Ptprj (NM_008982) Mouse Tagged ORF Clone

CAT#: MR221777

Ptprj (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1

Product Images

Circular map for MR221777

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptprj
• Synonyms: AI450271; BET; Byp; CD148; DEP-1; Ptpb2; PTPbeta2; RPTPJ; Scc-1; Scc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221777 representing NM_008982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCGCGGAGGAGGCAGAGGGAGCAGCCGGGGCCGCGGGAGCCGGGAGCTGGGAGCCACGCGCGGAG
  GGTGGGCGCCGCTCGCTCCGCCCCGCGAAGCCCCTGCGAGTCTAAGGCCGCGGCCGCTCCGCGCCAGGCG
  CGCTAGGCTCCGCCGTGTGGCCGCCGCCGCCGCCGCTGCCATGTCCCCGGGGAAGCCCGGGGCGGGCGGA
  GCGGGGACTAGGCGGACCGGCTGGCGGAGAAGGAGGCGGAGGCGTCGGCTGGAGACGGAGACGAGGGCGC
  CCGGCTTCGGGCACACGGCGGGGCGCGTCCCGGGCACGTTCCAGGGCGCGCAGGGCATGAAGCCCGCGGC
  GCGGGAGACGCGGACACCCCCGCGCTCGCCCGGGCTCCGCTGGGCGCTGCTGCCGCTGCTGCTGTTGCTA
  CGCCAGGGCCAGGTCCTGTGCGCAGGTGCGGCACCGAATCCTATATTTGACATTGAAGCTGTCGTCAGCC
  CAACTAGTGTGTTATTAACATGGAAGCACAATGACTCAGGCGCTTCAGAATGTAGAATAGAGAATAAGAT
  GGAGAGCAATCTGACGTTTCCTGTTAAAAACCAGACATCATGTAACATTACAGGCTTAAGCCCAGGCACT
  TCGTATACATTCTCCATCATCTCTGTAACAACCAATGAGACCTTGAACAAAACTATCACAACAGAGCCCT
  GGCCAGTGTCTGATCTCCATGTCACCTCTGTGGGTGTGACACAGGCTCGTCTCACCTGGAGCAATGCAAA
  TGGCACTGCCTCCTACCGGATGCTGATTGAAGAGTTGACCACACATTCCTCAGTCAATATTTCAGGTCTG
  AAGCCGGGGACCAATAATAGCTTCGCTTTCCCAGAATCAAATGAGACACAGGCTGACTTTGCAGTTGCAG
  AGGAGGTCCCGGATGCCAATGGTACCAAGAGAATCCCAGTGACCAACCTATCCCAACTACACAAGAATTC
  TCTTGTCTCTGTGGACCCACCCTCTGGCCAGGATCCCTCCCTCACAGAGATCTTGCTTACTGACCTAAAG
  CCTGATACTCAGTACAATGCCACCATCTATTCTCAAGCAGCAAATGGCACTGAAGGACAGCCCAGGAACA
  AAGTGTTTAAAACAAATTCCACCCAGGTTTCTGACGTCCGAGCTATGAACATCAGTGCCTCAAGCATGAC
  CCTGACCTGGAAAAGCAATTACGATGGGTCCCGTACTTCAATTGTCTACAAAATACACGTGGCTGGGGGG
  ACCCACTCCGTCAACCAAACTGTCAATAAGACTGAGGCCATCATCCTCGGACTCAGCTCCAGCACCTTGT
  ACAACATCACAGTTCATCCTTTCCTGGGTCAGACGGAGGGCACACCAGGCTTCCTCCAAGTGTACACTTC
  CCCCGATCAGGTCTCTGACTTCCGAGTGACAAATGTCAGCACAAGGGCAATTGGTTTGGCTTGGAGGAGC
  AATGACTCCAAGTCCTTCGAGATTTTCATCAAGCAGGACGGAGGTGAGAAGCATCGAAATGCTTCGACGG
  GAAACCAGAGCTATATGGTTGAAGATTTAAAGCCTGGAACCAGTTACCATTTTGAGATAATTCCACGAGG
  ACCAGACGGGACAGAAGGGCTGTCCAGTACAGTGAATGGGAGCACTGACCCCAGTGCTGTGACTGACATC
  CGGGTGGTCAACATTAGCACCACTGAAATGCAGTTGGAGTGGCAGAATACGGACGATGCCTCTGGATACA
  CTTACCATTTAGTTCTAGAGTCTAAAAGTGGCTCCATCATCAGGACCAACAGTTCTCAGAAGTGGATCAC
  AGTAGGGAGCCTCACCCCAGGCACCTTATACAATGTCACAATCTTTCCAGAAGTGGACCAGATCCAGGGA
