Arhgef6 (NM_152801) Mouse Tagged ORF Clone

CAT#: MR216238

Arhgef6 (Myc-DDK-tagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6)

Product Images

Circular map for MR216238

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef6
• Synonyms: 1600028C08Rik; 1700038J06Rik; 4930592P22Rik; alpha-PIX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216238 representing NM_152801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATCACGGATGTGGCTTAGTCTTCACAGCTGCCCCGAGAGCTGTTCAGAGCGTCCGAGAGCCTCAG
  TCATGAATCCAGAAGAACGCCTTGTGACATGGCTTATATCTTTGGGAGTTCTAGAGTCTCCCAAAAAGAC
  TGTCTGTGATCCAGAGGAGTTCTTGAAGTCCTCGCTGAAAAATGGGGTAGTGTTGTGCAAACTCATAAAC
  AGACTTCTGCCTGGCTCGGTGGAGAAGTATTGTCTGGAACCCCAAACTGAGGCCGACTGCATCGACAACA
  TCAATGACTTCCTGAAAGGATGTGCAACTCTTCAAGTGGAAGTGTTTGAGCCTGATGACCTCTATTCGGG
  GGCCAATTTCTCCAAGGTTCTGAATACTCTTTTAGCTGTCAACAAAGCAACTGAAGATCAGCTCTCAGAA
  AGACCATGTGGAAGGTCCTCTTCTCTTAGTGCTGCGACCAGCTCTCAGACAAACCCGCAGGTGGCCGTTC
  CCAGCACAGCTCCAGAGCAGCACAGCGAGGAAAAGGCAGAGATGACGGAAAATGGAAGTCACCAGTTGAT
  AGTAAAGGCAAGATTCAACTTCAAGCAGACGAATGAAGATGAATTGTCAGTGTGTAAAGGGGACATCATT
  TATGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACTGGATGGTTCCCTA
  GTAATTATGTCCGAGAAATTAAACCCAGTGAGAGACCTCTGTCCCCTAAGGCTATCAAAGGATTTGACAC
  TGCTCCACTTACCAAGAATTACTACACTGTGGTGTTACAGAACATCTTGGACACTGAAAAAGAATATGCC
  AAAGAACTTCAGTCTCTTCTTGTTACTTACCTAAGACCCTTGCAGTCTAATAACAACCTGAGTACTGTGG
  AGTTTACATGTTTATTGGGAAACTTTGAAGAAGTATGCACATTTCAACAGACACTCTGCCAAGCCTTGGA
  AGAATGTTCAAAGTTTCCAGAAAACCAACACAAAGTAGGAGGCTGCCTACTGAACCTCATGCCTCATTTC
  AAATCGATGTACCTGGCTTACTGTGCAAATCATCCATCAGCTGTCAACGTGCTCACCCAGCACAGTGATG
  ATCTAGAACGATTCATGGAAAATCAAGGTGCATCCAGCCCGGGTATCCTCATTCTAACAACAAGTCTCAG
  CAAACCATTCATGCGACTGGAAAAGTATGTTACTCTCTTGCAAGAACTGGAACGACATATGGAGGACACT
  CATCCAGACCATCAGGATATTCTGAAAGCAATCATAGCATTCAAAACACTCATGGGGCAGTGTCAAGATC
  TGAGAAAGAGAAAGCAGCTGGAATTGCAGATCCTTTCAGAACCTATTCAGGCATGGGAAGGAGATGATAT
  TAAGACTTTGGGAAATGTGATCTTTATGTCTCAAGTAGTGATGCAGCATGGGGCCTGTGAGGAGAAAGAG
  GAACGATATTTTCTGCTGTTTTCAAGTGTCTTGATAATGTTGTCTGCAAGTCCTCGGATGAGTGGCTTTA
  TGTATCAGGGAAAAATACCAATAGCAGGGATGGTAGTGAACAGATTGGATGAAATTGAAGGCAGTGACTG
  TATGTTCGAAATCACAGGTAGCACGGTTGAGAGAATTGTGGTCCATTGCAACAATAACCAGGACTTCCAG
  GAGTGGATGGAGCAGCTGAACAGACTGACCAAAGGACCAACCTCTTGCGGTTCATTGTCTAAGACCTCCT
  CCTCATCATGTAGTACTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAGCCTCCTCA
  AATTATAAAACCGTGGAGTTTAAGTTGCCTACGACCTGCACCTCCACTTAGACCATCAGCAGCACTAGGT
  TATAAAGAGAGGATGTCTTATATCTTAAAGGAATCTAGTAAGAGCCCCAAAACAATGAAGAAGTTTCTTC
  ACAAAAGAAAGACGGAGAGGAAAGCATCCGAGGAAGAATACGTGATTAGGAAAAGTACTGCTGCCCTGGA
  AGAGGATGCTCAGATTCTTAAGGTGATCGAAGCTTATTGCACCAGCGCCAGTTTTCAACAAGGTACTCGT
  AAAGATTCTGTTCCACAAGTTCTACTCCCCGAGGAAGAGAAACTCATTATTGAAGAAACCAGAAGTAATG
  GGCAGACCATCATTGAAGAGAAGAGCCTTGTTGATACTGTCTATGCCTTGAAGGATGAGGTCAAGGAACT
  GAAGCAGGAGAATAAAAAAATGAAGCAATGTCTGGAAGAAGAATTGAAGTCGAGGAAGGACCTAGAGAAG
  CTGGTCCGGAAGCTGTTGAAACAAACAGATGAATGCATTCGATCTGAATCCAGCAGCAAGACCTCGATTC
  TCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216238 representing NM_152801
  Red=Cloning site Green=Tags(s)
  
