Pfkfb1 (NM_008824) Mouse Tagged ORF Clone
CAT#: MR218288
- TrueORF®
Pfkfb1 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008824" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pfkfb1 |
Synonyms | PFK/FBPase 1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218288 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAAAAGCCTCTAAGAGAGCAGCCTCCATACCACAGTTCACTAATTCCCCCACAATGGTGATCA TGGTGGGTTTACCAGCTCGAGGCAAGACCTACATCTCTACGAAGCTCACACGCTATCTCAACTGGATAGG AACACCGACTAAAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGCTACAGGAACTATGAATTC TTTCGCCCAGACAACATGGAGGCCCAACTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAGGATGTCC ATAAGTATCTCAGCCGTGAGGAAGGTCACGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACG ATCATTGATTCTGCAGTTTGCTAAAGAACATGGTTACAAGGTCTTCTTTATTGAGTCTATTTGTAATGAC CCAGACATCATTGCAGAAAACATCAAGCAAGTGAAACTCGGCAGTCCTGATTACATAGACTGTGACCAAG AAAAGGTTTTAGAAGACTTTCTAAAGAGAATTGAGTGCTATGAGATCAACTACCAGCCTTTGGATGAGGA ATTGGACAGCCACCTGTCCTACATCAAGATCTTCGATGTGGGCACACGCTACATGGTAAATCGAGTGCAA GACCACGTTCAAAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATCTACCTAT GCCGACATGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAGCTCGGGG CAAGCAGTATGCCTATGCCCTGGCCAACTTCATTCGGTCTCAAAGCATCAGCTCCCTGAAGGTGTGGACT AGCCACATGAAGAGGACCATTCAGACAGCTGAAGCCCTAGGCGTCCCCTATGAACAGTGGAAGGCCCTGA ATGAGATTGATGCGGGTGTCTGTGAAGAGATGACTTATGAAGAAATTCAGGAACACTACCCTGAAGAATT TGCACTACGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAG CGTCTTGAGCCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAGGCTGTCA TGCGGTGCCTTCTAGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTCCTCTGCA TACAGTGCTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGTGGAGGCT GTGAACACACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAACCTGAGGAAGCCCTGGACACTG TCCCTGCCCATTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218288 protein sequence
Red=Cloning site Green=Tags(s) MEEKASKRAASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEF FRPDNMEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICND PDIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQ DHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQSISSLKVWT SHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQ RLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEA VNTHRDKPENVDITREPEEALDTVPAHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008824 |
ORF Size | 1347 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008824.1, NM_008824.2, NM_008824.3, NP_032850.1 |
RefSeq Size | 1753 bp |
RefSeq ORF | 1347 bp |
Locus ID | 18639 |
UniProt ID | P70266 |
Cytogenetics | X F3 |
MW | 52.1 kDa |
Gene Summary | Synthesis and degradation of fructose 2,6-bisphosphate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215959 | Pfkfb1 (untagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), (10ug) |
USD 503.00 |
|
MG218288 | Pfkfb1 (tGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-26-biphosphatase 1 (Pfkfb1), (10ug) |
USD 657.00 |
|
MR218288L3 | Lenti ORF clone of Pfkfb1 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1) |
USD 757.00 |
|
MR218288L4 | Lenti ORF clone of Pfkfb1 (mGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1) |
USD 757.00 |
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