Tex2 (NM_198292) Mouse Tagged ORF Clone

CAT#: MR215727

Tex2 (Myc-DDK-tagged) - Mouse testis expressed gene 2 (Tex2)

Product Images

Circular map for MR215727

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tex2
• Synonyms: 4930568E07Rik; AI553404; Def-5; Taz4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215727 representing NM_198292
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGTCTGAATGGTCGCCATGCTGAGAAAACCATTGACATGCCAAAACCATCAGCCCCCAAAGTGC
  ACGTGCAAAGGTCTGTGTCCCGAGACACCATCGCCATTCATTTCTCGGCTTCCGGGGAGGAGGAGGAGGA
  AGAGGAGGAGGAGTTCAGGGGCTACCTGGAGGAGGGGCTAGATGACCAAAGCATTGTAACGGGGCTTGAA
  GCCAAGGAGGACCTGTATCTTGAATCCCAGGGTGGCCATGACCCTGCAGGCCCCGTCTCGACGGCCCCTG
  CAGATGGGCTGTCTGTGTCTGAGTCCCCTGCCATTTTGCCTGTCTCTGAGAACACTGTAAAGCTGCTGGA
  GTCCCCTGCTCCAGCACTGCAGGTATTAAGTCCAGTGCCATTGGCTCTGTCTCCAGGATCATCTTCATCT
  GGACCCTTAGCTAGTTCTCCCAGTGTGTCGTCCCTTTCTGAGCAGAAAACCAGTTCTTCGTCTCCACTGT
  CTTCTCCTTCCAAGTCCCCTGTCCTGTCCTCCAGTGCCTCGTCCTCGGCCCTCTCAAGCGCAAAGCCCTT
  CATGAGCCTGGTGAAGTCCCTGTCAACTGAGGTGGAGCCAAAAGAGTCCCCCCACCCCCCGAGGCACAGA
  CACTTGATGAAGACTCTGGTCAAGTCTCTGTCCACAGACACTTCCCGGCAGGAGTCGGACACCGTGTCCT
  ACAAGCCCCCCGACTCCAAACTCAACCTGCACCTCTTCAAACAGTTCACACAGCCACGGAACACGGGTGG
  AGACTCCAAGACTGCGCCTTCTTCCCCCCTGACTTCGCCCTCTGACACTCGTTCCTTTTTTAAAGTGCCC
  GAAATGGAGGCAAAAATCGAAGACACTAAGCGACGCCTTTCTGAAGTTATCTATGAGCCCTTCCAGCTCC
  TCAGCAAGATCATCGGGGAGGAGAGCGGCAGCCATAGGCCCAAAGCCTTATCTGCAAGTGCTTCAGAGCT
  CTCCAGCCTGTCCGGCTTGAACGGGCACTTGGAAAGCAATAACTACAGCATCAAGGAGGAGGAGGGGGAC
  TCGGAGGGGGAAGGCTACGGCAGCGACTCCAACACCTCCAGGAGCGACCACCTGAAGCCCACTGAAGACG
  CCTCGAAGGAAGTGGAGCCCAAGGGTTCCCAGGCGAGTAGTCTGAAGGACTTAGGTCTGAAGACAAGTTC
  TCTGGTTCTTGAGAAGTGTTCTCTGTCTGCTTTAGTGAGCAAGGAGGATGAAGAGTTCTGTGAACTGTAC
  ACGGAGGACTTTGAGCTGGAGACTGAGGGCGAGGGGAGACTTGATAAGACCCTCGATCTCCCCCTCAAGC
  CAGAGGTGCTTGCCAGCGATGGCGTGGCTCTGGAGAGCGAGGATGAAGAGGACTCGGCCACCGAGCACCA
  GGAGTTACCTGTAAAGACGCTCGGCTTCTTTATAATGTGTGTCTACGCGTACCTCATCCTCCCACTCCCC
  TACTACATGAGTGGGCTCTTCCTGGGCGTCGGCCTCGGCTTCATGACTGCAGTCTGCATGATTTGGTTCT
