Tcn2 (NM_001130459) Mouse Tagged ORF Clone
CAT#: MR218492
- TrueORF®
Tcn2 (Myc-DDK-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001130459" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tcn2 |
Synonyms | AW208754; Tcn-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218492 representing NM_001130459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCTGAAGGCGCTGCTGCTCCTTTCTGGAGTCTTTGGGGCTCTTGCTGAATTCTGTGTAATAC CACGGATAGACAGCCAGCTGGTAGAGAAGTTGGGTCAACGTCTTTTACCTTGGATGGATCGGCTTTCCTC AGAGCAGTTGAACCCCAGCGTCTTCGTTGGCCTGCGACTTTCCAGCATGCAGGCTGGGACCAAAGAGGAC CTCTACCTTCACAGCCTCAAGATCCATTACCAGCAGTGCCTCCTGCGGTCCACCTCCAGCGATGATAACA GTAGTTGCCAGCCCAAACTTTCCGGGGGCAGCCTGGCGCTCTACCTGCTTGCTCTCAGGGCCAACTGCGA ATTCTTCGGGAGCCGAAAGGGTGACAGGTTGATCTCACAGCTCAAGTGGTTCTTGGAGGATGAGAAGAAG GCCATTGGGCACAATCATGAAGGCCACCCCAACACCAACTATTACCAGTATGGCCTCAGCATCCTGGCAC TGTGTGTCCACCAGAAGCGGCTCCATGACAGCGTGGTGGGCAAGCTCCTGTATGCTGTTGAACATGACTA CTTTACCTACCAGGGCCACGTCTCTGTGGACACAGAAGCCATGGCCGGCTTGGCCCTCACCTGTTTGGAG CGATTCAATTTCAACTCTGATCTGAGACCACGAATCACCATGGCTATCGAGACAGTGCGAGAGAAAATCC TGAAGTCCCAGGCCCCTGAGGGCTACTTTGGGAATATCTATAGCACCCCACTGGCACTACAGATGCTCAT GACATCTCCTGCATCCGGGGTAGGGCTGGGCACAGCGTGCATCAAGGCAGGGACATCCCTGCTGCTCAGC CTACAGGATGGGGCCTTCCAAAATCCTCTGATGATTTCCCAGCTGCTACCCATCCTGAACCACAAGACCT ACTTGGATCTTATCTTCCCAGACTGCCAGGCATCCAGGGTCATGTTGGTGCCAGCTGTTGAGGACCCTGT CCACATCTCAGAAGTCATCAGCGTCACGCTGAAGGTCGCCAGTGCCCTGTCACCATACGAACAGACATTT TTTGTCTTTGCTGGATCTTCCTTGGAAGATGTCCTGAAGTTGGCACAAGATGGCGGAGGATTCACGTATG GGACCCAGGCCTCCTTGTCAGGCCCCTACCTGACATCTGTACTAGGGAAAGATGCTGGGGACCGAGAATA TTGGCAGCTCCTCCGAGCCCCTGACACACCCCTGCTGCAAGGCATTGCTGACTACAAACCCCAGGACGGG GAAACCATTGAGCTGCGGCTAGTTAGATGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218492 representing NM_001130459
Red=Cloning site Green=Tags(s) MELLKALLLLSGVFGALAEFCVIPRIDSQLVEKLGQRLLPWMDRLSSEQLNPSVFVGLRLSSMQAGTKED LYLHSLKIHYQQCLLRSTSSDDNSSCQPKLSGGSLALYLLALRANCEFFGSRKGDRLISQLKWFLEDEKK AIGHNHEGHPNTNYYQYGLSILALCVHQKRLHDSVVGKLLYAVEHDYFTYQGHVSVDTEAMAGLALTCLE RFNFNSDLRPRITMAIETVREKILKSQAPEGYFGNIYSTPLALQMLMTSPASGVGLGTACIKAGTSLLLS LQDGAFQNPLMISQLLPILNHKTYLDLIFPDCQASRVMLVPAVEDPVHISEVISVTLKVASALSPYEQTF FVFAGSSLEDVLKLAQDGGGFTYGTQASLSGPYLTSVLGKDAGDREYWQLLRAPDTPLLQGIADYKPQDG ETIELRLVRW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130459 |
ORF Size | 1290 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130459.1, NP_001123931.1 |
RefSeq Size | 2030 bp |
RefSeq ORF | 1293 bp |
Locus ID | 21452 |
UniProt ID | O88968 |
Cytogenetics | 11 2.76 cM |
MW | 47.6 kDa |
Gene Summary | Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209500 | Tcn2 (untagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 3, (10ug) |
USD 503.00 |
|
MG218492 | Tcn2 (tGFP-tagged) - Mouse transcobalamin 2 (Tcn2) transcript variant 3, (10ug) |
USD 657.00 |
|
MR218492L3 | Lenti ORF clone of Tcn2 (Myc-DDK-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 3 |
USD 757.00 |
|
MR218492L4 | Lenti ORF clone of Tcn2 (mGFP-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 3 |
USD 757.00 |
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