Stk10 (NM_009288) Mouse Tagged ORF Clone

CAT#: MR220805

Stk10 (Myc-DDK-tagged) - Mouse serine/threonine kinase 10 (Stk10)

Product Images

Circular map for MR220805

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk10
• Synonyms: Gek1; L; Lok; mKIAA4026
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220805 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCGCCAATTTCCGCCGCATCTTGCGGTTATCCACCTTCGAGAAGAGAAAGTCCCGTGAATATG
  AGCACGTCCGCCGGGACCTGGACCCCAACGATGTATGGGAGATCGTGGGCGAGCTGGGAGACGGCGCCTT
  CGGCAAAGTCTACAAGGCCAAAAACAAGGAGACCGGTGCCCTGGCAGCAGCCAAAGTCATAGAGACCAAG
  AGCGAGGAGGAGCTGGAGGACTACATCGTGGAGATAGAGATCCTGGCCACCTGTGACCACCCGTATATCG
  TGAAGCTCCTGGGGGCCTATTACTATGATGGGAAGCTCTGGATCATGATTGAGTTCTGCCCTGGGGGAGC
  TGTGGATGCCATCATGCTGGAGCTGGATCGAGGCCTCACTGAGCCCCAGATTCAGGTCGTATGCCGCCAG
  ATGTTGGAGGCTCTCAACTTCCTGCACGGCAAGAGGATCATCCACCGAGACCTGAAAGCTGGCAACGTGC
  TTATGACCCTTGAGGGGGACATCAGGCTGGCTGATTTTGGTGTGTCTGCCAAGAATCTGAAAACTCTGCA
  AAAGCGAGACTCCTTCATAGGGACACCTTACTGGATGGCCCCTGAGGTGGTGCTGTGTGAAACCATGAAG
  GACGCCCCCTATGACTACAAGGCTGACATCTGGTCCTTGGGCATCACGCTGATCGAGATGGCGCAGATCG
  AGCCCCCACACCACGAGCTCAACCCCATGCGGGTCCTGCTCAAGATTGCCAAGTCGGACCCTCCTACCCT
  GCTTACACCCTCCAAGTGGTCTGTGGAGTTCCGAGACTTTCTGAAGATAGCCTTGGATAAGAACCCAGAA
  ACCCGGCCCAGTGCTGCACAGCTGCTGCAGCATCCCTTCGTCAGCAGAGTCACCAGCAATAAGGCTCTTC
  GGGAGCTGGTGGCCGAGGCAAAGGCGGAGGTAATGGAGGAGATTGAGGATGGCAGGGAGGACGGAGAAGA
  GGAGGATGCTGTGGATGCTGTTCCGCCCCTGGTCAACCACACTCAGGACTCTGCCAATGTAACTCAGCCA
  AGCCTCGACTCCAACAAGCTTCTCCAGGATTCTTCCACCCCCCTGCCTCCCAGCCAGCCTCAGGAGCCTG
  TGAACGGGCCCTGTAGCCAACCCTCTGGGGATGGACCCCTCCAAACCACCAGCCCTGCAGATGGGCTCTC
  CAAGAATGACAATGACTTAAAGGTGCCTGTTCCCCTCCGGAAGTCCCGGCCACTGTCCATGGATGCCAGA
  ATTCAGATGGATGAAGAGAAACAAATCCCTGACCAGGATGAGAACCCGAGTCCTGCAGCCAGCAAGTCCC
  AAAAGGCCAACCAGAGCCGGCCTAACAGCAGTGCCCTGGAGACTTTGGGTGGTGAGGCTCTGACCAACGG
  TGGCCTGGAACTCCCCAGCTCTGTCACTCCAAGCCATTCTAAGAGGGCCTCGGACTGTAGCAATCTGTCT
  ACCTCAGAGAGCATGGACTATGGCACCTCCCTGTCTGCTGACCTGTCACTGAACAAAGAAACGGGCTCAT
  TGTCTCTCAAGGGCTCAAAACTGCACAATAAGACCCTGAAACGGACCCGCCGGTTTGTGGTGGATGGTGT
  GGAGGTGAGCATCACCACCTCCAAGATCATCAGCGAGGATGAGAAGAAAGACGAGGAGATGAGATTTCTC
  AGGCGCCAGGAGCTCCGAGAGCTTCGGCTTCTGCAGAAAGAAGAACATCGGAACCAGACGCAGCTGAGCA
  GCAAGCACGAGCTGCAGCTGGAGCAGATGCACAAACGTTTTGAACAAGAAATCAACGCCAAGAAGAAATT
  CTATGACGTGGAGCTAGAAAACCTGGAGCGGCAGCAGAAGCAGCAGGTAGAGAAGATGGAGCAGGACCAC
