Nek9 (NM_145138) Mouse Tagged ORF Clone
CAT#: MR216306
- TrueORF®
Nek9 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_145138" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Nek9 |
Synonyms | C130021H08Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216306 representing NM_145138
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTCGGAAGCGAGTCCGGGG GTGGCGGGGACTCGGGCCCGGGCCCCAGCGCTGTCCCAGGGCCGCGAGCCGGCGGCGGGGCGGCGGAGCA GGAGGAGCTGCACTACATCCCCATCCGCGTTCTGGGCCGCGGTGCCTTTGGGGAGGCCACGCTGTACCGC CGCACCGAGGACGACTCACTGGTGGTGTGGAAGGAAGTTGACTTGACCCGGCTATCTGAGAAGGAACGTC GAGATGCCCTGAATGAGATTGTCATCCTGGCACTGCTACAGCACGACAACATCATTGCCTACTACAACCA CTTCATGGACAACACCACCCTGCTGATTGAGCTGGAGTACTGTAACGGAGGGAACCTCTATGACAAAATC CTTCGTCAGAAGGACAAGTTGTTCGAAGAAGAGATGGTGGTGTGGTACCTTTTTCAGATTGTTTCAGCAG TGAGCTGTATTCATAAAGCTGGAATCCTCCATAGAGATATAAAGACATTAAATATTTTTCTGACCAAGGC AAATCTGATAAAGCTTGGAGACTATGGCCTAGCAAAGAAACTTAATTCTGAGTACTCTATGGCCGAGACA CTTGTGGGCACTCCATATTACATGTCTCCAGAGCTCTGCCAAGGAGTAAAATACAATTTCAAGTCTGATA TATGGGCAGTTGGCTGTGTCATTTTTGAACTGCTTACTCTGAAAAGAACATTTGATGCTACAAACCCACT CAACCTATGTGTGAAGATCGTGCAGGGAATCCGGGCCATGGAAGTTGACTCGAGTCAGTATTCTTTGGAA CTGATCCAGTTGGTACACGCATGCCTCGACCAGGATCCTGAGCAGAGGCCCGCTGCAGATGCACTCCTGG ATCTCCCCCTTCTCAGGACCCGCAGGAGAGAGATGGAGGAAAAAGTCACTCTGCTCAATGCACCTACAAA GAGACCAAGGTCAAGCACTGTGACGGAAGCACCCATTGCTGTGGTAACATCACGAACCAGTGAAGTCTAT GTTTGGGGTGGTGGAAAATCCACACCTCAGAAATTGGACGTCATCAAGAGTGGCTGTAGTGCTCGACAGG TGTGTGCGGGGAACACCCACTTTGCTGTGGTCACCGTGGAGAAGGAACTATATACCTGGGTGAACATGCA AGGGGGCACTAAACTTCACGGTCAGCTAGGACATGGAGACAAAGCTTCCTACCGACAGCCAAAGCATGTA GAAAAGTTACAAGGGAAGGCTATCCACCAGGTGTCGTGTGGGGATGATTTCACTGTCTGTGTCACAGATG AGGGTCAGCTCTACGCCTTTGGATCAGATTATTATGGCTGCATGGGAGTGGACAAGGTTTCTGGCCCTGA AGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAATCCTGTGGAGCAGGTCTCCTGTGGGGATAAT CATGTGGTAGTTCTGACACGAAACAAAGAAGTCTACTCTTGGGGCTGTGGTGAATATGGACGGCTGGGTT TGGATTCAGAAGAGGATTACTATACACCACAGAGGGTGGATGTTCCAAAGGCCTTAATCATTGTTGCAGT TCAATGTGGCTGTGATGGGACCTTTCTGCTCACTCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAG TTCAACAAGCTGGGGCTGAATCAGTGCATGTCTGGAATCATCAACCATGAAGCATACCATGAAGTTCCCT ACACAACCTCCTTTACCTTGGCCAAACAGTTGTCCTTTTACAAAATCCGTACCATTGCCCCAGGCAAGAC TCACACAGCTGCTATTGATGAACGAGGCCGGTTGCTGACTTTTGGCTGTAATAAGTGTGGGCAGCTGGGT GTTGGGAATTATAAGAAACGCCTGGGAATCAACCTGTTGGGGGGACCCCTTGGCGGGAAGCAAGTGATTA GGGTCTCGTGTGGCGATGAGTTCACCATTGCTGCCACCGACGATAATCACATCTTTGCCTGGGGTAATGG TGGTAATGGCCGCCTGGCAATGACTCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT CGGCCTATTTTTGGATCCCTGCATCATGTTCCAGACCTTTCTTGCCGTGGCTGGCACACCATTCTTATTG TTGAGAAAGTGTTGAATTCCAAGACCATCCGTTCCAATAGCAGTGGCCTGTCCATCGGAACTGTGGTTCA GAGCTCCAGCCCAGGAGGTGGCATTGGTGGTGGTGGTGGTGGTGGCGGCGGCGGCGGGGGTGAAGAGGAA GACAGTCAACAGGAATCTGAAACTCCAGATCCAAGCGGAGGCTTCCGAGGAACAATGGAAGCAGACCGTG GGATGGAAGGCTTAATCAGTCCCACGGAGGCCGTGGGAAACAGCTGTGGGGCTAGCAGCTCCTGTCCTGG CTGGCTTCGGAAGGAGCTGGAAAACGCAGAGTTCATTCCCATGCCTGACAGCCCCGCTCCCTTAAGTGCG GCGTTTTCACAGTCTGAGAAGGACACGCTGCCCTATGAAGAGCTGCAGGGACTCAAGGTCGCATCTGAAG TTCCTCCGGAGCCCCAGCGTGCAGCAGGAGCCTGGCCCCCTCGGCTGGATCCTGCAGTGCCGTGTGTTGG GAAAGCCCTGACCTCTGCTGCTTGTGCGTGCAGTGCTCTGCAGGTGGAGGTTGACAGGTTGCAGGCTCTG GTGTTAAAGTGTCTGGAAGAACAGCAGAAGCTCCAGCAAGAAAACCTCCAGATGTTTACCCAACTACAAA AGCTCAACAAGAAATTGGAAGGAGGCCAGCAGGTGGGGATGCACTCCAGAGGAACCCAGACAGCAAAGGA AGAAATGGAAATGGATCCAAAGCCTGACTTAGACTCGGAGTCCTGGTGCCTCCTCGGAACAGACTCTTGT CGACCCAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216306 representing NM_145138
Red=Cloning site Green=Tags(s) MSVLGEYERHCDSINSDFGSESGGGGDSGPGPSAVPGPRAGGGAAEQEELHYIPIRVLGRGAFGEATLYR RTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKI LRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAET LVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLE LIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVY VWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHV EKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDN HVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNE FNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLG VGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWP RPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEE DSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSA AFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQAL VLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSC RPSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145138 |
ORF Size | 2952 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145138.2, NP_660120.2 |
RefSeq Size | 5403 bp |
RefSeq ORF | 2955 bp |
Locus ID | 217718 |
UniProt ID | Q8K1R7 |
Cytogenetics | 12 D1 |
MW | 107.6 kDa |
Gene Summary | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222965 | Nek9 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug) |
USD 902.00 |
|
MG216306 | Nek9 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug) |
USD 1,551.00 |
|
MR216306L3 | Lenti ORF clone of Nek9 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9) |
USD 1,651.00 |
|
MR216306L4 | Lenti ORF clone of Nek9 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9) |
USD 1,651.00 |
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