Map3k9 (NM_177395) Mouse Tagged ORF Clone
CAT#: MR219476
- TrueORF®
Map3k9 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_177395" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Map3k9 |
Synonyms | E130314H24Rik; Mlk1; Prke1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219476 representing NM_177395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCTCCAGATCGCTTCTCGGCTGCCTGGCGAGTGCCACTGCCGCCCCGCCGGGGGACGATGCCA CGGGCGCTGGGGCCGAAGAGGAGGAGGACGAGGAGGAGGCGGCGGCCGAGCTGGGATCTCACGCCGCGCT GCCCTACTGGACGGCTGTGTTCGAGTACGAGGCGGCGGGCGAGGACGAGCTGACCCTGCGGCTGGGCGAT GTGGTAGAGGTGCTGTCCAAGGACTCGCAGGTGTCCGGCGATGAGGGCTGGTGGACCGGACAGCTGAACC AGCGGGTGGGCATCTTCCCCAGCAACTACGTGACCCCGCGTAGCGCCTTCTCCAGCCGCTGCCAGCCGGG CGCCGAGGACCCCAGCTGCTACCCGCCCATTCAGCTGTTAGAGATTGATTTTGCGGAGCTAACCCTGGAG GAGATCATCGGCATTGGGGGCTTTGGGAAAGTTTATCGTGCTTTCTGGGCGGGCGATGAGGTGGCCGTGA AGGCAGCTCGTCACGACCCTGATGAGGACATCAGCCAGACCATAGAGAACGTTCGCCAAGAGGCCAAGCT CTTTGCCATGCTGAAGCACCCGAACATCATTGCGCTCAGAGGGGTGTGCCTGAAGGAACCCAACCTCTGC TTGGTCATGGAGTTTGCTCGTGGAGGGCCTCTGAACAGAGTATTGTCTGGGAAGAGGATTCCCCCGGACA TCCTGGTGAACTGGGCCGTGCAGATCGCCAGAGGGATGAACTATCTACATGATGAGGCGATCGTACCCAT CATCCACCGAGACCTTAAGTCCAGCAACATATTGATCCTGCAGAAAGTGGAGAATGGAGACCTGAGTAAC AAGATTCTGAAGATCACCGACTTTGGGCTGGCGCGGGAATGGCACCGGACCACCAAGATGAGTGCGGCGG GAACATACGCTTGGATGGCACCTGAAGTCATCCGTGCTTCCATGTTTTCCAAAGGCAGCGATGTGTGGAG CTACGGTGTACTGCTTTGGGAGCTGTTGACTGGCGAGGTGCCCTTCCGGGGCATTGATGGCTTAGCAGTG GCTTACGGTGTGGCCATGAACAAACTCGCCCTTCCTATCCCCTCTACATGTCCAGAGCCTTTTGCCAAAC TCATGGAAGACTGCTGGAATCCCGACCCCCACTCGCGCCCATCTTTCACGAGTATCCTGGACCAGCTAAC GACTATAGAGGAGTCCGGTTTCTTTGAGATGCCCAAGGACTCCTTCCACTGCCTGCAGGACGACTGGAAA CATGAGATTCAGGAGATGTTTGACCAACTCAGGGCCAAAGAAAAGGAGCTCCGAACCTGGGAAGAGGAGC TGACCCGGGCTGCGCTCCAGCAGAAGAACCAGGAGGAGCTGCTCCGGCGTAGGGAGCAGGAGCTGGCGGA GCGGGAGATCGACATCCTGGAGCGAGAGCTCAATATCATCATCCACCAGCTGTGCCAGGAAAAGCCCCGG GTGAAGAAACGCAAGGGCAAGTTCAGGAAGAGCCGGCTGAAGCTCAAGGACGGCAACCGCATCAGCCTCC CCTCCGATTTCCAGCACAAGTTCACGGTGCAGGCCTCCCCGACCATGGATAAAAGGAAGAGTCTGATCAG CAACCGGTCGAGTCCTCCTGCAAGCCCCACCATCATCCCTCGCCTTCGAGCCATCCAGTTGACACCTGGT GAAAGCAGTAAAACCTGGGGCCGGAGCTCAGTTGTTCCAAAAGAGGAAGGGGAGGAGGAGGAGAAGAGGG CCCCAAAGAAGAAGGGCCGGACATGGGGACCAGGAACACTTGGGCAGAAAGAGCTCACATCAGGAGATGA AGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219476 representing NM_177395
Red=Cloning site Green=Tags(s) MESSRSLLGCLASATAAPPGDDATGAGAEEEEDEEEAAAELGSHAALPYWTAVFEYEAAGEDELTLRLGD VVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGAEDPSCYPPIQLLEIDFAELTLE EIIGIGGFGKVYRAFWAGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLC LVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSN KILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAV AYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTSILDQLTTIEESGFFEMPKDSFHCLQDDWK HEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPR VKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLISNRSSPPASPTIIPRLRAIQLTPG ESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELTSGDEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177395 |
ORF Size | 1824 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177395.5, NP_796369.2 |
RefSeq Size | 3396 bp |
RefSeq ORF | 1827 bp |
Locus ID | 338372 |
UniProt ID | Q3U1V8 |
Cytogenetics | 12 D1 |
MW | 68.6 kDa |
Gene Summary | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219673 | Map3k9 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9), transcript variant 2, (10ug) |
USD 624.00 |
|
MG219476 | Map3k9 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9) transcript variant 2, (10ug) |
USD 822.00 |
|
MR219476L3 | Lenti ORF clone of Map3k9 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9), transcript variant 2 |
USD 922.00 |
|
MR219476L4 | Lenti ORF clone of Map3k9 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9), transcript variant 2 |
USD 922.00 |
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