Dnajb6 (NM_001037940) Mouse Tagged ORF Clone
CAT#: MR219416
- TrueORF®
Dnajb6 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 6 (Dnajb6), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001037940" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dnajb6 |
Synonyms | HSJ-2; mDj4; Mrj |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219416 representing NM_001037940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGATTACTATGAAGTGCTGGGCGTGCAGAGACATGCCTCACCTGAGGACATTAAAAAGGCGTATC GAAAACAGGCACTTAAATGGCACCCGGACAAAAATCCTGAAAATAAAGAAGAAGCAGAGCGGAAGTTCAA ACAAGTAGCTGAGGCATATGAAGTGTTATCGGATGCTAAAAAGCGGGACATCTACGACAAATATGGCAAA GAAGGATTAAATGGTGGTGGAGGAGGAGGTGGAATTCATTTTGACAGTCCATTTGAGTTTGGCTTCACAT TCCGGAACCCAGATGATGTCTTCAGGGAATTTTTTGGTGGAAGGGACCCATTTTCATTTGACTTCTTTGA AGACCCATTTGATGACTTTTTTGGAAACCGAAGGGGTCCCCGAGGAAATAGAAGCCGAGGTGCCGGCTCA TTTTTCTCTACCTTCAGTGGATTTCCTTCTTTTGGAAGTGGATTTCCTGCTTTTGATACAGGCTTCACTC CATTTGGGTCACTAGGTCATGGGGGTCTCACTTCATTTTCTTCAACGTCATTTGGCGGCAGTGGAATGGG CAACTTCAAATCAATATCAACTTCAACTAAGATAGTTAATGGCAAAAAAATCACGACAAAGAGGATTGTG GAGAACGGTCAAGAAAGAGTAGAAGTTGAAGAAGATGGGCAGTTAAAGTCCTTGACAATAAATGGTGTGG CCGACGAGAACGCCCTTGCAGAAGAATGCCAGCGGAGAGGCCAACCCACACCAGCTCTGGCCCCTGGCCC GGCCCCAGCACCAGTCCGGGTCCCCTCCCAGGCCCGGCCCCTGGCCCCAACTCCAGCCCCAACTCCAGCC CCAACTCCAGCCCCAGCTCCAGCCCAGACTCCGGCCCCTTCTGTCAGCACCCGCCCACAGAAGCCACCCA GGCCTGCCCCAACGGCCAAACTTGGGTCTAAAAGCAACTGGGAAGATGACGAGCAGGACAGGCAGCGGGT TCCTGGCAACTGGGATGCCCCTATGACCTCAGCAGGGCTCAAAGAAGGTGGCAAGAGGAAGAAGCAGAAG CAGAAAGAGGACTTGAAGAAGAAGAAGTCGACCAAAGGGAACCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219416 representing NM_001037940
Red=Cloning site Green=Tags(s) MVDYYEVLGVQRHASPEDIKKAYRKQALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGK EGLNGGGGGGGIHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFDDFFGNRRGPRGNRSRGAGS FFSTFSGFPSFGSGFPAFDTGFTPFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKIVNGKKITTKRIV ENGQERVEVEEDGQLKSLTINGVADENALAEECQRRGQPTPALAPGPAPAPVRVPSQARPLAPTPAPTPA PTPAPAPAQTPAPSVSTRPQKPPRPAPTAKLGSKSNWEDDEQDRQRVPGNWDAPMTSAGLKEGGKRKKQK QKEDLKKKKSTKGNH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001037940 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037940.4, NP_001033029.1 |
RefSeq Size | 2708 bp |
RefSeq ORF | 1098 bp |
Locus ID | 23950 |
UniProt ID | O54946 |
Cytogenetics | 5 B1 |
MW | 40.3 kDa |
Gene Summary | Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG219416 | Dnajb6 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily B member 6 (Dnajb6) transcript variant 1, (10ug) |
USD 703.00 |
|
MR219416L3 | Lenti ORF clone of Dnajb6 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 6 (Dnajb6), transcript variant 1 |
USD 803.00 |
|
MR219416L4 | Lenti ORF clone of Dnajb6 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 6 (Dnajb6), transcript variant 1 |
USD 803.00 |
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