St6galnac6 (NM_001025311) Mouse Tagged ORF Clone

CAT#: MR216393

St6galnac6 (Myc-DDK-tagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 3

Product Images

Circular map for MR216393

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St6galnac6
• Synonyms: Siat7f; ST6GalNAcVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216393 representing NM_001025311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAACTCAGGGGCCCCCTCCTTGGACACTGTGGGTCCGGTACCTGCTGGAAGGAGCAGCGGTCAG
  CAGTGTTTGTGATCCTCTTTGCCCTCATCACCATCCTCATCCTCTACAGCTCCAACAGTGCCAACGAGGT
  CTTCCACTACGGCTCCCTGCGGGGCCGCACGCGTCGGCCAGTCAACCTCAAGAAGTGGAGTTTCTCCAGC
  GCCTACTTCCCTATCCTCGGCAACAAGACGCTGCCGTCCAGGTGCAACCAGTGTGTGATCATCACCAGCT
  CCAGCCACCTGCTGGGCACCAAACTGGGCCCTGAGATTGAGCGGGCTGAGTGCACCATCCGCATGAACGA
  TGCTCCCACCTCTGGCTACTCGGCTGACGTCGGAAACAAAACCACCTTCCGCGTAGTGGCCCATTCCAGT
  GTATTCCGTGTGCTGCGGAAGCCCCAGGAATTTGTCAACCGGACCCCTGAGACGGTGTTCATCTTCTGGG
  GACCCCCAAACAAGATGCAGAAGCCCCAGGGCAGCCTCCTGCGCGTCATCCAGCGGGCGGGCCTCATGTT
  CCCGAACATGGAGGCCTATGCCGTCTCTCCCGCCCGCATGCAGCAGTTTGACGACCTCTTCCGGGGTGAG
  ACGGGCAAGGACAGGGAAAAGTCCCATTCCTGGTTGAGCACGGGCTGGTTTACCATGGTGATTGCGGTGG
  AATTGTGTGACCATGTGCACGTGTATGGCATGGTCCCTCCTGACTACTGCAGCCAGCGGCCCCGCCTGCA
  GCGCATGCCATACCACTACTATGAACCCAAGGGGCCGGACGAGTGTGTCACCTACATCCAGAACGAGCAC
  AGCCGTAAGGGCAATCACCACCGCTTCATCACCGAGAAGAGGGTCTTCTCGTCCTGGGCCCAACTCTACG
  GTATCACCTTCTCCCACCCCTCCTGGACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216393 representing NM_001025311
  Red=Cloning site Green=Tags(s)
  
  MMKLRGPLLGHCGSGTCWKEQRSAVFVILFALITILILYSSNSANEVFHYGSLRGRTRRPVNLKKWSFSS
  AYFPILGNKTLPSRCNQCVIITSSSHLLGTKLGPEIERAECTIRMNDAPTSGYSADVGNKTTFRVVAHSS
  VFRVLRKPQEFVNRTPETVFIFWGPPNKMQKPQGSLLRVIQRAGLMFPNMEAYAVSPARMQQFDDLFRGE
  TGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPDYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEH
  SRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025311
• ORF Size: 939 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025311.2, NP_001020482.1
• RefSeq Size: 2288 bp
• RefSeq ORF: 942 bp
• Locus ID: 50935
• UniProt ID: Q9JM95
• Cytogenetics: 2 B
• MW: 36.3 kDa
• Gene Summary: Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no activity toward glycoproteins. Responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in kidney.[UniProtKB/Swiss-Prot Function]