Def6 (NM_027185) Mouse Tagged ORF Clone
CAT#: MR216879
- TrueORF®
Def6 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 6 (Def6)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_027185" in other vectors (6)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Def6 |
Synonyms | 2410003F05Rik; 6430538D02Rik; AV094905; Ibp; Slat; Slat2; Slat6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216879 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGCGCAAGGAGCTGCTCAAGTCTATCTGGTACGCCTTCACTGCACTGGACGTGGAGAAGAGCG GCAAGGTCTCCAAGTCCCAACTCAAGGTGCTGTCCCACAACCTGTACACTGTCCTGAACATCCCCCATGA CCCCGTGGCCCTGGAGGAGCACTTCCGGGATGACGATGATGGCCCGGTGTCCAGTCAAGGTTACATGCCC TACCTCAACAAGTACATCCTAGACAAGGTGGAGGAGGGAGCTTTCGTTAAGGAGCACTTCGATGAGTTGT GCTGGACCCTGACTGCCAAGAAGAACTACAGGGCAGATGGCATCGGGAGCAGCCCACTCTCTAATCAGGA TGCTTTCCGTCTATGGTGCCTCTTCAACTTCCTGTCTGAGGACAAGTACCCGCTGATCATGGTTCCCGAT GAGGTAGAGTATCTGCTGAAGAAGCTCCTTGGCAGCCTGAGTTTGGAGATGGGCCTGGGCAAGCTGGAGG AGCTGCTGGCCCAGGACGCCCAGTCAGCCCAGACCGCCGTGGGGCTCAGCGTCTGGCAGTTTCTGGAACT CTTCAACTCAGGCCGCTGCCTTCGGGGTGTGGGGCGCGACTCCCTCAGCATGGCCATCCAAGAAGTCTAC CAGGAGCTCATCCAAGACGTCCTGAAGCAGGGCTATCTGTGGAAGCGAGGGCACCTGCGGAGGAACTGGG CCGAGCGCTGGTTCCAGCTGCAACCCAGCAGCCTCTGCTACTTTGGGAGTGAGGAATGCAAGGAGAAACG AGGCACCATTCCCCTGGATGCTCACTGCTGTGTGGAGGTGCTTCCGGACCGCGAAGGAAAGCGCTGCATG TTTTGTGTGAAGACTGCCAGCCGCACCTATGAGATGAGCGCCTCAGACACCCGCCAGCGCCAGGAGTGGA CGGCCGCCATCCAGACTGCGATCCGGCTGCAGGCGGAGGGGAAGACGTCGCTGCACAAGGACCTGAAACA GAAGCGGCGGGAGCAGCGGGAACAGCGCGAGCGACGCCGGGCAGCCAAGGAGGAGGAGCTGCTGCGACTG CAGCAGCTGCAGGAGGAGAAGGAGAGGAAGCTGCAAGAACTGGAGCTGCTGCAGGAGGCTCAGCGGCAGG CCGAGCGGCTGCTGCAGGAAGAGGAGGAGCGCCGCCGTAGCCAGCACAAGGAGCTGCAGCAGGCTCTGGA GGGCCAGCTGCGGGAGGCGGAGCAGGCCCGGGCCTCTATGCAGGCTGAGATGGAGCTGAAGAAGGAAGAG GCGGCCCGGCAACGGCAGCGCATCGCTGAACTGGAGGAGATGCAGGAGCGGCTGCAGGAAGCTCTGCAAC TAGAGGTGAAAGCTAGGCGGGATGAGGAGGCCGTGCGCCTCGCCCAGACCAGGCTGCTGGAGGAGGAGGA GGAGAAGCTGAAGCAGCTGATGCATCTGAAGGAGGAGCAAGAGCGCTACATCGAGCGAGCGCAGCAGGAG AAGCAGGAGCTCCAGCAGGAGATGGCGCTGCAGAGCCGTTCCCTGCAGCACGCCCAGCAGCAGCTGGAGG AAGTGCGGCAGAACCGGCAGAGGGCAGACGAGGACGTGGAGGCTGCCCAGAGGAAGTTGCGCCAGGCCAG CACCAACGTGAAACACTGGAATGTCCAGATGAACAGGCTCATGCATCCGATCGAGCCAGGAGATAAGCGA CCCACCACCAGCAGCTCCTTCACGGGCTTCCAGCCCCCTCCGCTTGCCCGCCGGGACTCCTCTCTAAAGC GCCTGACCCGCTGGGGTTCCCAAGGTAACAGAACCCTCTCAGTCAATAGCAGCGAACAGAAGTCCCTCAA TGGTGGAGATGAGACTCCCATCCTAGCTTTGGCCTCTCAGGAAGAAAAACTGGATCCAGCACCAGGGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216879 protein sequence
Red=Cloning site Green=Tags(s) MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLYTVLNIPHDPVALEEHFRDDDDGPVSSQGYMP YLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRADGIGSSPLSNQDAFRLWCLFNFLSEDKYPLIMVPD EVEYLLKKLLGSLSLEMGLGKLEELLAQDAQSAQTAVGLSVWQFLELFNSGRCLRGVGRDSLSMAIQEVY QELIQDVLKQGYLWKRGHLRRNWAERWFQLQPSSLCYFGSEECKEKRGTIPLDAHCCVEVLPDREGKRCM FCVKTASRTYEMSASDTRQRQEWTAAIQTAIRLQAEGKTSLHKDLKQKRREQREQRERRRAAKEEELLRL QQLQEEKERKLQELELLQEAQRQAERLLQEEEERRRSQHKELQQALEGQLREAEQARASMQAEMELKKEE AARQRQRIAELEEMQERLQEALQLEVKARRDEEAVRLAQTRLLEEEEEKLKQLMHLKEEQERYIERAQQE KQELQQEMALQSRSLQHAQQQLEEVRQNRQRADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIEPGDKR PTTSSSFTGFQPPPLARRDSSLKRLTRWGSQGNRTLSVNSSEQKSLNGGDETPILALASQEEKLDPAPGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027185 |
ORF Size | 1893 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027185.3, NP_081461.2 |
RefSeq Size | 2294 bp |
RefSeq ORF | 1893 bp |
Locus ID | 23853 |
UniProt ID | Q8C2K1 |
Cytogenetics | 17 A3.3 |
MW | 73.5 kDa |
Gene Summary | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling. Required for optimal T-cell effector function, lymphocyte homeostasis and the prevention of systemic autoimmunity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219909 | Def6 (untagged) - Mouse differentially expressed in FDCP 6 (Def6), (10ug) |
USD 939.00 |
|
MG216879 | Def6 (tGFP-tagged) - Mouse differentially expressed in FDCP 6 (Def6), (10ug) |
USD 1,081.00 |
|
MR216879L1 | Lenti ORF clone of Def6 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 6 (Def6) |
USD 1,181.00 |
|
MR216879L2 | Lenti ORF clone of Def6 (mGFP-tagged) - Mouse differentially expressed in FDCP 6 (Def6) |
USD 1,181.00 |
|
MR216879L3 | Lenti ORF clone of Def6 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 6 (Def6) |
USD 1,181.00 |
|
MR216879L4 | Lenti ORF clone of Def6 (mGFP-tagged) - Mouse differentially expressed in FDCP 6 (Def6) |
USD 1,181.00 |
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