  TTACACCACCAAGTGCTCACAAACACCACAAATCACTCAAAGCTCTGCGGCACCAGAGCACGAGATCCCT
  GGACATCAAGGAGCCCGAAATACTAAAGGGATGGATGAATGAGATTTACAACTATGATCCAGAAACTTAC
  CACGCGACTTTGACGCACTCAGTCTTTGTCCGGCTCGAGGGTGGAACCTTAAGACTTTCAAAGCCCAACA
  AAAACATATCCAGGAGGGCAAGCTACAATGAGACAAAGCCAGAGGTCACCTACATCAGCCAGAAAATCTA
  TGACCTCTCAGACAGCAAGATTTATCTTGTACCTAAAAGCTTGGCTCGAAAGCGAATCTGGAATAAGAAG
  TACCCCATTTGCATTGAACTTGGTCGGCAAGATGACTTTATGTCTAAGGCGCAGTCTGATAAGGAAGCTA
  CTGAGGAGAAGCCGCCTCCTGAGAAAGAGCTGCCGAGCGAAGACCTCAAGAAGCCACCCCAGCCTCAAGA
  GGGGACGAAGTCTAGCCAGCGAGACCCAATACTGTATCTGTTTGGGAGAACGGGCCGAGAGAAAGAGGAG
  TGGTTCAGGCGGTTCATCCTGGCCTCGAGGCTGAAGTCAGAGCTCAGGAAGCCGGCGGGTGTTTCTGGAA
  GTAAATCAGGGCTTCTGCCCGCACACAGCAGGCACAGCAGTCCCTCGGGGCACCTGAGCCACAGCCGCAG
  CAGCAGCAAAGGCAGCGTGGAGGAAATGATGTCCCAGCCAAAGCAGAAGGAACTGGTGGGCAGCGTGCGA
  CAGAAGATGCTCCTAGACTACAGTGTGTACATGGGCAGGTGTGTGCCCCAGGACAACCGAAGCCCCCACA
  GGAGCCCCGTGCAGAGTGCAGAGAGCAGCCCCACAGCTTCGAAGAAGTTGCCAGAGGCCCCACCCTCTGA
  GGAGGAAGAACAGGAAGCCTGGGTGAATGCTTTGCTTGGAAGAATATTTTGGGACTTCTTGGGCGAGAAA
  TACTGGTCTGATGTGGTGTCGAAGAAAATCCAGATGAAACTCAGCAAAATTAAGCTCCCCTACTTTATGA
  ATGAACTAACACTGACAGAGCTTGACATGGGTGTAGCTGTGCCAAAAATCCTTCAGGCCTTCAAGCCTTA
  TGTTGATCACCAAGGACTCTGGATCGATTTGGAAATGTCTTACAATGGGTCCTTTCTGATGACTCTGGAA
  ACCAAAATGAACTTGACCAAACTAGGTAAAGAGCCTCTTGTTGAAGCCCTGAAGGTTGGAGAAATTGGCA
  AAGAAGGTTGCAGGCCCAGGGCATACTGTCTGGCTGACAGTGATGAAGAATCCTCCAGTGCTGGCTCCTC
  TGAGGAAGATGACCCACCAGAACCCACTGCTGGAGACAAACAGCCGCTCCCCGGGGCTGAAGGGTACGTT
  GGAGGTCATCGAACAAGCAAGATTATGAGGTTTGTTGATAAGATCACCAAATCAAAATATTTCCAAAAAG
  CAACAGAGACAGAATTCATTAAAAAGAAGATCGAAGAAGTCTCTAATACACCACTGCTGCTAACTGTGGA
  AGTGCAAGAGTGTCGAGGGACCTTGGCGGTCAACATCCCCCCACCACCGACTGACCGGATATGGTATGGT
  TTCCGGAAGCCACCGTATGTGGAGCTGAAAGCTCGGCCAAAACTTGGAGAGAGAGAAGTGACTTTAGTTC
  ACGTGACAGAGTGGATTGAGAAGAAACTGGAGCAGGAGCTTCAGAAAGTGTTTGTCATGCCAAACATGGA
  TGACGTTTATATTCCTATAATGCACTCGGCCATGGACCCTCGCTCCACTTCCTGCCTCTTGAAAGAGCCA
  CCTGTGGAGACCTCTGATCAGCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215727 representing NM_198292
  Red=Cloning site Green=Tags(s)
  
