Wnt16 (NM_053116) Mouse Tagged ORF Clone

CAT#: MR222522

Wnt16 (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 16 (Wnt16)

Product Images

Circular map for MR222522

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt16
• Synonyms: E130309I19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222522 representing NM_053116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGAGCGGCGCTCCTGGCCCTGCCCAGCTTGTGTGCGCTGTGGGCAGCCGTGCTGTCGCTGCTCC
  CCTGCGGAACCCAGGGCAACTGGATGTGGTTGGGCATCGCCTCTTTCGGGGTACCGGAGAAGCTGGGCTG
  CGCCGACTTGCCGCTGAACAGCCGCCAGAAGGAGCTGTGCAAGAGGAAACCGTACCTGCTGCCTAGCATC
  CGCGAGGGCGCCAGGCTGGGCATTCAGGAGTGCAGAAGCCAGTTCCGACACGAGAGGTGGAACTGTATGG
  TCGCCACTACCACTTCCACCCAGCTCGCCACAGCCCCCCTCTTTGGCTATGAGCTGAGTAGCGGCACCAA
  GGAGACAGCATTCATTTATGCCATCATGGCAGCGGGCCTGGTGCACTCTGTCACCAGGTCATGCAGTGCA
  GGCAACATGACCGAATGTTCCTGTGACACCACCTTGCAGAATGGTGGCTCACCAAGTGAAGGCTGGCACT
  GGGGAGGATGCTCGGATGATGTCCAGTACGGCATGTGGTTCAGCAGAAAGTTTCTAGATCTTCCCATCAG
  AAACACCACAGGAAAAGAAAGCAGAGTCCTGCTAGCCATGAATCTACACAACAACGAAGCGGGGCGGCAG
  GCTGTCGCCAAGTTAATGTCTGTGGACTGCCGCTGCCACGGAGTTTCCGGCTCCTGTGCTGTGAAAACCT
  GCTGGAAAACTATGTCTTCTTTTGAAAAGATTGGGCATTTTTTAAAGGATAAATATGAAAACAGCATCCA
  GATCTCAGACAAAACCAAGAGGAAAATGCGCAGGAGAGAAAAAGACCAGAGGCAGACCCCCATTCTCAAG
  GATGACTTGCTGTACGTTCATAAGTCTCCCAACTACTGCGTGGAGAACAAGAAACTGGGGATTCCTGGGA
  CCCAGGGCAGAGAGTGCAACCGGACATCAGGAGGCGCAGATGGCTGTAACCTCCTCTGCTGTGGCCGAGG
  CTACAACACCCATGTAGTCAGGCACGTGGAGAGGTGTGAGTGTAAGTTTATCTGGTGCTGCTACGTCCGC
  TGCAGGAGGTGTGAAAGTATGACCGATGTCCACACGTGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222522 representing NM_053116
  Red=Cloning site Green=Tags(s)
  
  MDRAALLALPSLCALWAAVLSLLPCGTQGNWMWLGIASFGVPEKLGCADLPLNSRQKELCKRKPYLLPSI
  REGARLGIQECRSQFRHERWNCMVATTTSTQLATAPLFGYELSSGTKETAFIYAIMAAGLVHSVTRSCSA
  GNMTECSCDTTLQNGGSPSEGWHWGGCSDDVQYGMWFSRKFLDLPIRNTTGKESRVLLAMNLHNNEAGRQ
  AVAKLMSVDCRCHGVSGSCAVKTCWKTMSSFEKIGHFLKDKYENSIQISDKTKRKMRRREKDQRQTPILK
  DDLLYVHKSPNYCVENKKLGIPGTQGRECNRTSGGADGCNLLCCGRGYNTHVVRHVERCECKFIWCCYVR
  CRRCESMTDVHTCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053116
• ORF Size: 1092 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053116.4, NP_444346.3
• RefSeq Size: 1666 bp
• RefSeq ORF: 1095 bp
• Locus ID: 93735
• UniProt ID: Q9QYS1
• Cytogenetics: 6 A3.1
• MW: 41.2 kDa
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).[UniProtKB/Swiss-Prot Function]