Llgl1 (NM_008502) Mouse Tagged ORF Clone
CAT#: MR216634
- TrueORF®
Llgl1 (Myc-DDK-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008502" in other vectors (5)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Llgl1 |
Synonyms | AI325176; Lgl1; Llglh; Mgl1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216634 representing NM_008502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTTAAGCAGGAGCTCTTCG CCTTCCACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCTTGGCCTTCGATCCTGAGCTCCG CATCATGGCCATCGGCACCAGATCTGGGGCCGTCAAGATCTATGGTGCACCCGGAGTGGAATTTACAGGC CTACATCGGGACGCAGCCACCGTCACCCAGATGCATTTCCTCCCTGGTCAGGGCCGCCTCCTGACCCTGC TAGATGACAGCAGCTTGCATCTCTGGGAGATCATCCATCATAATGGCTGCGCCCACCTGGAGGAAGGCCT CAGCTTCCACCCACCCAGCAGACCCAGTTTTGACAATGCCAGTTTCCCTGCCAGTCTAACACGTGTCACT GTGGTCCTGCTCGTAGCTGGCAATACAGCAGCCCTGGGAACTGAGAGTGGTAGCATATTCTTCCTGGATG TAGCCACCCTGGCACTGCTGGAGGGGCAGACTCTCAGCCCAGATGTGGTTCTGCGCAGTGTGCCAGATGA TTACCGGTGTGGAAAGGCCTTGGGCCCTGTGGAGTCACTCCAGGGACATCTGCAAGACCCCAGCAAGATC CTCATAGGCTACAGTCGGGGTTTACTGGTCATCTGGAGCCAGGCCACACAGTCTGTGGACAACGTTTTCC TAGGTAACCAGCAGCTGGAGAGCCTGTGTTGGGGCCGTGATGGCAGCAGCATTATCAGCTCACACAGTGA TGGCAGCTATGCCATCTGGTCCACAGACACTGGCAGCCCCCCAACGCTGCAGCCCACTGTAGTGACCACA CCCTACGGCCCCTTCCCCTGCAAGGCCATCAACAAGATTCTGTGGCGGAGCTGTGAGTCAGGAGACCACT TTATCATCTTCAGTGGTGGCATGCCTCGAGCCAGCTATGGTGACCGCCACTGTGTGAGTGTACTGCGAGC AGAGACACTGGTGACCCTGGACTTCACCTCTCGTGTCATTGACTTCTTCACGGTGCACAGCACACAGCCA GAGGATGAGTGTGACAACCCCCAGGCCTTAGCCGTGCTTCTGGAGGAGGAGCTGGTGGTGCTGGACCTGC AGACACCAGGCTGGCCAGCTGTGCCCGCCCCTTACCTGGCCCCACTGCATTCGTCAGCTATCACCTGCTC TGCCCATGTTGCCAATGTCCCCAGCAAGCTGTGGGCCCGCATTGTAAGTGCTGGTGAGCAGCAGAGCCCA CAGCCTGCCTCCAGTGCCTTGAGTTGGCCCATTACCGGGGGCCGGAACTTGGCCCAGGAACCCTCGCAGC GTGGGCTGCTGCTCACTGGCCATGAGGATGGCACTGTGCGGTTCTGGGACGCCTCTGGTGTGGCGCTAAG GCCACTCTACAAACTGAGCACAGCTGGCCTCTTCCAGACGGACTGTGAACATGCTGACAGCCTGGCCCAG GCTGTGGAGGATGACTGGCCGCCCTTCCGCAAGGTGGGCTGCTTTGATCCCTACAGTGATGACCCCCGGC TAGGAATCCAGAAGGTTGCGCTTTGCAAGTACACAGCCCAGATGGTGGTAGCTGGCACTGCAGGCCAGGT GCTGGTGCTGGAGCTCAGTGAGGTCCCAGCAGAGCATGCCGTCAGTGTGGCCAACGTGGATCTTCTTCAG GATCGCGAGGGCTTCACGTGGAAGGGTCACGAGCGGCTGAACCCACACACGGGGCTGCTGCCGTGGCCTG CCGGATTCCAGCCCCGCATGCTGATACAGTGCCTCCCACCCGCCGCTGTCACTGCTGTCACACTCCATGC TGAGTGGAGCCTCGTGGCCTTTGGTACCAGTCATGGCTTTGGCCTTTTTGACTACCAGCGCAAGAGCCCT GTGCTGGCTAGGTGCACCCTTCACCCCAATGATTCTTTGGCCATGGAGGGGCCACTGTCACGGGTGAAGT CCCTCAAGAAGTCACTGAGACAGTCATTCCGGCGAATCCGCAAGAGCCGTGTCTCAGGCAAAAAACGGAC TCCTGCTGCCAGTAGCAAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGACCTGCCCACACGACCTG GAGATGACACCCGTGCAGCGCCGCATTGAGCCTCGGTCTGCTGACGACTCGCTCTCCGGTGTTGTACGCT GCCTCTACTTTGCTGACACGTTCCTTCGAGATGCGACCCACCACGGGCCCACCATGTGGGCGGGCACCAA CTCGGGCTCTGTGTTCGCCTATGCGCTGGAGGTTCCAGCAGCCACAGCAGGCGGAGAGAAGCGGCCTGAG CAGGCAGTGGAGGCGGTGCTGGGCAAGGAGGTGCAGCTAATGCACCGAGCACCTGTGGTGGCCATTGCTG TGCTGGATGGTCGTGGCCGGCCACTGCCTGAGCCCTATGAGGCTTCCCGGGACCTGGCCCAGGCGCCAGA CATGCAAGGCGGCCATGCTGTGCTCATTGCATCTGAGGAACAATTCAAGGTGTTCACACTACCCAAGGTG