Nt5c3b (NM_026561) Mouse Tagged ORF Clone
CAT#: MR218685
- TrueORF®
Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (Nt5c3l), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_026561" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Nt5c3b |
Synonyms | 2610037D24Rik; AI841843; C330027I04Rik; Nt5c3l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218685 representing NM_026561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGAGGTAAGCAGCCTGATGAAGGCCACGGTCCTGATGCGGCAGCCCGGACGGGTGCAGGAAA TTGTGGGCGCCCTTCGCAGGGGCGGAGGAGACCGCCTGCAGGTGATTTCCGATTTTGACATGACCCTGAG CAGATTTGCTTACAATGGACAGCGATGCCCTTCTTCCCACAATATTCTGGATAACAGCAAAATTATCAGT GAGGATTGTCGCAAAGAGCTCACCGAGCTCTTTCACCACTATTATCCCATTGAGATCGACCCACACCGGA CCATCAAAGAGAAGCTGCCTCACATGGTGCAGTGGTGGAGCAAAGCCCACAGTCTCCTGTGCCAGCAGAG GATCCAGAAAGTCCAGATCGCTCAGGTGGTTGGAGAGTCCACTGCGATGCTCAGGGAGGGCTATAAGACG TTCTTCGACACACTCTACCAGAACAACATCCCCCTTTTCATCTTCTCAGCGGGCATTGGTGACATCCTGG AAGAAATTATCCGACAGATGAAAGTGTTCCACCCCAACATCCACATTGTGTCCAACTACATGGACTTCAG TGAGGATGGATTCCTGAAAGGATTCAAGGGGCAGCTCATTCACACCTACAACAAGAACAGCTCCGTGTGT GAGAACTCCAGCTACTTCCAGCAACTTCAGAACAAGACCAACATCATCCTGCTGGGAGACTCCATCGGGG ACCTCACCATGGCTGATGGGGTCCCCGGGGTGCAGAACATTCTCAAGATCGGCTTCCTGAATGACAAGCT CCCCGAGGCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218685 representing NM_026561
Red=Cloning site Green=Tags(s) MAEEVSSLMKATVLMRQPGRVQEIVGALRRGGGDRLQVISDFDMTLSRFAYNGQRCPSSHNILDNSKIIS EDCRKELTELFHHYYPIEIDPHRTIKEKLPHMVQWWSKAHSLLCQQRIQKVQIAQVVGESTAMLREGYKT FFDTLYQNNIPLFIFSAGIGDILEEIIRQMKVFHPNIHIVSNYMDFSEDGFLKGFKGQLIHTYNKNSSVC ENSSYFQQLQNKTNIILLGDSIGDLTMADGVPGVQNILKIGFLNDKLPEAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026561 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026561.5 |
RefSeq Size | 1427 bp |
RefSeq ORF | 786 bp |
Locus ID | 68106 |
UniProt ID | Q3UFY7 |
Cytogenetics | 11 D |
MW | 30.2 kDa |
Gene Summary | Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210690 | Nt5c3b (untagged) - Mouse 5'-nucleotidase, cytosolic III-like (Nt5c3l), transcript variant 2, (10ug) |
USD 330.00 |
|
MG218685 | Nt5c3l (tGFP-tagged) - Mouse 5'-nucleotidase cytosolic III-like (Nt5c3l) transcript variant 2, (10ug) |
USD 530.00 |
|
MR218685L3 | Lenti ORF clone of Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (Nt5c3l), transcript variant 2 |
USD 630.00 |
|
MR218685L4 | Lenti ORF clone of Nt5c3l (mGFP-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (Nt5c3l), transcript variant 2 |
USD 630.00 |
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