Npas3 (NM_013780) Mouse Tagged ORF Clone

CAT#: MR215623

Npas3 (Myc-DDK-tagged) - Mouse neuronal PAS domain protein 3 (Npas3)

Product Images

Circular map for MR215623

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Npas3
• Synonyms: 4930423H22Rik; bHLHe12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215623 representing NM_013780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGGCCGGCGCCGCGGCCAACGGCACCCCGCAGAACGTCCAGGGCATCACCTCCTACCAGCAGC
  GAATAACTGCCCAGCATCCTCTGCCCAACCAATCAGAATGTAGGAAAATCTACAGATATGACGGAATCTA
  CTGTGAATCTACCTACCAGAATTTACAAGCACTGAGAAAGGAGAAATCCAGAGATGCTGCTCGATCCCGC
  CGGGGAAAAGAAAATTTTGAGTTCTATGAATTGGCCAAGCTGTTGCCTCTTCCTGCCGCCATCACCAGCC
  AGCTCGACAAGGCATCCATCATCCGACTTACAATTAGTTATCTCAAGATGAGAGACTTTGCCAACCAAGG
  GGATCCTCCATGGAACCTGAGGATGGAAGGCCCTCCACCAAACACCTCAGTAAAAGGTGCACAACGAAGG
  CGGAGCCCCAGCGCACTGGCCATTGAAGTATTTGAAGCACATTTGGGAAGCCACATTTTGCAGTCCCTGG
  ATGGCTTCGTATTTGCACTAAATCAAGAAGGAAAATTTTTGTACATTTCTGAAACCGTCTCTATCTACCT
  AGGCCTCTCACAAGTGGAGCTGACAGGTAGCAGCGTCTTTGACTATGTCCACCCTGGGGACCACGTGGAG
  ATGGCAGAGCAGCTGGGCATGAAGCTCCCGCCTGGGCGGGGTCTCCTCTCGCAGGGCACCACAGAGGACG
  CGGCCAGTTCAGCATCTTCCTCTTCTCAGTCAGAGACCCCTGAGCCAGTGGAGACGACCAGCCCTAGTCT
  GCTAACCACGGACAACACCCTTGAACGGTCCTTTTTCATCCGAATGAAGTCTACTCTGACCAAACGTGGA
  GTCCACATCAAGTCATCTGGGTATAAGGTGATTCACATAACAGGCCGGCTTCGCCTCAGAGTGTCGCTGT
  CCCACGGGAGGACCGTCCCCAGCCAAATCATGGGTCTGGTGGTTGTGGCTCACGCCTTGCCTCCCCCTAC
  GATCAATGAAGTCAGAATTGACTGCCATATGTTCGTCACTCGAGTAAACATGGATCTCAATATCATTTAC
  TGTGAAAATAGGATTAGTGATTACATGGATCTGACCCCTGTAGACATCGTAGGGAAAAGATGCTACCACT
  TCATCCATGCCGAAGATGTAGAAGGCATCAGGCACAGTCACCTGGACTTGCTGAATAAGGGTCAGTGTGT
  GACCAAGTACTACCGCTGGATGCAGAAGAATGGAGGCTACATCTGGATACAGTCTAGTGCCACCATAGCT
  ATTAATGCGAAGAATGCAAATGAGAAGAACATCATCTGGGTGAATTATCTTCTTAGTAATCCGGAGTACA
  AGGACACACCCATGGACATTGCGCAACTCCCGCATTTGCCAGAGAAAGCGTCAGAATCCTCAGAGACATC
  CGACTCTGAGTCAGACTCTAAAGACACCTCAGGTATTACAGAGGACAATGAGAACTCCAAGTCCGACGAG
  AAGGGCAATCAGTCCGAGAATAGCGAAGATCCGGAGCCTGACCGCAAGAAGTCAGGCAGCGCATGCGACA
  ACGACATGAACTGCAACGACGATGGCCACAGCTCCAGCAACCCGGACAGCCGTGACAGCGACGACAGCTT
  CGAGCACTCGGACTTTGAACACCCCAAGGCGGCCGAGGATGGCTTCGGCGCGCTGGGCCCTATGCAGATC
  AAAGTGGAGCGCTACGTGGAGAGCGAGGCGGACCTGCGACTGCAGCCCTGCGAGTCGCTCACGTCGGACA
  GCGCCAAGGACTCAGACAGCGCGAACGAGGCGGGCGCTCAGGCATCCAGCAAGCACCAGAAGCGCAAGCG
  GCGGCGGAAACGGCAGAAGGGCGGCAGCGCCAGTCGCCGACGCCTGTCCAGCGCGTCCAGCCCAGGCCTG
  GACGCCGGTCTGGTGGAGCCTCCGCGGCTGCTGTCATCGCCGCACAGCGCCTCTGTGCTCAAGATCAAGA
  CAGAGATCGCCGAGCCCATCAACTTCGACAACGACAGCAGCATCTGGAACTACCCGCCCAACCGGGAGAT
  CTCCAGGAACGAATCCCCGTACAGCATGACCAAGCCCCCCACCTCCGAGCACTTCCCGTCCCCGCAGGGC
  CAGGGAGGCAGCAGCGGTGGCGGGGGAGCGCTGCACGTGGCCATCCCCGACTCGGTCCTCACCCCACCGG
  GCGCCGATGGCACAGCTGGCCGCAAGACTCAGTTCAGCGGCACAGCCCCGGTGCCCTCTGATCCACTGTC
  GCCCCCGCTCTCTGCGTCCCCGCGGGACAAGCACCCCGGGGGCGGGGCGGGGAGTGGCGGGGGTGGCCCT
  GGTGCATCCAACTCCTTGCTGTACACTGGGGACCTGGAGGCCCTGCAGAGGTTGCAAGCGGGCAACGTCG
  TGCTTCCGCTGGTGCACCGGGTGACCGGGACCCTGGCAGCCACCAGCACAGCCGCACAGAGGGTCTACAC
  CACGGGCACTATCCGCTACGCACCCGCTGAGGTGACCCTGGCCATGCAGGGCAACCTGCTACCCAACGCG
  CATGCTGTTAACTTCGTGGACGTTAACAGCCCAGGCTTTGGCCTCGACCCCAAGACACCCATGGAGATGC
  TCTACCACCACGTGCACCGACTCAACATGTCAGGACCATTTGGCGGCGCCGTGAGCGCGGCCAGCCTCAC
  ACAGATGCCAGGTGGCAACGTGTTCACCACAGCCGAGGGACTCTTCTCCACGCTGCCCTTCCCAGTCTAC
  AGCAACGGTATCCACGCGGCACAGACTCTGGAGCGCAAGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215623 representing NM_013780
  Red=Cloning site Green=Tags(s)
  