  MQSRMWLSLHSCPESCSERPRASVMNPEERLVTWLISLGVLESPKKTVCDPEEFLKSSLKNGVVLCKLIN
  RLLPGSVEKYCLEPQTEADCIDNINDFLKGCATLQVEVFEPDDLYSGANFSKVLNTLLAVNKATEDQLSE
  RPCGRSSSLSAATSSQTNPQVAVPSTAPEQHSEEKAEMTENGSHQLIVKARFNFKQTNEDELSVCKGDII
  YVTRVEEGGWWEGTLNGRTGWFPSNYVREIKPSERPLSPKAIKGFDTAPLTKNYYTVVLQNILDTEKEYA
  KELQSLLVTYLRPLQSNNNLSTVEFTCLLGNFEEVCTFQQTLCQALEECSKFPENQHKVGGCLLNLMPHF
  KSMYLAYCANHPSAVNVLTQHSDDLERFMENQGASSPGILILTTSLSKPFMRLEKYVTLLQELERHMEDT
  HPDHQDILKAIIAFKTLMGQCQDLRKRKQLELQILSEPIQAWEGDDIKTLGNVIFMSQVVMQHGACEEKE
  ERYFLLFSSVLIMLSASPRMSGFMYQGKIPIAGMVVNRLDEIEGSDCMFEITGSTVERIVVHCNNNQDFQ
  EWMEQLNRLTKGPTSCGSLSKTSSSSCSTHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLRPSAALG
  YKERMSYILKESSKSPKTMKKFLHKRKTERKASEEEYVIRKSTAALEEDAQILKVIEAYCTSASFQQGTR
  KDSVPQVLLPEEEKLIIEETRSNGQTIIEEKSLVDTVYALKDEVKELKQENKKMKQCLEEELKSRKDLEK
  LVRKLLKQTDECIRSESSSKTSILQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152801
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152801.2, NP_690014.2
• RefSeq Size: 4580 bp
• RefSeq ORF: 2388 bp
• Locus ID: 73341
• UniProt ID: Q8K4I3
• Cytogenetics: X A6
• MW: 89.8 kDa
• Gene Summary: Acts as a RAC1 guanine nucleotide exchange factor (GEF).[UniProtKB/Swiss-Prot Function]