Cbs (NM_144855) Mouse Tagged ORF Clone
CAT#: MR219119
- TrueORF®
Cbs (Myc-DDK-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_144855" in other vectors (6)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cbs |
Synonyms | AI047524; AI303044; HIP4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219119 representing NM_144855
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCAGGGACATCCCAGTGTGAAGATGGCTCTGCTGGGGGCTTCCAGCACTTGGACATGCACTCAG AAAAGAGACAACTGGAGAAGGGCCCCTCAGGGGACAAGGATCGAGTCTGGATCCGGCCTGATACCCCAAG CAGATGTACCTGGCAGCTGGGCAGGGCCATGGCGGACTCCCCACATTATCACACAGTGCTGACCAAATCC CCCAAAATTTTACCAGATATTCTGAGGAAAATTGGGAACACCCCTATGGTCAGAATCAACAAGATCTCAA AGAATGCCGGTCTCAAGTGTGAGCTCTTGGCCAAGTGTGAGTTCTTCAATGCGGGTGGGAGTGTGAAGGA CCGCATCAGCCTTCGGATGATCGAAGATGCTGAGCGAGCTGGAAACTTGAAGCCTGGAGACACTATCATT GAGCCAACTTCTGGCAACACAGGGATCGGGCTGGCTCTGGCTGCTGCAGTGAAGGGCTATCGCTGCATTA TCGTGATGCCGGAGAAGATGAGTATGGAGAAGGTGGATGTGCTGCGGGCTCTGGGAGCCGAGATTGTGAG GACGCCCACCAATGCCAGATTTGATTCCCCCGAGTCCCACGTGGGAGTGGCATGGCGACTGAAGAACGAA ATCCCTAATTCTCACATTCTGGACCAGTACCGCAATGCCAGCAACCCTTTGGCACACTACGATGACACCG CCGAGGAGATCCTGCAGCAGTGTGACGGGAAGCTGGATATGCTGGTGGCTTCAGCAGGCACGGGTGGCAC CATCACAGGGATCGCCAGAAAGCTGAAGGAGAAGTGCCCTGGCTGTAAAATCATCGGTGTCGATCCTGAA GGCTCCATCCTTGCGGAGCCCGAGGAGCTGAACCAGACGGAGCAAACAGCCTATGAGGTGGAAGGGATTG GCTACGACTTCATCCCGACAGTCCTGGACAGGGCGGTGGTGGATAAGTGGTTCAAGAGCAACGATGAAGA TTCCTTCGCCTTTGCCCGCATGCTCATCGCACAGGAAGGACTGCTATGTGGTGGAAGCTCTGGCAGCGCC ATGGCTGTGGCTGTGAAGGCTGCCCGGGAGCTGCAGGAAGGGCAGCGCTGTGTGGTCATCCTGCCTGACT CTGTGCGGAACTACATGTCCAAGTTCCTGAGTGACAAATGGATGCTGCAGAAAGGTTTCATGAAAGAGGA GCTCTCAGTGAAGAGGCCCTGGTGGTGGCGTCTGCGTGTTCAAGAGCTGAGCCTGTCGGCCCCGCTGACC GTGTTGCCCACGGTCACCTGTGAGGACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACCTG TGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACCCTCGGGAACATGCTGTCATCCCTGCTGGCTGG AAAGGTGCGGCCATCAGACGAAGTCTGCAAAGTCCTCTACAAGCAGTTCAAACCGATCCACCTGACCGAC ACGCTGGGCACACTCTCTCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTCCACGAGCAGATCCAAT CACGAGACCAGGCCTGGTCAGGAGTGGTGGGGGGGCCCACAGACTGCAGCAATGGCATGTCCAGCAAGCA GCAGATGGTGTTTGGGGTTGTCACTGCCATTGACCTGCTAAACTTCGTGGCAGCCCGTGAGCAGACCCAG ACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219119 representing NM_144855
Red=Cloning site Green=Tags(s) MPSGTSQCEDGSAGGFQHLDMHSEKRQLEKGPSGDKDRVWIRPDTPSRCTWQLGRAMADSPHYHTVLTKS PKILPDILRKIGNTPMVRINKISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGNLKPGDTII EPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE IPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPE GSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDKWFKSNDEDSFAFARMLIAQEGLLCGGSSGSA MAVAVKAARELQEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWRLRVQELSLSAPLT VLPTVTCEDTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTD TLGTLSHILEMDHFALVVHEQIQSRDQAWSGVVGGPTDCSNGMSSKQQMVFGVVTAIDLLNFVAAREQTQ T TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144855 |
ORF Size | 1683 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144855.3, NP_659104.1 |
RefSeq Size | 2503 bp |
RefSeq ORF | 1686 bp |
Locus ID | 12411 |
UniProt ID | Q91WT9 |
Cytogenetics | 17 16.93 cM |
MW | 62 kDa |
Gene Summary | Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218938 | Cbs (untagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1, (10ug) |
USD 575.00 |
|
MG219119 | Cbs (tGFP-tagged) - Mouse cystathionine beta-synthase (Cbs) transcript variant 1, (10ug) |
USD 983.00 |
|
MR219119L1 | Lenti ORF clone of Cbs (Myc-DDK-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1 |
USD 1,083.00 |
|
MR219119L2 | Lenti ORF clone of Cbs (mGFP-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1 |
USD 1,083.00 |
|
MR219119L3 | Lenti ORF clone of Cbs (Myc-DDK-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1 |
USD 1,083.00 |
|
MR219119L4 | Lenti ORF clone of Cbs (mGFP-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 1 |
USD 1,083.00 |
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