  MTSLNGRHAEKTIDMPKPSAPKVHVQRSVSRDTIAIHFSASGEEEEEEEEEFRGYLEEGLDDQSIVTGLE
  AKEDLYLESQGGHDPAGPVSTAPADGLSVSESPAILPVSENTVKLLESPAPALQVLSPVPLALSPGSSSS
  GPLASSPSVSSLSEQKTSSSSPLSSPSKSPVLSSSASSSALSSAKPFMSLVKSLSTEVEPKESPHPPRHR
  HLMKTLVKSLSTDTSRQESDTVSYKPPDSKLNLHLFKQFTQPRNTGGDSKTAPSSPLTSPSDTRSFFKVP
  EMEAKIEDTKRRLSEVIYEPFQLLSKIIGEESGSHRPKALSASASELSSLSGLNGHLESNNYSIKEEEGD
  SEGEGYGSDSNTSRSDHLKPTEDASKEVEPKGSQASSLKDLGLKTSSLVLEKCSLSALVSKEDEEFCELY
  TEDFELETEGEGRLDKTLDLPLKPEVLASDGVALESEDEEDSATEHQELPVKTLGFFIMCVYAYLILPLP
  YYMSGLFLGVGLGFMTAVCMIWFFTPPSAHKHHKSLKALRHQSTRSLDIKEPEILKGWMNEIYNYDPETY
  HATLTHSVFVRLEGGTLRLSKPNKNISRRASYNETKPEVTYISQKIYDLSDSKIYLVPKSLARKRIWNKK
  YPICIELGRQDDFMSKAQSDKEATEEKPPPEKELPSEDLKKPPQPQEGTKSSQRDPILYLFGRTGREKEE
  WFRRFILASRLKSELRKPAGVSGSKSGLLPAHSRHSSPSGHLSHSRSSSKGSVEEMMSQPKQKELVGSVR
  QKMLLDYSVYMGRCVPQDNRSPHRSPVQSAESSPTASKKLPEAPPSEEEEQEAWVNALLGRIFWDFLGEK
  YWSDVVSKKIQMKLSKIKLPYFMNELTLTELDMGVAVPKILQAFKPYVDHQGLWIDLEMSYNGSFLMTLE
  TKMNLTKLGKEPLVEALKVGEIGKEGCRPRAYCLADSDEESSSAGSSEEDDPPEPTAGDKQPLPGAEGYV
  GGHRTSKIMRFVDKITKSKYFQKATETEFIKKKIEEVSNTPLLLTVEVQECRGTLAVNIPPPPTDRIWYG
  FRKPPYVELKARPKLGEREVTLVHVTEWIEKKLEQELQKVFVMPNMDDVYIPIMHSAMDPRSTSCLLKEP
  PVETSDQL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198292
• ORF Size: 3384 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198292.3, NP_938034.2
• RefSeq Size: 4947 bp
• RefSeq ORF: 3387 bp
• Locus ID: 21763
• UniProt ID: Q6ZPJ0
• Cytogenetics: 11 69.46 cM
• MW: 125.7 kDa
• Gene Summary: During endoplasmic reticulum (ER) stress or when cellular ceramide levels increase, may induce contacts between the ER and medial-Golgi complex to facilitate non-vesicular transport of ceramides from the ER to the Golgi complex where they are converted to complex sphingolipids, preventing toxic ceramide accumulation.[UniProtKB/Swiss-Prot Function]