Zfyve9 (NM_183300) Mouse Tagged ORF Clone

CAT#: MR221399

Zfyve9 (Myc-DDK-tagged) - Mouse zinc finger, FYVE domain containing 9 (Zfyve9)

Product Images

Circular map for MR221399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfyve9
• Synonyms: Madhip; NSP; SARA; SMADIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221399 representing NM_183300
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGAACAAAATGAAGGTTTTCTGTGCTTCCTGCTGCAGCCTAAAATGCAAACTGCTGTACATGG
  ACAGAAAGGAAGCCAGAGTGTGTGTAATCTGTCACTCAGTGCTGATGAATGCTCAAGCCTGGGAGAACAT
  GATGAGTGCCTCAAGCCAGAGCCCTAACCCTAACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTG
  CAGCAAGCTCAGGCCTCAGGAGCCCTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAA
  AGCACCCTGGAACAGAAGTGCCTCAGCCCAGAGAGCAGAGGAGAGTTTGGTTTGCTGATGGGATCTTACC
  TAATGGAGAAGTTGCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACTCTGGCTGTG
  TCACATGACCCAGTCAAGCCTGTAGCCACCAGTCCTCTTCCAACAGAGGCTGATACTTCTCTGTTCTCTG
  GGAGCATTACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAACCTTATTCCTGAAGATGGCCTTCCTCC
  CATCCTCATCTCCACTGGTGTGAAAGGAGATTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTCATG
  CAGCAACTGGAGGATGGTGGCCCTGACCCACTTGTCTTTGTTTTAAATGCAAATTTATTGTCAATGGTTA
  AAATTGTAAATTATGTGAACAGGAAGTGCTGGTGTTTTACAACCAAAGGGATGCATGCCGTGGGTCAATC
  TGAGATAGTTATTCTTCTGCAGTGTCTGCCAGATGAGAAATGTTTGCCTAAGGACATCTTTAATCACTTT
  GTACAGCTTTACCGGGACGCCCTGGCAGGGAATGTTGTGGGCAGCCTGGGACATTCTTTCTTCAGTCAAA
  GTTTCCTGGGCAGTAAAGAACATGGTGGGTTCTTGTATGTGACATCTACCTACCAGTCACTGCAAGACCT
  GGTGCTTCCAACCCCTCCTTACCTGTTTGGGATTCTCATCCAGAAATGGGAAACTCCTTGGGCCAAAGTG
  TTTCCCATACGACTCTTGTTAAGACTTGGAGCTGAATATCGACTTTACCCCTGTCCACTGTTCAGTGTCA
  GATTTCGGAAGCCATTATTTGGAGAGACTGGCCATACCATCATGAATCTTCTAGCAGACTTCCGAAATTA
  CCAGTATACTCTGCCAGTTGTCCAAGGTTTAGTGGTTGATATGGAAGTTCGAAAAACAAGCATCAAAATT
  CCCAGCAACAGATATAATGAGATGATGAAAGCCATGAACAAATCCAATGAACATGTACTGGCAGGAGGTG
  CCTGCTTCAATGAAAAGGCAGATTCTCATCTTGTGTGTGTACAGAACGATGATGGAAACTATCAAACCCA
  GGCCATCAGTATTCACAATCAGCCCAGGAAAGTGACTGGCGCCAGTTTCTTTGTGTTCAGTGGCGCACTG
  AAATCCTCTTCAGGATACCTTGCCAAGTCCAGTATTGTAGAAGATGGAGTCATGGTCCAGATCACTGCGG
  AGAACATGGACTCCTTGAGGCAGGCACTGCGGGAGATGAAGGACTTTACCATCACTTGTGGGAAGGCGGA
  TGCAGAGGACCCCCAGGAACAGATCCACATCCAGTGGGTGGACGATGACAAGACAGTTAACAAAGGTGTG
  GTGAGTCCTATAGATGGGAAGTCTATGGAATCTATAACAAACGTGAAAATATTCCATGGGTCAGAGTATA
  AAGCAAACGGAAAAGTCATCAGATGGACAGAGGTGTTTTTCCTGGAAAACGATGACCACCATAACTGCCT
  GAGTGACCCTGCAGATCACAGCAGACTGACTGAGCATGTCGCCAAGGCATTCTGCCTCGCCCTCTGCCCC
  CACCTGAAGCTTTTAAAAGAAGATGGAATGACAAAACTGGGACTCCGTGTGACGCTTGACTCAGATCAGG
  TTGGCTATCAAGCAGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAACGATCTGGACAGTGCCTT
  GGTGCCTGTCATTCATGGAGGAGCCTGCCAGCTCAGTGAAGGCCCTGTCGTCATGGAACTCATCTTTTAT
  ATTCTGGAAAACATCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221399 representing NM_183300
  Red=Cloning site Green=Tags(s)
  
  MNLNKMKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPAEYCSTIPPL
  QQAQASGALSSPPPTVMVPVGVLKHPGTEVPQPREQRRVWFADGILPNGEVADAAKLTMNGTSSAGTLAV
  SHDPVKPVATSPLPTEADTSLFSGSITQVGSPVGSAMNLIPEDGLPPILISTGVKGDYAVEEKPSQISVM
  QQLEDGGPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHF
  VQLYRDALAGNVVGSLGHSFFSQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKV
  FPIRLLLRLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKI
  PSNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASFFVFSGAL
  KSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEDPQEQIHIQWVDDDKTVNKGV
  VSPIDGKSMESITNVKIFHGSEYKANGKVIRWTEVFFLENDDHHNCLSDPADHSRLTEHVAKAFCLALCP
  HLKLLKEDGMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHGGACQLSEGPVVMELIFY
  ILENIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183300
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183300.4
• RefSeq Size: 2170 bp
• RefSeq ORF: 2121 bp
• Locus ID: 230597
• Cytogenetics: 4 C7
• MW: 77.5 kDa