Rnf14 (NM_001164621) Mouse Tagged ORF Clone
CAT#: MR218588
- TrueORF®
Rnf14 (Myc-DDK-tagged) - Mouse ring finger protein 14 (Rnf14), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001164621" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rnf14 |
Synonyms | 2310075C09Rik; 2610005D23Rik; AA986456; AU041447; D7Bwg0165e; D18Ertd188e; Triad2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218588 representing NM_001164621
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCAGAAGACCTGGAAGCCCAGGAGGATGAACTACTGGCCCTGGCGAGTATTTATGATGCGGATG AATTTAGAAAAGCTGAGTCTGTCCAAGGAGGAGAAACCAGGATCTATTTGGATCTGCCACAGAATTTTAA GATATTCGTGAGCGGCAATTCAAATGAGTCTCTCCAGAATAGTGGCTTTGAATACACCATTTGCTTTCTG CCACCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCCTCCCCACCTTCATTCACACTTA GTGGCAAATGGCTGTCACCAACTCAGCTCTCTGCTCTCTGCAAGCACCTAGACAACCTGTGGGAAGAACA CCGAGGCAGAGTGGTGCTGTTTGCCTGGATGCAGTTTCTTAAGGAGGAGACCCTCACCTACCTGAATATT GTCTCTCCTTTTGAGCTCAAGATGGGGTCTCAGAAAAAAGTTCAGAGAAGGGCAACAGCCCAGGCCTCTT CCAGCACAGAGCTTGGTGTGGGAGGAGCTGCTGCGGCGGATGTAGACCAAGAAGAAACTGTGGATGAGAG AGCTGTACAGGATGTAGAGTCATTGTCAAGTCTGATCCAGGAGATCTTGGACTTCAATCAAGCTCGGCAG ACGAAGTGCTTCAATAGTAAACTGTTCTTGTGCAGCATCTGTTTCTGTGAGAAGCTGGGTAGTGATTGCA TGTACTTCTTGGAGTGCAAGCATGTATACTGCAAAGCCTGTCTGAAGGATTACTTTGAAATCCAGATCAA AGATGGTCAAGTTAAATGCCTCAACTGTCCAGAGCCACAGTGCCCTTCAGTGGCGACTCCTGGTCAGGTC AAAGAGCTGGTAGAAGCAGACTTGTTTGCTCGCTATGACCGTCTCCTCCTGCAGTCTACCTTGGATCTAA TGGCAGATGTGGTGTATTGTCCCCGTCCATGCTGCCAGCTCCCTGTGATGCAAGAGCCTGGTGGTACCAT GGCTATCTGCTCCAGCTGCAATTTTGCCTTCTGTACCTTATGCAGACTGACCTATCACGGGCTTTCTCCA TGTAAGGTAACTGCAGAGAAATTAATAGACTTACGAAATGAATACCTACAAGCAGATGAAGCTACTAAGA GGTTCTTGGAACAGAGGTATGGCAAGAGGGTGATTCAGAAGGCCCTGGAGGAGATGGAAAGTAAAGACTG GCTGGAGAAGAACTCCAAGAGCTGCCCCTGCTGTGGCACACCCATACAGAAATTAGACGGCTGCAACAAG ATGACTTGCACTGGCTGTATGCAGTATTTCTGCTGGATTTGCATGGGTTCTCTCTCTCGAGCAAATCCTT ACAGACATTTCACTGATTCTGAGTCTCCATGCTTTAACAGGTTGTTCCATGCCGTGGATATTAATGGTGA TATGTGGGAAGATGAAATTGAGGAGGACGACGATGATGAAGACGACGACGACGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218588 representing NM_001164621
Red=Cloning site Green=Tags(s) MSAEDLEAQEDELLALASIYDADEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNESLQNSGFEYTICFL PPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGRVVLFAWMQFLKEETLTYLNI VSPFELKMGSQKKVQRRATAQASSSTELGVGGAAAADVDQEETVDERAVQDVESLSSLIQEILDFNQARQ TKCFNSKLFLCSICFCEKLGSDCMYFLECKHVYCKACLKDYFEIQIKDGQVKCLNCPEPQCPSVATPGQV KELVEADLFARYDRLLLQSTLDLMADVVYCPRPCCQLPVMQEPGGTMAICSSCNFAFCTLCRLTYHGLSP CKVTAEKLIDLRNEYLQADEATKRFLEQRYGKRVIQKALEEMESKDWLEKNSKSCPCCGTPIQKLDGCNK MTCTGCMQYFCWICMGSLSRANPYRHFTDSESPCFNRLFHAVDINGDMWEDEIEEDDDDEDDDDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164621 |
ORF Size | 1455 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164621.1, NP_001158093.1 |
RefSeq Size | 3369 bp |
RefSeq ORF | 1458 bp |
Locus ID | 56736 |
UniProt ID | Q9JI90 |
Cytogenetics | 18 20.2 cM |
MW | 55.4 kDa |
Gene Summary | Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218752 | Rnf14 (untagged) - Mouse ring finger protein 14 (Rnf14), transcript variant 1, (10ug) |
USD 503.00 |
|
MG218588 | Rnf14 (tGFP-tagged) - Mouse ring finger protein 14 (Rnf14) transcript variant 1, (10ug) |
USD 703.00 |
|
MR218588L3 | Lenti ORF clone of Rnf14 (Myc-DDK-tagged) - Mouse ring finger protein 14 (Rnf14), transcript variant 1 |
USD 803.00 |
|
MR218588L4 | Lenti ORF clone of Rnf14 (mGFP-tagged) - Mouse ring finger protein 14 (Rnf14), transcript variant 1 |
USD 803.00 |
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