Rnf152 (NM_178779) Mouse Tagged ORF Clone
CAT#: MR223504
- TrueORF®
Rnf152 (Myc-DDK-tagged) - Mouse ring finger protein 152 (Rnf152), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_178779" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rnf152 |
Synonyms | A930029B02Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223504 representing NM_178779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACACTCTCCCAGGATTCCCTGTTGGAATGTCAGATCTGTTTTAATTATTACAGCCCCCGACGAA GGCCCAAGTTGTTGGATTGCAAGCACACCTGCTGCTCGGTGTGCCTCCAGCAGATGAGGACCAGCCAGAA GGACGTAAGGTGCCCCTGGTGCCGTGGCATCACCAAATTGCCCCCGGGCTTCTCTGTATCACAACTGCCC GATGACCCAGAGGTCTTGGCAGTCATTGCCATACCACACACTTCCGAGCATACCCCAGTCTTCATCAAAC TTCCAAGCAATGGGTGCTACATGCTGCCCCTGCCCATCTCTAAGGAGCGTACACTCCTGCCGGGAGACAT GGGCTGCCGCCTGCTGCCAGGGAGCCAGCAGAAGTCTCTCACCGTGGTGACCATCCCTGCAGAACAGCAG CCCCTGCAGGGTGGAGCTCCCCCGGAGGCTGTGGAGGAGGAGCCTGACAGAAGGGGCGTGGTGAAGAGCT CCACATGGTCTGGCGTGTGCACTGTCATCCTGGTGGCCTGTGTTTTGGTCTTCCTCCTGGGCATTGTGCT ACACAACATGTCCTGCATCTCTAAGCGCTTCACTGTGATATCCTGTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223504 representing NM_178779
Red=Cloning site Green=Tags(s) METLSQDSLLECQICFNYYSPRRRPKLLDCKHTCCSVCLQQMRTSQKDVRCPWCRGITKLPPGFSVSQLP DDPEVLAVIAIPHTSEHTPVFIKLPSNGCYMLPLPISKERTLLPGDMGCRLLPGSQQKSLTVVTIPAEQQ PLQGGAPPEAVEEEPDRRGVVKSSTWSGVCTVILVACVLVFLLGIVLHNMSCISKRFTVISCG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178779 |
ORF Size | 609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178779.4, NP_848894.1 |
RefSeq Size | 8529 bp |
RefSeq ORF | 612 bp |
Locus ID | 320311 |
UniProt ID | Q8BG47 |
Cytogenetics | 1 E2.1 |
MW | 22.8 kDa |
Gene Summary | E3 ubiquitin-protein ligase mediating 'Lys-63'-linked polyubiquitination of RRAGA in response to amino acid starvation. Thereby, regulates mTORC1 signaling and plays a role in the cellular response to amino acid availability (PubMed:25936802). Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214454 | Rnf152 (untagged) - Mouse ring finger protein 152 (Rnf152), transcript variant 1, (10ug) |
USD 330.00 |
|
MG223504 | Rnf152 (tGFP-tagged) - Mouse ring finger protein 152 (Rnf152) transcript variant 1, (10ug) |
USD 500.00 |
|
MR223504L3 | Lenti ORF clone of Rnf152 (Myc-DDK-tagged) - Mouse ring finger protein 152 (Rnf152), transcript variant 1 |
USD 600.00 |
|
MR223504L4 | Lenti ORF clone of Rnf152 (mGFP-tagged) - Mouse ring finger protein 152 (Rnf152), transcript variant 1 |
USD 600.00 |
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