Pfkfb1 (NM_008824) Mouse Tagged ORF Clone

CAT#: MR218288

Pfkfb1 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1)

Product Images

Circular map for MR218288

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb1
• Synonyms: PFK/FBPase 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218288 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAAAAGCCTCTAAGAGAGCAGCCTCCATACCACAGTTCACTAATTCCCCCACAATGGTGATCA
  TGGTGGGTTTACCAGCTCGAGGCAAGACCTACATCTCTACGAAGCTCACACGCTATCTCAACTGGATAGG
  AACACCGACTAAAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGCTACAGGAACTATGAATTC
  TTTCGCCCAGACAACATGGAGGCCCAACTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAGGATGTCC
  ATAAGTATCTCAGCCGTGAGGAAGGTCACGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACG
  ATCATTGATTCTGCAGTTTGCTAAAGAACATGGTTACAAGGTCTTCTTTATTGAGTCTATTTGTAATGAC
  CCAGACATCATTGCAGAAAACATCAAGCAAGTGAAACTCGGCAGTCCTGATTACATAGACTGTGACCAAG
  AAAAGGTTTTAGAAGACTTTCTAAAGAGAATTGAGTGCTATGAGATCAACTACCAGCCTTTGGATGAGGA
  ATTGGACAGCCACCTGTCCTACATCAAGATCTTCGATGTGGGCACACGCTACATGGTAAATCGAGTGCAA
  GACCACGTTCAAAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATCTACCTAT
  GCCGACATGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAGCTCGGGG
  CAAGCAGTATGCCTATGCCCTGGCCAACTTCATTCGGTCTCAAAGCATCAGCTCCCTGAAGGTGTGGACT
  AGCCACATGAAGAGGACCATTCAGACAGCTGAAGCCCTAGGCGTCCCCTATGAACAGTGGAAGGCCCTGA
  ATGAGATTGATGCGGGTGTCTGTGAAGAGATGACTTATGAAGAAATTCAGGAACACTACCCTGAAGAATT
  TGCACTACGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAG
  CGTCTTGAGCCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAGGCTGTCA
  TGCGGTGCCTTCTAGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTCCTCTGCA
  TACAGTGCTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGTGGAGGCT
  GTGAACACACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAACCTGAGGAAGCCCTGGACACTG
  TCCCTGCCCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218288 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEKASKRAASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEF
  FRPDNMEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICND
  PDIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQ
  DHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQSISSLKVWT
  SHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQ
  RLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEA
  VNTHRDKPENVDITREPEEALDTVPAHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008824
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008824.1, NM_008824.2, NM_008824.3, NP_032850.1
• RefSeq Size: 1753 bp
• RefSeq ORF: 1347 bp
• Locus ID: 18639
• UniProt ID: P70266
• Cytogenetics: X F3
• MW: 52.1 kDa
• Gene Summary: Synthesis and degradation of fructose 2,6-bisphosphate.[UniProtKB/Swiss-Prot Function]