Acin1 (NM_019567) Mouse Tagged ORF Clone

CAT#: MR223236

Acin1 (Myc-DDK-tagged) - Mouse apoptotic chromatin condensation inducer 1 (Acin1), transcript variant 1

Product Images

Circular map for MR223236

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acin1
• Synonyms: 2610036I19Rik; 2610510L13Rik; Acinus; acinusL; acinusS; Acn; C79325; mKIAA0670
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223236 representing NM_019567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCGGCTGATCGCTGCCGCTTTACCAATACAATAGAGTCTGCTTACTACCAGCAACCTGGCCCTC
  TTCCTCCTCTTCCTCCAGAGAGACCAGTCCAGCCGAACTCGGGGTTTGCCAAAGGGATAAACAGCACTTC
  CAGGGGGAGAAAAAAAATAATGATGTTCTCAGACAGCAGAGCAGGTGAAGAGAAGGAGGAAGTGACCATG
  GACACCAGTGAAAACAGACCTGAAAATGAGGTGCCTGAGCCTCCTCTGCCTGTTGCAGACCAAGTCAGCA
  ATGATGAGCGCCCAGAGGGTGGTGCTGAAGAAGAGGAAAAGAAAGAGAGTTCGATGCCCAAGTCATTCAA
  GAGGAAAATCTCCGTTGTCTCAGCTACCAAGGGGGTGCAAGCTGGAAACAGTGACACAGAGGGGGGCCAG
  CCTGGCCGAAAACGCCGTTGGGGAGCCAGCACTGCCGCGACACAGAAGAAACCGTCCATCAGTATCACCA
  CTGAGTCACTCAAGAGCCTCATCCCCGACATCAAACCCCTGGCGGGGCAGGAGGCTGTTGTGGATCTTCA
  TGCCGATGACTCCCGAATCTCTGAGGATGAGACAGAGCGTAATGGCGACGATGGGACCCATGACAAGGGA
  CTGAAGATATGCCGGACAGTCACTCAGGTAGTACCCGCAGAGGGCCAGGAGAATGGGCAGAGGGAAGAGG
  AAGAAGAGAAAGAGCCTGAAGCCGAGCTGCCGGCGCCACCCCAGGTGTCAGTGGAGGTTGCCTTGCCCCC
  ACCTGTGGAGCACGAAGTAAAGAAAGTAACATTAGGAGATACCTTAACCCGGAGGTCCATCAGCCAACAG
  AAGTCTGGAGTTTCCATTACAATTGATGACCCAGTCCGGACCGCCCAGGTGCCCTCCCCACCCAGGGGCA
  AGATCAGTAACATTGTCCACATCTCCAACCTGGTTCGTCCCTTCACTTTAGGCCAGCTGAAGGAATTATT
  GGGGCGTACAGGAACTTTGGTGGAAGAGGCCTTCTGGATAGACAAGATCAAATCTCATTGCTTTGTGACG
  TACTCTACAGTAGAGGAAGCCGTTGCCACCCGCACAGCTCTGCACGGGGTCAAGTGGCCCCAGTCCAACC
  CCAAATTCCTTTGTGCTGACTATGCTGAGCAAGATGAGCTGGACTATCACCGGGGACTCTTGGTAGATCG
  GCCATCTGAAACTAAGGCAGAGGAACAGGGAGCACCAAGGCCCCTGCATCCCCCACCCCCACCCCCAGTC
  CAGCCACCGCCCCACCCCCGGGCTGAGCAGCGGGAGCAGGAAAGGGCTGTTCGAGAGCAATGGGCAGAAC
  GGGAACGGGAAATGGAGCGCCGGGAGAGGACTCGGTCTGAGAGAGAATGGGATCGGGACAAAGTTCGAGA
  GGGACCCCGCTCCCGATCACGGTCCCGTGACCGCCGCCGGAAAGAGCGAGCAAAATCTAAAGAAAAGAAG
  AGTGAAAAGAAAGAAAAAGCCCAGGAGGAGCCACCTGCCAAGCTGCTGGATGACCTCTTCCGTAAGACTA
  AGGCAGCTCCCTGCATCTATTGGCTCCCTCTGACTGAGAGCCAAATTGTTCAGAAGGAGGCAGAGCAAGC
  TGAACGGGCCAAGGAGCGGGAGAAGCGGCGAAAAGAACGAGAAGAAGAAGAACAAAAGGAACGGGAGAAG
  GAAGCTGAGCGGGAACGGAACCGGCAGCTAGAACGGGAGAAGAGGAGGGAGCACAGCAGGGAGAGAGAGA
  GGGACAGGGAGAGAGAGCGGGACAGGGGTGACCGAGAGCGGGAGAGGGAGCGAGACCGAGACCGAGGCAG
  GGAGAGGGATCGCAGAGACACCAAGCGCCACAGCAGAAGCCGGAGTCGAAGCACACCTGTACGGGACCGG
  GGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223236 representing NM_019567
  Red=Cloning site Green=Tags(s)
  
  MSPADRCRFTNTIESAYYQQPGPLPPLPPERPVQPNSGFAKGINSTSRGRKKIMMFSDSRAGEEKEEVTM
  DTSENRPENEVPEPPLPVADQVSNDERPEGGAEEEEKKESSMPKSFKRKISVVSATKGVQAGNSDTEGGQ
  PGRKRRWGASTAATQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKG
  LKICRTVTQVVPAEGQENGQREEEEEKEPEAELPAPPQVSVEVALPPPVEHEVKKVTLGDTLTRRSISQQ
  KSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVT
  YSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKAEEQGAPRPLHPPPPPPV
  QPPPHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKK
  SEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTESQIVQKEAEQAERAKEREKRRKEREEEEQKEREK
  EAERERNRQLEREKRREHSRERERDRERERDRGDRERERERDRDRGRERDRRDTKRHSRSRSRSTPVRDR
  GGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019567
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019567.3, NP_062513.3
• RefSeq Size: 2519 bp
• RefSeq ORF: 1905 bp
• Locus ID: 56215
• UniProt ID: Q9JIX8
• Cytogenetics: 14 C2
• MW: 73.5 kDa
• Gene Summary: Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]