Hps5 (NM_001167864) Mouse Tagged ORF Clone

CAT#: MR221111

Hps5 (Myc-DDK-tagged) - Mouse Hermansky-Pudlak syndrome 5 homolog (human) (Hps5), transcript variant 3

Product Images

Circular map for MR221111

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hps5
• Synonyms: AI646796; AL022647; C85120; haze; hz; maroon; mr; ru-2; ru2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTTGTGCCAGTGATACCAGAAGCCTACAGCCATGTTCTTGCAGAGTTTGAGTCCTTGGATCCGT
  TACTCACAGCCCTGAGGCTGGACTCCAGTCGTCTGAGGTGTACCAGCATAGCTGTGTCTCGGAAATGGTT
  GGCGTTAGGCAGCACAGGAGGAGGACTCAATCTCATTCAGAAAGATGGCTGGAAGCAAAGGCTGTTTCTC
  TCTCACCGGGAAGGGGCAATCTCTCAGATTGCCTGCTGCTCTCATGATGATGATTATGTTGCTGTAGCTA
  CCAGTCAAGGTCTTGTAGTTGTTTGGGAATTAAATCAAGAGCGTCGTGGGAAGCCAGAACGAATTCATGT
  GTCCTCAGAACACAAAGGCCGAAAAGTTACTGCCCTCTGTTGGGACACGGCTGTTCTGAGAGTCTTTGTC
  GGTGACCATGTGGGCAAAGTATCTGCCATCAAACTCAACACTTTGAAACAAGCAAAAGCAGCTGCTGCCT
  TTGTGATGTTTCCTGTGCAGACAGTACTAACAGTGGACTCGTGTGTCGTCCAGTTGGATTACTTGGATGG
  AAGATTACTTGTGTCTTCACTGACTCGATCCTTCTTGTGTGACACCGAGAGGGAAAAGTTTTGGAAAATC
  GGAAATAAGGAAAGACATGGAGAATATGGGGCTTGTTTCTTCCCCGGGCGGTGTGCTGGGGGCCAGCAGC
  CTGTGATCTACTGCGCCCGCCCAGGCTCCCGGATGTGGGAAGTGAACTTTGACGGGGAAGTGCTCAGTAC
  ACACCAGTTCAAGAAGCTCCTGTCAATGCCACCCCTCCCTGTGATCACTGCAAGATCAGAGCCTCAGTAT
  GATCACACAGTTGGATCCTCCCAGTCTTTGGCATTCCCCAAACTCTTGCATCTTAGTGAACACTGTGTGC
  TGACTTGGACAGAAAAGGGAATTTATATTTTCATTCCTCAGAATGTTCAAGTTCTTCTTTGGAGTGAAGT
  CAAGGAACGCCTGTTAAGGAGAGGCCTGTGGGACCTGGCTGCTCGCACATGCTGTCTCTTCCAAAATTCT
  ATTATTACCAGCAGAGCAAGAAAAACATTGACTGCAGATAAGTTGGAGCATTTGAAGTCTCAGCTGGACC
  TTACAGCCTGCAGTGAACTAATTTCTCAGCTGGACGACTTGATCTTAAGGTTCGAGCCTTTGGAGTCGGC
  GTGCAGCAGCAGAAGAAGCTCCATCTCATCGCATGAAAGTTTTAGCATCTTGGACTCTGGTATTTATCGT
  ATCATCAGTAGTAGAAGAGGCAGTCAGTCCGACGAAGATTCTTGTTCCCTTCACAGCCAGACCTTCTCAG
  AAGATGAAAGGCTTAAAGAATTTGCCTCACACCAGGAAGAGGAGCAGCCAGAGCAGGGCTGTGGTGCCAA
  TAGGAATGAAGAGAGTGCTTCCCACAGTCCAGTGATGTCTGAGGTAGATAAGAGTGAAGCTTTTCTGCCC
  TTCAGCATTGCACTGCCATTTCGTTCTCCATCTCCCCTCGTGTCTCTTCAGGCTGTTAAAGACAGTGTTT
  CTAGCTTTGTGCGTAAAACTACTGAGAAGATTGGCACCCTTCACGGAAGCCCCGAGCTGAAAGAGCCTTT
  TGAATCCAAGGATGCTGACCGAGCACATGAAGAGGAAGTGAGTGCAGTCACCTGCCCTCTGGAGGAGGAC
  ACTGAGGAGAAAGAAATTCATCAACCTCCTAAAGAAGACAGGCTTCAAGAACTCACAGCAGCGACAGCAG
  AAGCAATGACCAAGTTATTGGACCCTCTGGTTCTGTTTGAGCCCAAGGTGCTAAGAATGGTTTTACTTGA
  GTGGCTTTCACAGTTAGAGAAAACATTTGCCATGAAAGACTTCCCCGGCATTTCCAATACCAGCAGTCCA
  ACCGTGAAATCAAACTTGGGTGCACACCTCCTCGGTGAGACAGAAAAGAGAGTATTAGATGAAGAGAGTG
  GAGAAGGAAGAAGGGTCTCTTTAGTCACTGAAGAAGCTGGAGGTCAGATAACCTGTGACCCTGTAAGCAA
  CCTCAGTGAGCCCTCGGCTGACCGTTTTCGAGTATGCTCTCCATACGCCATCACAAACAGCCTTCAGAGG
  GACCTGGCGGAATTGACAACGTTGTGCTTGGAACTGAATGTGTTGACTTCAGCAATGGAAAGCGTAGGTG
  GGCATGTGGACCGGGCTTCACAGCAGCTCTCTCCAGAAATCCTGGCTTGTCGTTTCCTAAAGAAGTACTT
