Nsun2 (NM_145354) Mouse Tagged ORF Clone

CAT#: MR220146

Nsun2 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2)

Product Images

Circular map for MR220146

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nsun2
• Synonyms: D13Wsu123e; Misu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220146 representing NM_145354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGCGGGCGCGGGGCCGGCGGTTCCAGCAGCCGCCGCAGCCTGAGGGCGAGGAAGACGCCAGCG
  ACGGCGGCAGAAAGCGAGGCCAGGCGGGCTGGGAAGGTGGCTATCCCGAGATCGTAAAGGAGAACAAGCT
  CTTCGAGCACTACTATCAGGAACTCAAGATCGTGCCAGAGGGAGAATGGGACCAATTCATGGAGTCACTC
  CGAGAACCTCTCCCAGCCACACTGAGAATCACTGGGTACAAAAGCCATGCCAAAGAGATTCTCCATTGCT
  TGAAGAACAAGTACTTTAAGGAGTTGGAGGACCTGGAAGTAGATGGACAGAAAGTTGAAGTTCCACAACC
  ACTAAGCTGGTACCCTGAAGAACTTGCCTGGCATACAAACTTAAGTCGGAAAATCTTGAGGAAGTCCCCG
  TTGTTGGCAAAGTTCCATCAGTTCCTGGTCAGCGAGACTGAGTCTGGAAACATCAGCCGCCAGGAGGCTG
  TCAGCATGATCCCCCCACTGCTGCTCAACGTGGAGCCACACCATAAGATCTTAGACATGTGTGCAGCCCC
  TGGATCCAAGACCACACAGTTAATTGAAATGTTGCATGCAGACATGAGTGTGCCCTTTCCAGAGGGATTT
  GTAATCGCAAATGACGTGGACAACAAGCGCTGCTATCTGCTCGTCCATCAGGCCAAAAGGTTGAGCAGTC
  CCTGCATCATGGTGGTAAACCATGACGCATCCAGCATACCTAGACTTACAGTAGATGTGGACGGAAGGAA
  AGAGATTCTCTTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGCGATGGCACAATGAGAAAAAAC
  ATTGATGTCTGGAAGAAATGGACAACCTTAAACAGCTTGCAGCTCCATGGCCTGCAGCTTCGGATTGCAA
  CTCGAGGTGCTGAGCAGCTGGCGGAAGGTGGCAGGATGGTGTATTCCACGTGTTCCTTGAACCCCGTGGA
  GGATGAAGCAGTGATCGCAGCTCTGCTAGAGAAGAGTGAAGGAGCTCTTGAGCTTGCTGATGTGTCTGCT
  GAGTTGCCAGGACTGAAGTGGATGCCTGGAGTCTCACAGTGGAAGGTCATGACTAGAGACGGGCAGTGGT
  TTGCAGACTGGCATGAGGTTCCCCAGGGCAGGCATACACAAATCCGACCTACCATGTTCCCACCAACGGA
  CCTGGAGAAGCTACAGGCAATGCATCTAGAGCGATGCCTTCGAATCCTGCCCCATCATCAGAATACTGGA
  GGGTTCTTTGTGGCAGTATTGGTCAAGAAAGCACCAATGCCGTGGAACAAACGTCAGCCCAAGGTCCAGA
  ATAAATCTGCAGAAGCCAGAGAACCCAGGGTATCCAGCCATGTGGCTGCCACAGAGGGAAATCCCAGTGA
  CCAGTCTGAGCTGGAAAGTCAGATGATAACTGGAGCTGGTGACTCAGAAACAGCTCACAACACTGAGAAC
  ACAGAGAGCAATGAGAAGAAAGATGGCGTGTGTGGCCCTCCTCCATCAAAGAAAATGAAGTTGTTTGGAT
  TTAAAGAAGATCCATTTGTATTCATTCCTGAAGATGATCCTTTATTTCCACCTATTGAGAAGTTTTATGC
  CTTGGATCCTTCATTCCCGAGGATGAATCTGTTAACCCGAACCACAGAAGGAAAGAAGCGGCAGCTTTAT
  ATGGTCTCCAAGGAGCTGAGGAATGTACTGCTGAACAACAGCGAGAAGATGAAGGTCATTAACACTGGGA
  TAAAAGTCTGGTGTCGAAATAACAGTGGTGAAGAATTCGATTGTGCATTCCGTTTGGCACAGGAGGGAAT
  ATATACATTGTATCCATTTATCAATTCAAGAATCATCACTGTATCAATGGAAGACGTGAAGACACTGTTG
  ACCCAGGAGAACCCATTCTTTAGAAAACTGAGCAGTGAGGCCTACAGTCAAGTCAAGGACCTTGCAAAGG
  GAAGTGTTGTGCTGAAGTATGAGCCAGATTCTGCGAATCCAGACACCCTCCAGTGCCCCATCGTGCTGTG
  TGGGTGGCGGGGAAAGGCCTCTATTCGAACTTTTGTGCCCAAAAATGAGCGGCTTCATTATCTCAGGATG
  ATGGGTCTGGAGGTACTGGGAGAAAAGAAGAAAGAGGGAGTCATTCTTACCAATGAGAATGCTGCCAGCC
  CAGAGCAGCCTGGAGATGAGGATGCCAAGCAGACAGCACAAGACCCCTGCGTCCCAGACTCCGTCCCTGG
  CTGTGATGCAGCTGCAGCTGAGCCATCCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220146 representing NM_145354
  Red=Cloning site Green=Tags(s)
  
  MGRRARGRRFQQPPQPEGEEDASDGGRKRGQAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMESL
  REPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSP
  LLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGF
  VIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTVDVDGRKEILFYDRILCDVPCSGDGTMRKN
  IDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSA
  ELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRPTMFPPTDLEKLQAMHLERCLRILPHHQNTG
  GFFVAVLVKKAPMPWNKRQPKVQNKSAEAREPRVSSHVAATEGNPSDQSELESQMITGAGDSETAHNTEN
  TESNEKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLY
  MVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLL
  TQENPFFRKLSSEAYSQVKDLAKGSVVLKYEPDSANPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRM
  MGLEVLGEKKKEGVILTNENAASPEQPGDEDAKQTAQDPCVPDSVPGCDAAAAEPSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145354
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145354.5, NP_663329.3
• RefSeq Size: 4279 bp
• RefSeq ORF: 2274 bp
• Locus ID: 28114
• UniProt ID: Q1HFZ0
• Cytogenetics: 13 35.55 cM
• MW: 85.9 kDa
• Gene Summary: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity). May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.[UniProtKB/Swiss-Prot Function]