA408337; AA409404; AW260308; B3Galt6; B3gnt1; Beta3gnt; BGnT-1; BGnT-2; BGnT1; BGnT2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223657 representing NM_016888
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGGGGCGTCGAAGAGTCAAGTTGCTGGGCATCCTGATGATGGCAAATGTCTTCATTTATTTGA TTGTGGAAGTCTCCAAAAACAGTAGCCAAGACAAAAATGGAAAGGGAGGAGTAATAATCCCGAAAGAGAA GTTCTGGAAGCCACCCAGCACTCCCCGGGCATACTGGAACAGGGAACAGGAGAAGCTGAACAGGTGGTAC AATCCCATCTTGAACAGGGTGGCCAATCAGACAGGGGAGCTAGCCACATCTCCAAACACAAGTCACCTGA GCTATTGTGAACCAGACTCGACGGTCATGACAGCTGTGACAGATTTTAATAATCTGCCGGACAGATTTAA AGACTTTCTCTTGTATTTGAGATGCCGGAATTACTCGCTGCTTATAGATCAACCGAAGAAATGTGCAAAG AAGCCCTTCTTACTATTGGCGATAAAGTCCCTCATTCCACATTTTGCCAGAAGGCAAGCAATTCGGGAGT CTTGGGGCCGAGAAACCAACGTAGGGAACCAGACAGTAGTGAGGGTCTTCCTGTTGGGCAAGACACCCCC AGAGGACAACCACCCTGACCTTTCGGACATGCTTAAGTTTGAGAGTGACAAGCACCAGGACATCCTCATG TGGAACTATAGAGACACATTCTTCAACCTGTCCCTGAAGGAAGTGCTGTTTCTTAGGTGGGTGAGCACTT CCTGTCCAGACGCAGAGTTTGTCTTCAAGGGCGATGATGACGTGTTTGTGAACACCCATCACATCCTTAA TTACTTGAATAGCTTATCCAAGAGCAAAGCCAAAGACTTGTTCATAGGTGACGTGATCCACAATGCTGGG CCTCACCGGGATAAGAAACTGAAGTACTACATCCCAGAAGTCTTCTACACCGGCGTCTACCCACCGTATG CCGGGGGTGGTGGATTCCTGTACTCCGGCCCCCTTGCCTTGAGGCTGTACAGTGCGACTAGCCGGGTCCA TCTCTACCCTATTGATGATGTTTATACGGGAATGTGCCTTCAGAAACTGGGCCTTGTTCCAGAGAAGCAC AAAGGCTTCAGGACATTTGATATTGAAGAGAAAAATAAGAAAAATATTTGTTCCTATATAGACCTAATGT TAGTACATAGCAGAAAACCTCAAGAGATGATTGATATCTGGTCTCAGTTGCAAAGTCCTAATTTAAAATG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223657 representing NM_016888
Red=Cloning site Green=Tags(s) MSVGRRRVKLLGILMMANVFIYLIVEVSKNSSQDKNGKGGVIIPKEKFWKPPSTPRAYWNREQEKLNRWY NPILNRVANQTGELATSPNTSHLSYCEPDSTVMTAVTDFNNLPDRFKDFLLYLRCRNYSLLIDQPKKCAK KPFLLLAIKSLIPHFARRQAIRESWGRETNVGNQTVVRVFLLGKTPPEDNHPDLSDMLKFESDKHQDILM WNYRDTFFNLSLKEVLFLRWVSTSCPDAEFVFKGDDDVFVNTHHILNYLNSLSKSKAKDLFIGDVIHNAG PHRDKKLKYYIPEVFYTGVYPPYAGGGGFLYSGPLALRLYSATSRVHLYPIDDVYTGMCLQKLGLVPEKH KGFRTFDIEEKNKKNICSYIDLMLVHSRKPQEMIDIWSQLQSPNLKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016888 |
ORF Size | 1191 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016888.5, NP_058584.3 |
RefSeq Size | 2699 bp |
RefSeq ORF | 1194 bp |
Locus ID | 53625 |
UniProt ID | Q9Z222 |
Cytogenetics | 11 14.22 cM |
MW | 46.3 kDa |
Gene Summary | Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains (PubMed:9892646). Probably constitutes the main polylactosamine synthase (PubMed:17890318).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200263 | B3gnt2 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 1, (10ug) |
USD 457.00 |
|
MG223657 | B3gnt2 (tGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-13-N-acetylglucosaminyltransferase 2 (B3gnt2) transcript variant 1, (10ug) |
USD 657.00 |
|
MR223657L3 | Lenti ORF clone of B3gnt2 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 1 |
USD 757.00 |
|
MR223657L4 | Lenti ORF clone of B3gnt2 (mGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 1 |
USD 757.00 |
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