  TTTTCTTCTGGACTTGAAAAGAGCAAAGGAGAGTATCAAGCTCACTTACGACAGCCCTTGCGTTTGGGAC
  ACTTTTGTCGAAGGACTAAAAGAAATGGCAAGATCTAATCCTGCCTACACGGAGCTGGAAGAAGGAGACC
  TGCCCACGGGGTTACAGTTACTGGATGGCTCCGTCCCTTCTGATAGTCCCTTGTTGATTGCATTTGCCAC
  CCGGTTGTATGATAGATTTGGGGAGTCAGCCCTTCGAGCTTGCATCAAGTTTTATCCGTCCATTTCGCCC
  TCCGACATCGCACAGCTTTGCCGTCATCATCCTGCTCAGTTCTTGGCCTATCTAGACAGTCTGGTGAAGT
  CCAGGCCGGAAGACCAGTGGCCATCCTTCCTTGAGTTCCTTCTACAACCAGAGTCTTTAAGACTTGAATG
  GCTGCTTTTGGCAGTGTCCCATGATGCTCCACCAAGCACAAGTACAGTGGATGATGAAGGGCACCCCAGG
  CCCCATTCACACTTGCTTTCTTGGGGTTATAGTCAGCTGATTCTTCTTCTTATTAAACTTCCTGCAGACT
  TTACAACCAAAGAGAAAATGACTGACATCTGTAGGTCTTACGGTTTCTGGCCTGGATATCTTACCCTCTG
  TCTGGAGCTGGAGAGGAGGAGGGAGGCCTTCACCAACATTGTGTATCTGAATGACATAAGCCTGATGGAA
  GGGGACAATGGGTGGATCCCTGAGACCTTGGAGGAATGGAAGCTTCTCCTACATCTACTACAGACCAAGA
  GCACAAGGCCAGCTCCCCAGGAGTCACTAAACGGGAGCCTCAGTGATGGGCCAGCCCCTATCAATGTGGA
  GAATGTGGCCCTCCTGTTAGCTAAGGCCATGGGCCCAGATCGGGCCTGGTCACTGCTACAGGAGTGTGGT
  CTGGCTCTTGAGTTGTCAGAAAAGTTCACCAGAACCTGTGATATCCTGAGGATTGCTGAGAGAAGGCAGA
  GAGCGCTGATACAAGGCATGCTTGAGAAGTGTGACCGCTTCCTCTGGTCGCAGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTFVPVIPEAYSHVLAEFESLDPLLTALRLDSSRLRCTSIAVSRKWLALGSTGGGLNLIQKDGWKQRLFL
  SHREGAISQIACCSHDDDYVAVATSQGLVVVWELNQERRGKPERIHVSSEHKGRKVTALCWDTAVLRVFV
  GDHVGKVSAIKLNTLKQAKAAAAFVMFPVQTVLTVDSCVVQLDYLDGRLLVSSLTRSFLCDTEREKFWKI
  GNKERHGEYGACFFPGRCAGGQQPVIYCARPGSRMWEVNFDGEVLSTHQFKKLLSMPPLPVITARSEPQY
  DHTVGSSQSLAFPKLLHLSEHCVLTWTEKGIYIFIPQNVQVLLWSEVKERLLRRGLWDLAARTCCLFQNS
  IITSRARKTLTADKLEHLKSQLDLTACSELISQLDDLILRFEPLESACSSRRSSISSHESFSILDSGIYR
  IISSRRGSQSDEDSCSLHSQTFSEDERLKEFASHQEEEQPEQGCGANRNEESASHSPVMSEVDKSEAFLP
  FSIALPFRSPSPLVSLQAVKDSVSSFVRKTTEKIGTLHGSPELKEPFESKDADRAHEEEVSAVTCPLEED
  TEEKEIHQPPKEDRLQELTAATAEAMTKLLDPLVLFEPKVLRMVLLEWLSQLEKTFAMKDFPGISNTSSP
  TVKSNLGAHLLGETEKRVLDEESGEGRRVSLVTEEAGGQITCDPVSNLSEPSADRFRVCSPYAITNSLQR
  DLAELTTLCLELNVLTSAMESVGGHVDRASQQLSPEILACRFLKKYFFLLDLKRAKESIKLTYDSPCVWD
  TFVEGLKEMARSNPAYTELEEGDLPTGLQLLDGSVPSDSPLLIAFATRLYDRFGESALRACIKFYPSISP
  SDIAQLCRHHPAQFLAYLDSLVKSRPEDQWPSFLEFLLQPESLRLEWLLLAVSHDAPPSTSTVDDEGHPR
  PHSHLLSWGYSQLILLLIKLPADFTTKEKMTDICRSYGFWPGYLTLCLELERRREAFTNIVYLNDISLME
  GDNGWIPETLEEWKLLLHLLQTKSTRPAPQESLNGSLSDGPAPINVENVALLLAKAMGPDRAWSLLQECG
  LALELSEKFTRTCDILRIAERRQRALIQGMLEKCDRFLWSQQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167864
• ORF Size: 3279 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167864.1, NP_001161336.1
• RefSeq Size: 4729 bp
• RefSeq ORF: 3282 bp
• Locus ID: 246694
• UniProt ID: P59438
• Cytogenetics: 7 30.56 cM
• MW: 122.6 kDa
• Gene Summary: May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. Regulates intracellular vesicular trafficking in fibroblasts. May be involved in the regulation of general functions of integrins.[UniProtKB/Swiss-Prot Function]