  MGRAGAAANGTPQNVQGITSYQQRITAQHPLPNQSECRKIYRYDGIYCESTYQNLQALRKEKSRDAARSR
  RGKENFEFYELAKLLPLPAAITSQLDKASIIRLTISYLKMRDFANQGDPPWNLRMEGPPPNTSVKGAQRR
  RSPSALAIEVFEAHLGSHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVELTGSSVFDYVHPGDHVE
  MAEQLGMKLPPGRGLLSQGTTEDAASSASSSSQSETPEPVETTSPSLLTTDNTLERSFFIRMKSTLTKRG
  VHIKSSGYKVIHITGRLRLRVSLSHGRTVPSQIMGLVVVAHALPPPTINEVRIDCHMFVTRVNMDLNIIY
  CENRISDYMDLTPVDIVGKRCYHFIHAEDVEGIRHSHLDLLNKGQCVTKYYRWMQKNGGYIWIQSSATIA
  INAKNANEKNIIWVNYLLSNPEYKDTPMDIAQLPHLPEKASESSETSDSESDSKDTSGITEDNENSKSDE
  KGNQSENSEDPEPDRKKSGSACDNDMNCNDDGHSSSNPDSRDSDDSFEHSDFEHPKAAEDGFGALGPMQI
  KVERYVESEADLRLQPCESLTSDSAKDSDSANEAGAQASSKHQKRKRRRKRQKGGSASRRRLSSASSPGL
  DAGLVEPPRLLSSPHSASVLKIKTEIAEPINFDNDSSIWNYPPNREISRNESPYSMTKPPTSEHFPSPQG
  QGGSSGGGGALHVAIPDSVLTPPGADGTAGRKTQFSGTAPVPSDPLSPPLSASPRDKHPGGGAGSGGGGP
  GASNSLLYTGDLEALQRLQAGNVVLPLVHRVTGTLAATSTAAQRVYTTGTIRYAPAEVTLAMQGNLLPNA
  HAVNFVDVNSPGFGLDPKTPMEMLYHHVHRLNMSGPFGGAVSAASLTQMPGGNVFTTAEGLFSTLPFPVY
  SNGIHAAQTLERKED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013780
• ORF Size: 2775 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013780.2, NP_038808.2
• RefSeq Size: 5879 bp
• RefSeq ORF: 2778 bp
• Locus ID: 27386
• Cytogenetics: 12 C1
• MW: 100.9 kDa