  ATCTCCAACTCCATTACCCAGTACACACGGCCCAGCAGTGTGTCCCACATTGAAGTAAACACCACCACCA
  CCACGGCAGCCATCCGATGGAAGAACGAGGACGCAGCCTCTGCTTCCTATGCCTACTCCGTCCTTATCTT
  GAAGACTGGAGATGGCAGCAATGTAACCAGCAACTTCACAAAAGACCCTTCTATTCTAATCCCTGAGTTA
  ATCCCTGGTGTCTCTTACACAGTGAAGATCCTTACACAAGTTGGGGATGGTACAACATCACTGGTACCTG
  GTTGGAATCTGTTCTGTACGGAACCTGAACCAGTGACCTCCTTCCACTGTGAAGTGGTCCCTAAGGAGCC
  AGCATTGGTTCTCAAGTGGGCCTGCCCCTTTGGCATGTACACAGGCTTCGAGCTGGGGGTCAGGAGTGAT
  TCCTGGGACAATATGACACGCCTAGAGAACTGCACTTCGGATGATGACACAGAGTGCAGGACGGAAGTCG
  CCTATTTGAATTTTTCTACCTCGTACAACATCAGCATCGCCACCTTGTCATGTGGGAAGATGGCGCTTCC
  CGCCCAGAACATCTGCACCACTGGCATCACAGACCCACCTACTCCGGATGGATCCCCTAATATTACATCG
  GTCAGTCACAATTCAGTAAAGGTTAAGTTCAGCGGGTTTGAAGCCAGCCACGGACCTATCAAAGCCTATG
  CTGTCATCCTCACCACCGGGGAAGCTGCCCAACCTTCTGCAGATGTTTTGAAGTACACGTATGAGGATTT
  CAAAAGGGGAGCCTCGGATACTTATGTCACATACCTCATAAGAATAGAAGAGAAGGGACAGTCTCAGGGC
  TTGTCTGAAGTCTTGAACTATGAAATTGATGTGGGGAACCAATCCACTACCCTCGGCTACTACAACGGGA
  GGCTGGAGCCTCTGGGCTCCTACCGGGCTTGTGTTGCTGGCTTTACCAATATTACCTACAACCTTCAGAA
  TGACGGCCTCATCAATGGGGATGAGAGCTATGTGTCTTTCAGTCCATATTCAGAGGCCGTGTTCTTGCCC
  CAGGACCCAGGTGTCATCTGCGGAGCAGTGTTTGGATGTATCTTTGGTGCCCTGGCCATCACAGCTGTGG
  GAGGCTTCATCTTCTGGAGAAAGAAAAGGACAGATGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTAA
  ACCTAAAAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCCTACTTTAAGAAGCAGCAAGCTGACTCT
  AACTGTGGGTTTGCAGAGGAATATGAGGACCTGAAGCTGATTGGGATAAGTTTACCTAAATACACAGCTG
  AGATAGCCGAGAACAGAGGGAAGAACCGCTACAACAATGTTCTGCCCTATGATATTTCTCGAGTCAAACT
  TTCAGTCCAGACCCATTCGACAGATGACTACATCAATGCCAACTATATGCCTGGCTACCATTCCAAGAAA
  GATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAGATTTCTGGCGTATGGTTTGGGAGAAAA
  ACGTATATGCCATTGTTATGTTGACCAAATGCGTGGAGCAGGGAAGGACCAAATGTGAGGAGTACTGGCC
  TTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCGATGACATCAGAAGTCGTTCTTCCAGAATGG
  ACCATCAGAGATTTTGTGGTGAAAAATATGCAGAATAGCGAGAGCCATCCTCTGCGGCAGTTCCATTTCA
  CCTCCTGGCCTGACCACGGTGTTCCTGACACCACTGACCTGCTCATCAACTTTCGGTACCTGGTCCGGGA
  TTACATGAAGCAGATACCCCCCGAGTCACCAATTCTGGTGCATTGCAGTGCTGGGGTTGGAAGGACGGGC
  ACTTTCATCGCCATCGATCGCCTGATCTATCAGATAGAGAATGAGAACACCGTGGACGTGTATGGGATTG
  TCTATGACCTTCGGATGCACAGGCCTCTGATGGTGCAGACAGAGGACCAGTATGTTTTCCTCAATCAGTG
  TGTTTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGATCTCATCTATCAGAACACAACGGCAATG
  ACAATCTATGAAAACCTCGAGCCAGTGAGCATGTTTGGAAAGACTAATGGTTACATCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221777 representing NM_008982
  Red=Cloning site Green=Tags(s)
  