  AGCGTGCGCCGCAAAGAGGAGGCCAAGCGGATCCGCCTGGAGCAGGATCGAGACTACGCCAAGTTCCAAG
  AGCAACTCAAGCAAATGAAGAAGGAGGTGAAGAGTGAGGTTGAGAAACTGCCCCGGCAGCAGCGGAAGGA
  GAGCATGAAGCAGAAGATGGAGGAACACTCTCAGAAGAAACAGCGGCTGGACCGGGACTTTGTAGCCAAG
  CAGAAGGAGGACCTGGAGCTGGCCATGAGGAAGCTCACCACGGAAAACAGGCGCGAGATCTGTGACAAGG
  AGCGAGATTGCCTTAGCAAGAAGCAGGAGCTCCTCCGAGACCGTGAGGCGGCCCTGTGGGAGATGGAGGA
  GCACCAGTTGCAGGAGCGACATCAGCTGGTGAAGCAGCAACTGAAGGACCAGTACTTCCTGCAGCGGCAT
  GACCTGCTGCGTAAGCATGAGAAGGAGCGGGAACAGATGCAGCGCTACAACCAGCGCATGATGGAGCAGC
  TGAAGGTCAGACAGCAACAGGAGAAGGCGCGGCTACCCAAGATCCAGAGGAGCGACGGCAAGACGCGCAT
  GGCCATGTACAAGAAGAGCCTGCACATCAACGGTGCGGGCAGTGCCTCTGAGCAGCGCGAGAAGATCAAG
  CAGTTCTCCCAGCAGGAGGAGAAGAGGCAGAAGGCGGAGAGGCTGCAGCAGCAGCAGAAACACGAGAACC
  AGATGCGAGACATGGTGGCGCAGTGTGAAAGCAACATGAGCGAGCTACAGCAGCTGCAGAATGAAAAGTG
  TCATCTGTTGGTAGAGCATGAAACCCAGAAGCTGAAGGCCCTGGATGAGAGCCACAACCAGAGCCTGAAG
  GAATGGCGAGACAAGCTTCGGCCACGCAAAAAGGCCCTGGAAGAGGATTTGAACCAGAAGAAGCGGGAAC
  AGGAAATGTTCTTCAAATTAAGCGAGGAGGCAGAGCCCAGACCCACCACACCCAGCAAAGCCAGCAACTT
  CTTCCCCTACAGCTCTGGGGATGCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220805 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNDVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETK
  SEEELEDYIVEIEILATCDHPYIVKLLGAYYYDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQ
  MLEALNFLHGKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVLCETMK
  DAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPE
  TRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQP
  SLDSNKLLQDSSTPLPPSQPQEPVNGPCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDAR
  IQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLS
  TSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFL
  RRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDH
  SVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAK
  QKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRH
  DLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIK
  QFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLK
  EWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009288
• ORF Size: 2901 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009288.2, NP_033314.2
• RefSeq Size: 5082 bp
• RefSeq ORF: 2901 bp
• Locus ID: 20868
• UniProt ID: O55098
• Cytogenetics: 11 A4
• MW: 111.9 kDa
• Gene Summary: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008]