AGTGCTAAGACTAAATTCAAGCTTACAGCCCATGAAGGCTGTCGTGTGCGGAAGGTAGCCCTGGCTACAT TTGCCAGCGTGATGTCTGAGGACTATGCCGAGACCTGCCTTGCCTGCCTCACCAACCTGGGTGATGTCCA CGTCTTCTCGGTGCCTGGCCTGAGGCCTCAGGTGCACTACTCCTGTATCCGGAAGGAGGACATCAGTGGC ATCGCTTCCTGTGTCTTCACACGTCACGGCCAGGGCTTTTACTTGATTTCTCCATCGGAATTTGAGCGCT TCTCACTGAGTGCTCGCAACATCACAGAACCACTATGTTCTCTGGATATAAGCTGGCCCCAAAATGCCAC CCAGCCCAGGCTTCAAGAGTCACCCAAGCTGAGCCAGGCTAATGGGACCAGAGACATCATTCTGGCCCCA GAGAGCTGCGAAGGAAGCCCTAGCTCTGCCCACAGCAAGCGAGCTGATACCATGGAACCACCCGAGGCCG CTCTCTCGCCTGTGTCCATTGATTCAGCTGCTAGTGGGGATACCATGCTGGACACAACAGGGGATGTCAC CGTGGAATATGTGAAGGATTTTCTGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216634 representing NM_008502
Red=Cloning site Green=Tags(s) MMKFRFRRQGADPQREKLKQELFAFHKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTG LHRDAATVTQMHFLPGQGRLLTLLDDSSLHLWEIIHHNGCAHLEEGLSFHPPSRPSFDNASFPASLTRVT VVLLVAGNTAALGTESGSIFFLDVATLALLEGQTLSPDVVLRSVPDDYRCGKALGPVESLQGHLQDPSKI LIGYSRGLLVIWSQATQSVDNVFLGNQQLESLCWGRDGSSIISSHSDGSYAIWSTDTGSPPTLQPTVVTT PYGPFPCKAINKILWRSCESGDHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRVIDFFTVHSTQP EDECDNPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVANVPSKLWARIVSAGEQQSP QPASSALSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLAQ AVEDDWPPFRKVGCFDPYSDDPRLGIQKVALCKYTAQMVVAGTAGQVLVLELSEVPAEHAVSVANVDLLQ DREGFTWKGHERLNPHTGLLPWPAGFQPRMLIQCLPPAAVTAVTLHAEWSLVAFGTSHGFGLFDYQRKSP VLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRTPAASSKLQEANAQLAEQTCPHDL EMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDATHHGPTMWAGTNSGSVFAYALEVPAATAGGEKRPE QAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPKV SAKTKFKLTAHEGCRVRKVALATFASVMSEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDISG IASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDISWPQNATQPRLQESPKLSQANGTRDIILAP ESCEGSPSSAHSKRADTMEPPEAALSPVSIDSAASGDTMLDTTGDVTVEYVKDFLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008502 |
ORF Size | 3102 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008502.1, NM_008502.2, NP_032528.1 |
RefSeq Size | 4419 bp |
RefSeq ORF | 3105 bp |
Locus ID | 16897 |
UniProt ID | Q80Y17 |
Cytogenetics | 11 37.81 cM |
MW | 112.8 kDa |
Gene Summary | Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223272 | Llgl1 (untagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1), transcript variant 2, (10ug) |
USD 991.00 |
|
MG211492 | Llgl1 (tGFP-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1) |
USD 1,103.00 |
|
MG216634 | Llgl1 (tGFP-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1) transcript variant 2, (10ug) |
USD 1,190.00 |
|
MR216634L3 | Lenti ORF clone of Llgl1 (Myc-DDK-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1), transcript variant 2 |
USD 1,290.00 |
|
MR216634L4 | Lenti ORF clone of Llgl1 (mGFP-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1), transcript variant 2 |
USD 1,290.00 |
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