  MTRGGGRGSSRGRGSRELGATRGGWAPLAPPREAPASLRPRPLRARRARLRRVAAAAAAAMSPGKPGAGG
  AGTRRTGWRRRRRRRRLETETRAPGFGHTAGRVPGTFQGAQGMKPAARETRTPPRSPGLRWALLPLLLLL
  RQGQVLCAGAAPNPIFDIEAVVSPTSVLLTWKHNDSGASECRIENKMESNLTFPVKNQTSCNITGLSPGT
  SYTFSIISVTTNETLNKTITTEPWPVSDLHVTSVGVTQARLTWSNANGTASYRMLIEELTTHSSVNISGL
  KPGTNNSFAFPESNETQADFAVAEEVPDANGTKRIPVTNLSQLHKNSLVSVDPPSGQDPSLTEILLTDLK
  PDTQYNATIYSQAANGTEGQPRNKVFKTNSTQVSDVRAMNISASSMTLTWKSNYDGSRTSIVYKIHVAGG
  THSVNQTVNKTEAIILGLSSSTLYNITVHPFLGQTEGTPGFLQVYTSPDQVSDFRVTNVSTRAIGLAWRS
  NDSKSFEIFIKQDGGEKHRNASTGNQSYMVEDLKPGTSYHFEIIPRGPDGTEGLSSTVNGSTDPSAVTDI
  RVVNISTTEMQLEWQNTDDASGYTYHLVLESKSGSIIRTNSSQKWITVGSLTPGTLYNVTIFPEVDQIQG
  ISNSITQYTRPSSVSHIEVNTTTTTAAIRWKNEDAASASYAYSVLILKTGDGSNVTSNFTKDPSILIPEL
  IPGVSYTVKILTQVGDGTTSLVPGWNLFCTEPEPVTSFHCEVVPKEPALVLKWACPFGMYTGFELGVRSD
  SWDNMTRLENCTSDDDTECRTEVAYLNFSTSYNISIATLSCGKMALPAQNICTTGITDPPTPDGSPNITS
  VSHNSVKVKFSGFEASHGPIKAYAVILTTGEAAQPSADVLKYTYEDFKRGASDTYVTYLIRIEEKGQSQG
  LSEVLNYEIDVGNQSTTLGYYNGRLEPLGSYRACVAGFTNITYNLQNDGLINGDESYVSFSPYSEAVFLP
  QDPGVICGAVFGCIFGALAITAVGGFIFWRKKRTDAKNNEVSFSQIKPKKSKLIRVENFEAYFKKQQADS
  NCGFAEEYEDLKLIGISLPKYTAEIAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINANYMPGYHSKK
  DFIATQGPLPNTLKDFWRMVWEKNVYAIVMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSEVVLPEW
  TIRDFVVKNMQNSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQIPPESPILVHCSAGVGRTG
  TFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIIRAQKDSKVDLIYQNTTAM
  TIYENLEPVSMFGKTNGYIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008982
• ORF Size: 4050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008982.5, NP_033008.3
• RefSeq Size: 7634 bp
• RefSeq ORF: 4053 bp
• Locus ID: 19271
• UniProt ID: Q64455
• Cytogenetics: 2 50.19 cM
• MW: 149 kDa
• Gene Summary: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.[UniProtKB/Swiss-Prot Function]